Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats

Autores
Deyurka, Nicolás Andrés; Navigatore Fonzo, Lorena Silvina; Coria Lucero, Cinthia Daiana; Lacoste, Maria Gabriela; Anzulovich, A. C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
RORα is a transcription factor that binds RORE motifs in the promoter of clock Bmal1 and othertarget genes. Antioxidant enzymes contribute to the cellular redox state which is crucial for themolecular clock function. Previously, we showed antioxidant enzymes activity follow a dailyvariation in temporal cortex (TC), which was abolished in aged rats. Herein we aimed: 1) toinvestigate endogenous rhythms of Cat, Gpx and Nrf2 genes expression and RORα protein levels inrat TC, and 2) to evaluate whether aging could affect those temporal patterns. Three- and 22-monthold male Holtzman rats were maintained under constant darkness for 15 days before the experiment,in order to validate the endogenous nature of circadian rhythms. TC samples were isolated every 4h during a 24h period. RORα protein levels were assessed by immunoblotting. Cat, Gpx and Nrf2 mRNA levels were determined by RT-PCR. Specific softwares were used to circadian analysis. We observed circadian endogenous rhythms of RORα, Cat and Gpx expression in the TC of young freerunning rats (Chronos fit, p<0.05, p< 0.001 and p<0.05, respectively). We found Ebox and RORE sites in the Cat and Gpx genes regulatory regions. RORα rhythm?s acrophase occurs at the beginning of the subjective day, preceding Cat and Gpx mRNA peaks. Consistent with previous results, aging abolishes RORα, Cat, and Gpx circadian rhythms in TC. Interestingly, Nrf2 gene expression becomes rhythmic in the TC of aged rats. Our observations would contribute to the knowledge of circadian alterations in TC of the aged brain.
Fil: Deyurka, Nicolás Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich, A. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo
Villa de Merlo
Argentina
Sociedad de Biología de Cuyo
Materia
AGING
CIRCADIAN RHYTHM
RORa
CAT
GPX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199923
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running ratsDeyurka, Nicolás AndrésNavigatore Fonzo, Lorena SilvinaCoria Lucero, Cinthia DaianaLacoste, Maria GabrielaAnzulovich, A. C.AGINGCIRCADIAN RHYTHMRORaCATGPXhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1RORα is a transcription factor that binds RORE motifs in the promoter of clock Bmal1 and othertarget genes. Antioxidant enzymes contribute to the cellular redox state which is crucial for themolecular clock function. Previously, we showed antioxidant enzymes activity follow a dailyvariation in temporal cortex (TC), which was abolished in aged rats. Herein we aimed: 1) toinvestigate endogenous rhythms of Cat, Gpx and Nrf2 genes expression and RORα protein levels inrat TC, and 2) to evaluate whether aging could affect those temporal patterns. Three- and 22-monthold male Holtzman rats were maintained under constant darkness for 15 days before the experiment,in order to validate the endogenous nature of circadian rhythms. TC samples were isolated every 4h during a 24h period. RORα protein levels were assessed by immunoblotting. Cat, Gpx and Nrf2 mRNA levels were determined by RT-PCR. Specific softwares were used to circadian analysis. We observed circadian endogenous rhythms of RORα, Cat and Gpx expression in the TC of young freerunning rats (Chronos fit, p<0.05, p< 0.001 and p<0.05, respectively). We found Ebox and RORE sites in the Cat and Gpx genes regulatory regions. RORα rhythm?s acrophase occurs at the beginning of the subjective day, preceding Cat and Gpx mRNA peaks. Consistent with previous results, aging abolishes RORα, Cat, and Gpx circadian rhythms in TC. Interestingly, Nrf2 gene expression becomes rhythmic in the TC of aged rats. Our observations would contribute to the knowledge of circadian alterations in TC of the aged brain.Fil: Deyurka, Nicolás Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Anzulovich, A. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaXXXV Reunión Científica Anual de la Sociedad de Biología de CuyoVilla de MerloArgentinaSociedad de Biología de CuyoInstituto de Histología y Embriología2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199923Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 82-821667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2018/05/Biocell_.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:37Zoai:ri.conicet.gov.ar:11336/199923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:37.602CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
title Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
spellingShingle Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
Deyurka, Nicolás Andrés
AGING
CIRCADIAN RHYTHM
RORa
CAT
GPX
title_short Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
title_full Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
title_fullStr Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
title_full_unstemmed Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
title_sort Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats
dc.creator.none.fl_str_mv Deyurka, Nicolás Andrés
Navigatore Fonzo, Lorena Silvina
Coria Lucero, Cinthia Daiana
Lacoste, Maria Gabriela
Anzulovich, A. C.
