Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins

Autores
Jaramillo Ortiz, José Manuel; del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Molinari, Maria Paula; Gravisaco, María José; Calamante, Gabriela; Wilkowky, Silvina Elizabeth
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigenwas obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immu-nizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominantB and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were eval-uated in mice to compare the immunogenicity induced by different immunization schemes. The bestvaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinantvirus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree ofactivation of IFN+CD4+and CD8+specific T cells. This is the first report in which a novel vaccine can-didate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boostregime, optimizing the immune response necessary for protection against bovine babesiosis.
Fil: Jaramillo Ortiz, José Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Wilkowky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
Babesia Bovis
Multiantígeno
Virus Vaccinia Ankara Modificado
Proteínas Recombinantes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4109
Acceder
id CONICETDig_34ea8f7de87d947edd124949a889859c
oai_identifier_str oai:ri.conicet.gov.ar:11336/4109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteinsJaramillo Ortiz, José Manueldel Medico Zajac, Maria PaulaZanetti, Flavia AdrianaMolinari, Maria PaulaGravisaco, María JoséCalamante, GabrielaWilkowky, Silvina ElizabethBabesia BovisMultiantígenoVirus Vaccinia Ankara ModificadoProteínas Recombinanteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigenwas obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immu-nizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominantB and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were eval-uated in mice to compare the immunogenicity induced by different immunization schemes. The bestvaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinantvirus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree ofactivation of IFN+CD4+and CD8+specific T cells. This is the first report in which a novel vaccine can-didate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boostregime, optimizing the immune response necessary for protection against bovine babesiosis.Fil: Jaramillo Ortiz, José Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Wilkowky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2014-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4109Jaramillo Ortiz, José Manuel; del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Molinari, Maria Paula; Gravisaco, María José; et al.; Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins; Elsevier; Vaccine; 32; 36; 23-6-2014; 4625-46320264-410Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0264410X14008767info:eu-repo/semantics/altIdentifier/issn/0264-410Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2014.06.075info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:33Zoai:ri.conicet.gov.ar:11336/4109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:33.6CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
title Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
spellingShingle Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
Jaramillo Ortiz, José Manuel
Babesia Bovis
Multiantígeno
Virus Vaccinia Ankara Modificado
Proteínas Recombinantes
title_short Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
title_full Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
title_fullStr Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
title_full_unstemmed Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
title_sort Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins
dc.creator.none.fl_str_mv Jaramillo Ortiz, José Manuel
del Medico Zajac, Maria Paula
Zanetti, Flavia Adriana
Molinari, Maria Paula
Gravisaco, María José
Calamante, Gabriela
Wilkowky, Silvina Elizabeth
author Jaramillo Ortiz, José Manuel
author_facet Jaramillo Ortiz, José Manuel
del Medico Zajac, Maria Paula
Zanetti, Flavia Adriana
Molinari, Maria Paula
Gravisaco, María José
Calamante, Gabriela
Wilkowky, Silvina Elizabeth
author_role author
author2 del Medico Zajac, Maria Paula
Zanetti, Flavia Adriana
Molinari, Maria Paula
Gravisaco, María José
Calamante, Gabriela
Wilkowky, Silvina Elizabeth
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia Bovis
Multiantígeno
Virus Vaccinia Ankara Modificado
Proteínas Recombinantes
topic Babesia Bovis
Multiantígeno
Virus Vaccinia Ankara Modificado
Proteínas Recombinantes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigenwas obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immu-nizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominantB and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were eval-uated in mice to compare the immunogenicity induced by different immunization schemes. The bestvaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinantvirus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree ofactivation of IFN+CD4+and CD8+specific T cells. This is the first report in which a novel vaccine can-didate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boostregime, optimizing the immune response necessary for protection against bovine babesiosis.
Fil: Jaramillo Ortiz, José Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Wilkowky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description In this study, a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigenwas obtained and evaluated as a candidate vaccine in homologous and heterologous prime-boost immu-nizations with a recombinant protein cocktail. The chimeric multi-antigen comprises immunodominantB and T cell regions of three Babesia bovis proteins. Humoral and cellular immune responses were eval-uated in mice to compare the immunogenicity induced by different immunization schemes. The bestvaccination scheme was achieved with a prime of protein cocktail and a boost with the recombinantvirus. This scheme induced high level of specific IgG antibodies and secreted IFN and a high degree ofactivation of IFN+CD4+and CD8+specific T cells. This is the first report in which a novel vaccine can-didate was constructed based on a rationally designed multi-antigen and evaluated in a prime-boostregime, optimizing the immune response necessary for protection against bovine babesiosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4109
Jaramillo Ortiz, José Manuel; del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Molinari, Maria Paula; Gravisaco, María José; et al.; Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins; Elsevier; Vaccine; 32; 36; 23-6-2014; 4625-4632
0264-410X
url http://hdl.handle.net/11336/4109
identifier_str_mv Jaramillo Ortiz, José Manuel; del Medico Zajac, Maria Paula; Zanetti, Flavia Adriana; Molinari, Maria Paula; Gravisaco, María José; et al.; Vaccine strategies against Babesia bovis based on prime-boost immunizations in mice with modified vaccinia Ankara vector and recombinant proteins; Elsevier; Vaccine; 32; 36; 23-6-2014; 4625-4632
0264-410X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0264410X14008767
info:eu-repo/semantics/altIdentifier/issn/0264-410X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2014.06.075
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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