Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil

Autores
Briceño, Gabriela; Fuentes, María Soledad; Rubilar, Olga; Jorquera, Milko; Tortella, Gonzalo; Palma, Graciela; Amoroso, Maria Julia del R.; Diez, María C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
From an agricultural soil that had received continuous applications of organophosphorus pesticides, 30 actinobacteria strains were isolated. Two strains, identified as Streptomyces sp. AC1-6 and Streptomyces sp. ISP4, were selected because of their tolerance to diazinon and based on the relationship between diazinon removal and microbial growth. In liquid medium with diazinon at concentrations of 25 and 50mgL-1, both strains were able to remove approximately 40-50% and 70-90% of the initial diazinon after 24 and 96h of incubation, respectively. This diazinon removal was accompanied by microbial growth of the strains, an initial pH decrease, and glucose consumption in the liquid medium. Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp. AC1-6 immobilized on alginate beads revealed that the immobilized cells exhibited a 60% higher diazinon removal compared with the free cells. The reusability of the encapsulated biomass was confirmed, and a diazinon removal rate of more than 50% was obtained after the second batch. This work constitutes one of the few reports that describe Streptomyces strains as diazinon degraders. Given the high diazinon removal found, the streptomycetes exhibit suitable potential as diazinon-degrading actinobacteria for elimination of diazinon from liquid residues.
Fil: Briceño, Gabriela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rubilar, Olga. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Jorquera, Milko. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Tortella, Gonzalo. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Palma, Graciela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Amoroso, Maria Julia del R.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Diez, María C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Materia
CA-ALGINATE BEADS
DIAZINON
FREE CELLS
IMMOBILIZED CELLS
STREPTOMYCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1792
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1792
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soilBriceño, GabrielaFuentes, María SoledadRubilar, OlgaJorquera, MilkoTortella, GonzaloPalma, GracielaAmoroso, Maria Julia del R.Diez, María C.CA-ALGINATE BEADSDIAZINONFREE CELLSIMMOBILIZED CELLSSTREPTOMYCEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2From an agricultural soil that had received continuous applications of organophosphorus pesticides, 30 actinobacteria strains were isolated. Two strains, identified as Streptomyces sp. AC1-6 and Streptomyces sp. ISP4, were selected because of their tolerance to diazinon and based on the relationship between diazinon removal and microbial growth. In liquid medium with diazinon at concentrations of 25 and 50mgL-1, both strains were able to remove approximately 40-50% and 70-90% of the initial diazinon after 24 and 96h of incubation, respectively. This diazinon removal was accompanied by microbial growth of the strains, an initial pH decrease, and glucose consumption in the liquid medium. Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp. AC1-6 immobilized on alginate beads revealed that the immobilized cells exhibited a 60% higher diazinon removal compared with the free cells. The reusability of the encapsulated biomass was confirmed, and a diazinon removal rate of more than 50% was obtained after the second batch. This work constitutes one of the few reports that describe Streptomyces strains as diazinon degraders. Given the high diazinon removal found, the streptomycetes exhibit suitable potential as diazinon-degrading actinobacteria for elimination of diazinon from liquid residues.Fil: Briceño, Gabriela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rubilar, Olga. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Fil: Jorquera, Milko. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Fil: Tortella, Gonzalo. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Fil: Palma, Graciela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Fil: Amoroso, Maria Julia del R.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Diez, María C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;Wiley VCH Verlag2013-12-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1792Briceño, Gabriela; Fuentes, María Soledad; Rubilar, Olga; Jorquera, Milko; Tortella, Gonzalo; et al.; Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil; Wiley VCH Verlag; Journal of Basic Microbiology; 55; 3; 11-12-2013; 293-3020233-111Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.201300576info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:17Zoai:ri.conicet.gov.ar:11336/1792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:17.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
title Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
spellingShingle Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
Briceño, Gabriela
CA-ALGINATE BEADS
DIAZINON
FREE CELLS
IMMOBILIZED CELLS
STREPTOMYCES
title_short Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
title_full Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
title_fullStr Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
title_full_unstemmed Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
title_sort Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
dc.creator.none.fl_str_mv Briceño, Gabriela
Fuentes, María Soledad
Rubilar, Olga
Jorquera, Milko
Tortella, Gonzalo
Palma, Graciela
Amoroso, Maria Julia del R.
