Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture

Autores
Briceño, G.; Schalchli, H.; Rubilar, O.; Tortella, G.R.; Mutis, A.; Benimeli, Claudia Susana; Palma, G.; Diez, M.C.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Actinobacteria identified as Streptomyces spp. were evaluated for their ability to remove diazinon as the only carbon source from a liquid medium. Single cultures of Streptomyces strains were exposed to diazinon at a concentration of 50 mg L-1. After 96 h incubation, six of the eight cultures grew and five strains showed an increase in their total protein concentrations and changes in their protein profile. Up to 32% of the diazinon was removed by the single Streptomyces cultures. A compatibility assay showed that the different Streptomyces species were not antagonistic. Twenty-six mixed cultures were then prepared. Diazinon removal was increased when mixed cultures were used, and maximum diazinon removal of 62% was observed when the Streptomyces spp. strains AC5, AC9, GA11 and ISP13 were mixed; this was defined as the selected mixed culture (SMC). Diazinon removal was positively influenced by the addition of glucose into the liquid medium. Our study showed a diazinon degradation rate of 0.025 h-1, half-life of 28 h-1 and 2-isopropyl-6-methyl-4-pyrimidinol (IMHP) production of 0.143 mg L h-1. Rapid diazinon hydrolysis to IMHP was associated with a decrease in the pH of the medium as a consequence of microbial glucose metabolism and organic acid exudation. Moreover, the SMC of Streptomyces was able to remove IMHP. This work constitutes a new, if not the only, report on diazinon degradation by mixed cultures of Streptomyces spp. Given the high levels of diazinon removal, the SMC formed by four Streptomyces strains has the potential to be used to treat the diazinon present in environmental matrices.
Fil: Briceño, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Schalchli, H.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Rubilar, O.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Tortella, G.R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Mutis, A.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Palma, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Diez, M.C.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Materia
Mixed Cultures
Actinobacteria
Degradation
Diazinon
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36775
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36775
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed cultureBriceño, G.Schalchli, H.Rubilar, O.Tortella, G.R.Mutis, A.Benimeli, Claudia SusanaPalma, G.Diez, M.C.Mixed CulturesActinobacteriaDegradationDiazinonhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Actinobacteria identified as Streptomyces spp. were evaluated for their ability to remove diazinon as the only carbon source from a liquid medium. Single cultures of Streptomyces strains were exposed to diazinon at a concentration of 50 mg L-1. After 96 h incubation, six of the eight cultures grew and five strains showed an increase in their total protein concentrations and changes in their protein profile. Up to 32% of the diazinon was removed by the single Streptomyces cultures. A compatibility assay showed that the different Streptomyces species were not antagonistic. Twenty-six mixed cultures were then prepared. Diazinon removal was increased when mixed cultures were used, and maximum diazinon removal of 62% was observed when the Streptomyces spp. strains AC5, AC9, GA11 and ISP13 were mixed; this was defined as the selected mixed culture (SMC). Diazinon removal was positively influenced by the addition of glucose into the liquid medium. Our study showed a diazinon degradation rate of 0.025 h-1, half-life of 28 h-1 and 2-isopropyl-6-methyl-4-pyrimidinol (IMHP) production of 0.143 mg L h-1. Rapid diazinon hydrolysis to IMHP was associated with a decrease in the pH of the medium as a consequence of microbial glucose metabolism and organic acid exudation. Moreover, the SMC of Streptomyces was able to remove IMHP. This work constitutes a new, if not the only, report on diazinon degradation by mixed cultures of Streptomyces spp. Given the high levels of diazinon removal, the SMC formed by four Streptomyces strains has the potential to be used to treat the diazinon present in environmental matrices.Fil: Briceño, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Schalchli, H.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Rubilar, O.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Tortella, G.R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Mutis, A.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Palma, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChileFil: Diez, M.C.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; ChilePergamon-Elsevier Science Ltd.2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36775Briceño, G.; Schalchli, H.; Rubilar, O.; Tortella, G.R.; Mutis, A.; et al.; Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture; Pergamon-Elsevier Science Ltd.; Chemosphere; 156; 8-2016; 195-2030045-6535CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2016.04.118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:21Zoai:ri.conicet.gov.ar:11336/36775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:21.514CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
title Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
spellingShingle Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
Briceño, G.
Mixed Cultures
Actinobacteria
Degradation
Diazinon
title_short Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
title_full Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
title_fullStr Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
title_full_unstemmed Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
title_sort Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture
dc.creator.none.fl_str_mv Briceño, G.
Schalchli, H.
Rubilar, O.
Tortella, G.R.