author Deyurka, Nicolás Andrés
author_facet Deyurka, Nicolás Andrés
Navigatore Fonzo, Lorena Silvina
Coria Lucero, Cinthia Daiana
Lacoste, Maria Gabriela
Anzulovich, A. C.
author_role author
author2 Navigatore Fonzo, Lorena Silvina
Coria Lucero, Cinthia Daiana
Lacoste, Maria Gabriela
Anzulovich, A. C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGING
CIRCADIAN RHYTHM
RORa
CAT
GPX
topic AGING
CIRCADIAN RHYTHM
RORa
CAT
GPX
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv RORα is a transcription factor that binds RORE motifs in the promoter of clock Bmal1 and othertarget genes. Antioxidant enzymes contribute to the cellular redox state which is crucial for themolecular clock function. Previously, we showed antioxidant enzymes activity follow a dailyvariation in temporal cortex (TC), which was abolished in aged rats. Herein we aimed: 1) toinvestigate endogenous rhythms of Cat, Gpx and Nrf2 genes expression and RORα protein levels inrat TC, and 2) to evaluate whether aging could affect those temporal patterns. Three- and 22-monthold male Holtzman rats were maintained under constant darkness for 15 days before the experiment,in order to validate the endogenous nature of circadian rhythms. TC samples were isolated every 4h during a 24h period. RORα protein levels were assessed by immunoblotting. Cat, Gpx and Nrf2 mRNA levels were determined by RT-PCR. Specific softwares were used to circadian analysis. We observed circadian endogenous rhythms of RORα, Cat and Gpx expression in the TC of young freerunning rats (Chronos fit, p<0.05, p< 0.001 and p<0.05, respectively). We found Ebox and RORE sites in the Cat and Gpx genes regulatory regions. RORα rhythm?s acrophase occurs at the beginning of the subjective day, preceding Cat and Gpx mRNA peaks. Consistent with previous results, aging abolishes RORα, Cat, and Gpx circadian rhythms in TC. Interestingly, Nrf2 gene expression becomes rhythmic in the TC of aged rats. Our observations would contribute to the knowledge of circadian alterations in TC of the aged brain.
Fil: Deyurka, Nicolás Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Coria Lucero, Cinthia Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich, A. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo
Villa de Merlo
Argentina
Sociedad de Biología de Cuyo
description RORα is a transcription factor that binds RORE motifs in the promoter of clock Bmal1 and othertarget genes. Antioxidant enzymes contribute to the cellular redox state which is crucial for themolecular clock function. Previously, we showed antioxidant enzymes activity follow a dailyvariation in temporal cortex (TC), which was abolished in aged rats. Herein we aimed: 1) toinvestigate endogenous rhythms of Cat, Gpx and Nrf2 genes expression and RORα protein levels inrat TC, and 2) to evaluate whether aging could affect those temporal patterns. Three- and 22-monthold male Holtzman rats were maintained under constant darkness for 15 days before the experiment,in order to validate the endogenous nature of circadian rhythms. TC samples were isolated every 4h during a 24h period. RORα protein levels were assessed by immunoblotting. Cat, Gpx and Nrf2 mRNA levels were determined by RT-PCR. Specific softwares were used to circadian analysis. We observed circadian endogenous rhythms of RORα, Cat and Gpx expression in the TC of young freerunning rats (Chronos fit, p<0.05, p< 0.001 and p<0.05, respectively). We found Ebox and RORE sites in the Cat and Gpx genes regulatory regions. RORα rhythm?s acrophase occurs at the beginning of the subjective day, preceding Cat and Gpx mRNA peaks. Consistent with previous results, aging abolishes RORα, Cat, and Gpx circadian rhythms in TC. Interestingly, Nrf2 gene expression becomes rhythmic in the TC of aged rats. Our observations would contribute to the knowledge of circadian alterations in TC of the aged brain.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199923
Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 82-82
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199923
identifier_str_mv Aging abolishes circadian rhythms of RORα and antioxidant enzymes expression in temporal cortex of free running rats; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; Villa de Merlo; Argentina; 2017; 82-82
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2018/05/Biocell_.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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