Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide

Autores
Tortella, G. R.; Salgado, E.; Cuozzo, Sergio Antonio; Mella Herrera, R. A.; Parra, L.; Diez, M. C.; Rubilar, O.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Combined microbiological test (Biolog Ecoplate, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were developed to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of an pesticide biopurification system (PBS). Moreover, pesticide dissipation was also evaluated. After three successive exposition of DZN, dissipation efficiency was high; achieved 87%, 93% and 96% after each application, respectively showing a clear accelerated dissipation of this pesticide in the organic matrix. The results obtained with Biolog Ecoplate showed that community level physiological profiles were no affected by the addition of DZN. On the other hand, molecular assays (DGGE and QPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. Therefore, the results of the present study, clearly, demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.
Fil: Tortella, G. R.. Universidad de la Frontera. Nucleo Cientifico y Tecnologico En Recursos Naturales (bioren-ufro). Departamento de Ciencias Quimicas y Recursos Naturales; Chile
Fil: Salgado, E.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Mella Herrera, R. A.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Parra, L.. Universidad de la Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Diez, M. C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Rubilar, O.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Materia
BIOPURIFICATION SYSTEM
MICROBIAL COMMUNITIES
DIAZINON
DISSIPATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17176
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17176
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticideTortella, G. R.Salgado, E.Cuozzo, Sergio AntonioMella Herrera, R. A.Parra, L.Diez, M. C.Rubilar, O.BIOPURIFICATION SYSTEMMICROBIAL COMMUNITIESDIAZINONDISSIPATIONhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Combined microbiological test (Biolog Ecoplate, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were developed to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of an pesticide biopurification system (PBS). Moreover, pesticide dissipation was also evaluated. After three successive exposition of DZN, dissipation efficiency was high; achieved 87%, 93% and 96% after each application, respectively showing a clear accelerated dissipation of this pesticide in the organic matrix. The results obtained with Biolog Ecoplate showed that community level physiological profiles were no affected by the addition of DZN. On the other hand, molecular assays (DGGE and QPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. Therefore, the results of the present study, clearly, demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.Fil: Tortella, G. R.. Universidad de la Frontera. Nucleo Cientifico y Tecnologico En Recursos Naturales (bioren-ufro). Departamento de Ciencias Quimicas y Recursos Naturales; ChileFil: Salgado, E.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Mella Herrera, R. A.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Parra, L.. Universidad de la Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Diez, M. C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileFil: Rubilar, O.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; ChileUniversidad de La Frontera2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17176Tortella, G. R.; Salgado, E.; Cuozzo, Sergio Antonio; Mella Herrera, R. A.; Parra, L.; et al.; Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide; Universidad de La Frontera; Journal of soil science and plant nutrition; 14; 4; 12-2014; 869-8800718-9516enginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vccnmbinfo:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-95162014005000069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:46Zoai:ri.conicet.gov.ar:11336/17176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:46.321CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
title Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
spellingShingle Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
Tortella, G. R.
BIOPURIFICATION SYSTEM
MICROBIAL COMMUNITIES
DIAZINON
DISSIPATION
title_short Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
title_full Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
title_fullStr Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
title_full_unstemmed Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
title_sort Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide
dc.creator.none.fl_str_mv Tortella, G. R.
Salgado, E.
Cuozzo, Sergio Antonio
Mella Herrera, R. A.
Parra, L.
Diez, M. C.
Rubilar, O.
author Tortella, G. R.
author_facet Tortella, G. R.
Salgado, E.
Cuozzo, Sergio Antonio
Mella Herrera, R. A.
Parra, L.
Diez, M. C.
Rubilar, O.
author_role author
author2 Salgado, E.
Cuozzo, Sergio Antonio
Mella Herrera, R. A.
Parra, L.
Diez, M. C.
Rubilar, O.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOPURIFICATION SYSTEM
MICROBIAL COMMUNITIES
DIAZINON
DISSIPATION
topic BIOPURIFICATION SYSTEM
MICROBIAL COMMUNITIES
DIAZINON
DISSIPATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Combined microbiological test (Biolog Ecoplate, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were developed to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of an pesticide biopurification system (PBS). Moreover, pesticide dissipation was also evaluated. After three successive exposition of DZN, dissipation efficiency was high; achieved 87%, 93% and 96% after each application, respectively showing a clear accelerated dissipation of this pesticide in the organic matrix. The results obtained with Biolog Ecoplate showed that community level physiological profiles were no affected by the addition of DZN. On the other hand, molecular assays (DGGE and QPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. Therefore, the results of the present study, clearly, demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.
Fil: Tortella, G. R.. Universidad de la Frontera. Nucleo Cientifico y Tecnologico En Recursos Naturales (bioren-ufro). Departamento de Ciencias Quimicas y Recursos Naturales; Chile
Fil: Salgado, E.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Mella Herrera, R. A.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Parra, L.. Universidad de la Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Diez, M. C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
Fil: Rubilar, O.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico En Recursos Naturales; Chile
description Combined microbiological test (Biolog Ecoplate, denaturing gradient gel electrophoresis (DGGE) and Real Time PCR (qPCR)) were developed to evaluate the impact of repeated diazinon (DZN) applications at high concentration (40 mg kg-1) on microbial communities in a microcosm simulating the organic matrix (straw (50%): peat (25%): soil (25%) vv-1) of an pesticide biopurification system (PBS). Moreover, pesticide dissipation was also evaluated. After three successive exposition of DZN, dissipation efficiency was high; achieved 87%, 93% and 96% after each application, respectively showing a clear accelerated dissipation of this pesticide in the organic matrix. The results obtained with Biolog Ecoplate showed that community level physiological profiles were no affected by the addition of DZN. On the other hand, molecular assays (DGGE and QPCR) demonstrated that the microbial structure (bacteria and fungi) remained relatively stable over time with high DZN doses compared to control. Therefore, the results of the present study, clearly, demonstrate the high dissipation capacity of this biomixture and highlight the microbiological robustness of this biological system.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17176
Tortella, G. R.; Salgado, E.; Cuozzo, Sergio Antonio; Mella Herrera, R. A.; Parra, L.; et al.; Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide; Universidad de La Frontera; Journal of soil science and plant nutrition; 14; 4; 12-2014; 869-880
0718-9516
url http://hdl.handle.net/11336/17176
identifier_str_mv Tortella, G. R.; Salgado, E.; Cuozzo, Sergio Antonio; Mella Herrera, R. A.; Parra, L.; et al.; Combined microbiological test to assess changes in an organic matrix used to avoid agricultural soil contamination, exposed to an insecticide; Universidad de La Frontera; Journal of soil science and plant nutrition; 14; 4; 12-2014; 869-880
0718-9516
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vccnmb
info:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-95162014005000069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad de La Frontera
publisher.none.fl_str_mv Universidad de La Frontera
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082587858042880
score 13.22299

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