Mutis, A.
Benimeli, Claudia Susana
Palma, G.
Diez, M.C.
author Briceño, G.
author_facet Briceño, G.
Schalchli, H.
Rubilar, O.
Tortella, G.R.
Mutis, A.
Benimeli, Claudia Susana
Palma, G.
Diez, M.C.
author_role author
author2 Schalchli, H.
Rubilar, O.
Tortella, G.R.
Mutis, A.
Benimeli, Claudia Susana
Palma, G.
Diez, M.C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mixed Cultures
Actinobacteria
Degradation
Diazinon
topic Mixed Cultures
Actinobacteria
Degradation
Diazinon
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Actinobacteria identified as Streptomyces spp. were evaluated for their ability to remove diazinon as the only carbon source from a liquid medium. Single cultures of Streptomyces strains were exposed to diazinon at a concentration of 50 mg L-1. After 96 h incubation, six of the eight cultures grew and five strains showed an increase in their total protein concentrations and changes in their protein profile. Up to 32% of the diazinon was removed by the single Streptomyces cultures. A compatibility assay showed that the different Streptomyces species were not antagonistic. Twenty-six mixed cultures were then prepared. Diazinon removal was increased when mixed cultures were used, and maximum diazinon removal of 62% was observed when the Streptomyces spp. strains AC5, AC9, GA11 and ISP13 were mixed; this was defined as the selected mixed culture (SMC). Diazinon removal was positively influenced by the addition of glucose into the liquid medium. Our study showed a diazinon degradation rate of 0.025 h-1, half-life of 28 h-1 and 2-isopropyl-6-methyl-4-pyrimidinol (IMHP) production of 0.143 mg L h-1. Rapid diazinon hydrolysis to IMHP was associated with a decrease in the pH of the medium as a consequence of microbial glucose metabolism and organic acid exudation. Moreover, the SMC of Streptomyces was able to remove IMHP. This work constitutes a new, if not the only, report on diazinon degradation by mixed cultures of Streptomyces spp. Given the high levels of diazinon removal, the SMC formed by four Streptomyces strains has the potential to be used to treat the diazinon present in environmental matrices.
Fil: Briceño, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Schalchli, H.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Rubilar, O.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Tortella, G.R.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Mutis, A.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Palma, G.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
Fil: Diez, M.C.. Universidad de La Frontera. Núcleo Científico y Tecnológico en Recursos Naturales; Chile
description Actinobacteria identified as Streptomyces spp. were evaluated for their ability to remove diazinon as the only carbon source from a liquid medium. Single cultures of Streptomyces strains were exposed to diazinon at a concentration of 50 mg L-1. After 96 h incubation, six of the eight cultures grew and five strains showed an increase in their total protein concentrations and changes in their protein profile. Up to 32% of the diazinon was removed by the single Streptomyces cultures. A compatibility assay showed that the different Streptomyces species were not antagonistic. Twenty-six mixed cultures were then prepared. Diazinon removal was increased when mixed cultures were used, and maximum diazinon removal of 62% was observed when the Streptomyces spp. strains AC5, AC9, GA11 and ISP13 were mixed; this was defined as the selected mixed culture (SMC). Diazinon removal was positively influenced by the addition of glucose into the liquid medium. Our study showed a diazinon degradation rate of 0.025 h-1, half-life of 28 h-1 and 2-isopropyl-6-methyl-4-pyrimidinol (IMHP) production of 0.143 mg L h-1. Rapid diazinon hydrolysis to IMHP was associated with a decrease in the pH of the medium as a consequence of microbial glucose metabolism and organic acid exudation. Moreover, the SMC of Streptomyces was able to remove IMHP. This work constitutes a new, if not the only, report on diazinon degradation by mixed cultures of Streptomyces spp. Given the high levels of diazinon removal, the SMC formed by four Streptomyces strains has the potential to be used to treat the diazinon present in environmental matrices.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36775
Briceño, G.; Schalchli, H.; Rubilar, O.; Tortella, G.R.; Mutis, A.; et al.; Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture; Pergamon-Elsevier Science Ltd.; Chemosphere; 156; 8-2016; 195-203
0045-6535
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36775
identifier_str_mv Briceño, G.; Schalchli, H.; Rubilar, O.; Tortella, G.R.; Mutis, A.; et al.; Increased diazinon hydrolysis to 2-isopropyl-6-methyl-4-pyrimidinol in liquid medium by a specific Streptomyces mixed culture; Pergamon-Elsevier Science Ltd.; Chemosphere; 156; 8-2016; 195-203
0045-6535
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2016.04.118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd.
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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