Diez, María C.
author Briceño, Gabriela
author_facet Briceño, Gabriela
Fuentes, María Soledad
Rubilar, Olga
Jorquera, Milko
Tortella, Gonzalo
Palma, Graciela
Amoroso, Maria Julia del R.
Diez, María C.
author_role author
author2 Fuentes, María Soledad
Rubilar, Olga
Jorquera, Milko
Tortella, Gonzalo
Palma, Graciela
Amoroso, Maria Julia del R.
Diez, María C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CA-ALGINATE BEADS
DIAZINON
FREE CELLS
IMMOBILIZED CELLS
STREPTOMYCES
topic CA-ALGINATE BEADS
DIAZINON
FREE CELLS
IMMOBILIZED CELLS
STREPTOMYCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv From an agricultural soil that had received continuous applications of organophosphorus pesticides, 30 actinobacteria strains were isolated. Two strains, identified as Streptomyces sp. AC1-6 and Streptomyces sp. ISP4, were selected because of their tolerance to diazinon and based on the relationship between diazinon removal and microbial growth. In liquid medium with diazinon at concentrations of 25 and 50mgL-1, both strains were able to remove approximately 40-50% and 70-90% of the initial diazinon after 24 and 96h of incubation, respectively. This diazinon removal was accompanied by microbial growth of the strains, an initial pH decrease, and glucose consumption in the liquid medium. Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp. AC1-6 immobilized on alginate beads revealed that the immobilized cells exhibited a 60% higher diazinon removal compared with the free cells. The reusability of the encapsulated biomass was confirmed, and a diazinon removal rate of more than 50% was obtained after the second batch. This work constitutes one of the few reports that describe Streptomyces strains as diazinon degraders. Given the high diazinon removal found, the streptomycetes exhibit suitable potential as diazinon-degrading actinobacteria for elimination of diazinon from liquid residues.
Fil: Briceño, Gabriela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rubilar, Olga. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Jorquera, Milko. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Tortella, Gonzalo. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Palma, Graciela. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
Fil: Amoroso, Maria Julia del R.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Diez, María C.. Universidad de la Frontera. Nucleo Cientifico y Tecnológico en Recursos Naturales; Chile;
description From an agricultural soil that had received continuous applications of organophosphorus pesticides, 30 actinobacteria strains were isolated. Two strains, identified as Streptomyces sp. AC1-6 and Streptomyces sp. ISP4, were selected because of their tolerance to diazinon and based on the relationship between diazinon removal and microbial growth. In liquid medium with diazinon at concentrations of 25 and 50mgL-1, both strains were able to remove approximately 40-50% and 70-90% of the initial diazinon after 24 and 96h of incubation, respectively. This diazinon removal was accompanied by microbial growth of the strains, an initial pH decrease, and glucose consumption in the liquid medium. Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp. AC1-6 immobilized on alginate beads revealed that the immobilized cells exhibited a 60% higher diazinon removal compared with the free cells. The reusability of the encapsulated biomass was confirmed, and a diazinon removal rate of more than 50% was obtained after the second batch. This work constitutes one of the few reports that describe Streptomyces strains as diazinon degraders. Given the high diazinon removal found, the streptomycetes exhibit suitable potential as diazinon-degrading actinobacteria for elimination of diazinon from liquid residues.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1792
Briceño, Gabriela; Fuentes, María Soledad; Rubilar, Olga; Jorquera, Milko; Tortella, Gonzalo; et al.; Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil; Wiley VCH Verlag; Journal of Basic Microbiology; 55; 3; 11-12-2013; 293-302
0233-111X
url http://hdl.handle.net/11336/1792
identifier_str_mv Briceño, Gabriela; Fuentes, María Soledad; Rubilar, Olga; Jorquera, Milko; Tortella, Gonzalo; et al.; Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil; Wiley VCH Verlag; Journal of Basic Microbiology; 55; 3; 11-12-2013; 293-302
0233-111X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.201300576
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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