The interactome of Streptococcus pneumoniae and its bacteriophages show highly specific patterns of interactions among bacteria and their phages

• Autores: Rachelle, Mariano; Wuchty, Stefan; Vizoso Pinto, María Guadalupe; Häuser, Roman; Uetz, Peter
• Año de publicación: 2016
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: INTERACTOME; PHAGE; STREPTOCOCCUS; YEAST TWO HYBRID
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Although an abundance of bacteriophages exists, little is known about interactions between their proteins and those of their bacterial hosts. Here, we experimentally determined the phage-host interactomes of the phages Dp-1 and Cp-1 and their underlying protein interaction network in the host Streptococcus pneumoniae. We compared our results to the interaction patterns of E. coli phages lambda and T7. Dp-1 and Cp-1 target highly connected host proteins, occupy central network positions, and reach many protein clusters through the interactions of their targets. In turn, lambda and T7 targets cluster to conserved and essential proteins in E. coli, while such patterns were largely absent in S. pneumoniae. Furthermore, targets in E. coli were mutually strongly intertwined, while targets of Dp-1 and Cp-1 were strongly connected through essential and orthologous proteins in their immediate network vicinity. In both phage-host systems, the impact of phages on their protein targets appears to extend from their network neighbors, since proteins that interact with phage targets were located in central network positions, have a strong topologically disruptive effect and touch complexes with high functional heterogeneity. Such observations suggest that the phages, biological impact is accomplished through a surprisingly limited topological reach of their targets.
Fil: Rachelle, Mariano. University of Miami; Estados Unidos. Harvard University; Estados Unidos
Fil: Wuchty, Stefan. University of Miami; Estados Unidos
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Ludwig Maximilians Universitat. Max Von Pettenkofer Institute; Alemania
Fil: Häuser, Roman. German Cancer Research Center; Alemania
Fil: Uetz, Peter. Virginia Commonwealth University; Estados Unidos