Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395

Autores
Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Lorenzo Pisarello, Maria Jose; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant lectins, which are proteins/glycoproteins present in a wide range of vegetables, fruits, cereals and beans, are resistant to digestive enzymes and food cooking temperatures. They bind reversibly to specific glycosidic residues expressed on the membrane of intestinal epithelial cells (IEC) and cause anti-nutritional effects in humans and animals. Soybean lectin (SBA) has been detected in poultry diets, and its ability to bind to the intestinal epithelium has been reported. The development of new methods for removing SBA from feeds or to prevent interaction with the intestinal mucosa is of interest. In this study, the in vitro cytotoxicity of SBA on IEC of chicks was demonstrated for the first time. The LD50, assessed after 2 h exposure of IEC to SBA, was 6.13 μg mL-1. The ability of Bifidobacterium infantis CRL1395 to bind SBA on the bacterial envelope was confirmed, and prevention of IEC cytotoxicity by lectin removal was demonstrated. Safety of B. infantis CRL1395, resistance to gastrointestinal stress and adhesion were also determined. It was concluded that the early administration of B. infantis CRL1395 to chicks would effectively reduce the toxicity of SBA. Besides, it would favour the colonization of the gut with a beneficial microbiota.
Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Argañaraz Martínez, Fernando Eloy. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzo Pisarello, Maria Jose. Universidad Nacional de Tucumán; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
Materia
Bifidobacterium
Broiler Chicks
Cytotoxicity
Intestinal Epithelial Cells
Probiotics
Soybean Agglutinin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36753
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36753
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395Babot, Jaime DanielArgañaraz Martínez, Fernando EloyLorenzo Pisarello, Maria JoseApella, Maria CristinaPerez Chaia, Adriana BeatrizBifidobacteriumBroiler ChicksCytotoxicityIntestinal Epithelial CellsProbioticsSoybean Agglutininhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Plant lectins, which are proteins/glycoproteins present in a wide range of vegetables, fruits, cereals and beans, are resistant to digestive enzymes and food cooking temperatures. They bind reversibly to specific glycosidic residues expressed on the membrane of intestinal epithelial cells (IEC) and cause anti-nutritional effects in humans and animals. Soybean lectin (SBA) has been detected in poultry diets, and its ability to bind to the intestinal epithelium has been reported. The development of new methods for removing SBA from feeds or to prevent interaction with the intestinal mucosa is of interest. In this study, the in vitro cytotoxicity of SBA on IEC of chicks was demonstrated for the first time. The LD50, assessed after 2 h exposure of IEC to SBA, was 6.13 μg mL-1. The ability of Bifidobacterium infantis CRL1395 to bind SBA on the bacterial envelope was confirmed, and prevention of IEC cytotoxicity by lectin removal was demonstrated. Safety of B. infantis CRL1395, resistance to gastrointestinal stress and adhesion were also determined. It was concluded that the early administration of B. infantis CRL1395 to chicks would effectively reduce the toxicity of SBA. Besides, it would favour the colonization of the gut with a beneficial microbiota.Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Argañaraz Martínez, Fernando Eloy. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lorenzo Pisarello, Maria Jose. Universidad Nacional de Tucumán; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; ArgentinaWiley Blackwell Publishing, Inc2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36753Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Lorenzo Pisarello, Maria Jose; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 363; 12; 6-2016; 1-70378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/363/12/fnw114/2570349info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnw114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:39Zoai:ri.conicet.gov.ar:11336/36753instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:39.303CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
title Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
spellingShingle Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
Babot, Jaime Daniel
Bifidobacterium
Broiler Chicks
Cytotoxicity
Intestinal Epithelial Cells
Probiotics
Soybean Agglutinin
title_short Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
title_full Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
title_fullStr Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
title_full_unstemmed Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
title_sort Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395
dc.creator.none.fl_str_mv Babot, Jaime Daniel
Argañaraz Martínez, Fernando Eloy
Lorenzo Pisarello, Maria Jose
Apella, Maria Cristina
Perez Chaia, Adriana Beatriz
author Babot, Jaime Daniel
author_facet Babot, Jaime Daniel
Argañaraz Martínez, Fernando Eloy
Lorenzo Pisarello, Maria Jose
Apella, Maria Cristina
Perez Chaia, Adriana Beatriz
author_role author
author2 Argañaraz Martínez, Fernando Eloy
Lorenzo Pisarello, Maria Jose
Apella, Maria Cristina
Perez Chaia, Adriana Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bifidobacterium
Broiler Chicks
Cytotoxicity
Intestinal Epithelial Cells
Probiotics
Soybean Agglutinin
topic Bifidobacterium
Broiler Chicks
Cytotoxicity
Intestinal Epithelial Cells
Probiotics
Soybean Agglutinin
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Plant lectins, which are proteins/glycoproteins present in a wide range of vegetables, fruits, cereals and beans, are resistant to digestive enzymes and food cooking temperatures. They bind reversibly to specific glycosidic residues expressed on the membrane of intestinal epithelial cells (IEC) and cause anti-nutritional effects in humans and animals. Soybean lectin (SBA) has been detected in poultry diets, and its ability to bind to the intestinal epithelium has been reported. The development of new methods for removing SBA from feeds or to prevent interaction with the intestinal mucosa is of interest. In this study, the in vitro cytotoxicity of SBA on IEC of chicks was demonstrated for the first time. The LD50, assessed after 2 h exposure of IEC to SBA, was 6.13 μg mL-1. The ability of Bifidobacterium infantis CRL1395 to bind SBA on the bacterial envelope was confirmed, and prevention of IEC cytotoxicity by lectin removal was demonstrated. Safety of B. infantis CRL1395, resistance to gastrointestinal stress and adhesion were also determined. It was concluded that the early administration of B. infantis CRL1395 to chicks would effectively reduce the toxicity of SBA. Besides, it would favour the colonization of the gut with a beneficial microbiota.
Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Argañaraz Martínez, Fernando Eloy. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lorenzo Pisarello, Maria Jose. Universidad Nacional de Tucumán; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán; Argentina
description Plant lectins, which are proteins/glycoproteins present in a wide range of vegetables, fruits, cereals and beans, are resistant to digestive enzymes and food cooking temperatures. They bind reversibly to specific glycosidic residues expressed on the membrane of intestinal epithelial cells (IEC) and cause anti-nutritional effects in humans and animals. Soybean lectin (SBA) has been detected in poultry diets, and its ability to bind to the intestinal epithelium has been reported. The development of new methods for removing SBA from feeds or to prevent interaction with the intestinal mucosa is of interest. In this study, the in vitro cytotoxicity of SBA on IEC of chicks was demonstrated for the first time. The LD50, assessed after 2 h exposure of IEC to SBA, was 6.13 μg mL-1. The ability of Bifidobacterium infantis CRL1395 to bind SBA on the bacterial envelope was confirmed, and prevention of IEC cytotoxicity by lectin removal was demonstrated. Safety of B. infantis CRL1395, resistance to gastrointestinal stress and adhesion were also determined. It was concluded that the early administration of B. infantis CRL1395 to chicks would effectively reduce the toxicity of SBA. Besides, it would favour the colonization of the gut with a beneficial microbiota.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36753
Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Lorenzo Pisarello, Maria Jose; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 363; 12; 6-2016; 1-7
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36753
identifier_str_mv Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Lorenzo Pisarello, Maria Jose; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Cytotoxic damage of soybean agglutinin on intestinal epithelial cells of broiler chicks: In vitro protection by Bifidobacterium infantis CRL1395; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 363; 12; 6-2016; 1-7
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/363/12/fnw114/2570349
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnw114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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