Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigge...

Autores
Jaramillo Ortiz, Jose Manuel; Paoletta, Martina; Gravisaco, María José; Lopez Arias, Ludmila Sol; Montenegro, Valeria Noely; De La Fourniere, Sofia; Valenzano, Magali Nicole; Guillemi, Eliana Carolina; Valentini, Beatriz Susana; Echaide, Ignacio Eduardo; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by professional antigen-presenting cells. Secretion of Th1 cytokines by CD4+ T cell is also very important for isotype switching to IgG2, the best opsonising antibody isotype in cattle, to target extracellular parasites and parasite antigens displayed at the erythrocyte surface. In the field of vaccinology, heterologous prime-boost schemes combining protein-adjuvant formulations with a modified vaccinia Ankara vector expressing the same antigen have demonstrated the induction of both humoral and cellular immune responses. It has been previously demonstrated that MVA-infected dendritic cells can present antigens in the context of MHC II and activate CD4+ T cell. These results support the use of the MVA viral vector for a pathogen like Babesia bovis, which only resides within erythrocytes. In this study, 13-15-months-old Holstein-Friesian steers were immunised with a subunit vaccine as a prime and a modified vaccinia Ankara vector as a boost, both expressing a chimeric multi-antigen (rMABbo - rMVA). This antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: merozoite surface antigen - 2c (MSA - 2c), rhoptry associated protein 1 (RAP - 1) and heat shock protein 20 (HSP20). Responses were compared with the Babesia bovis live attenuated vaccine used in Argentina (R1A). Eleven weeks after the first immunisation, all bovines were challenged by the inoculation of a virulent B. bovis strain. All groups were monitored daily for hyperthermia and reduction of packed cell volume. Both the rMABbo - rMVA and R1A vaccinated animals developed high titters of total IgG antibodies and an antigen-specific Th1 cellular response before and after challenge. However, all rMABbo - rMVA steers showed clinical signs of disease upon challenge. Only the R1A live vaccine group developed an immune response associated with in vitro neutralising antibodies at a level that significantly inhibited the parasite invasion. The lack of protection observed with this recombinant formulation indicates the need to perform further basic and clinical studies in the bovine model in order to achieve the desired effectiveness. This is the first report in which a novel vaccine candidate against Babesia bovis was constructed based on a recombinant and rationally designed viral vector and evaluated in the biological model of the disease.
Instituto de Biotecnología
Fil: Jaramillo Ortiz, Jose Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valenzano, Magalí Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Ticks and tick-borne diseases 10 (6) : 101270. (Octubre 2019)
Materia
Babesia bovis
Antígenos
Anticuerpos
Antigens
Antibodies
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6630
Acceder
id INTADig_e8f8907c8e8a91bc4c6461ee8e65e355
oai_identifier_str oai:localhost:20.500.12123/6630
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protectionJaramillo Ortiz, Jose ManuelPaoletta, MartinaGravisaco, María JoséLopez Arias, Ludmila SolMontenegro, Valeria NoelyDe La Fourniere, SofiaValenzano, Magali NicoleGuillemi, Eliana CarolinaValentini, Beatriz SusanaEchaide, Ignacio EduardoFarber, Marisa DianaWilkowsky, Silvina ElizabethBabesia bovisAntígenosAnticuerposAntigensAntibodiesProtection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by professional antigen-presenting cells. Secretion of Th1 cytokines by CD4+ T cell is also very important for isotype switching to IgG2, the best opsonising antibody isotype in cattle, to target extracellular parasites and parasite antigens displayed at the erythrocyte surface. In the field of vaccinology, heterologous prime-boost schemes combining protein-adjuvant formulations with a modified vaccinia Ankara vector expressing the same antigen have demonstrated the induction of both humoral and cellular immune responses. It has been previously demonstrated that MVA-infected dendritic cells can present antigens in the context of MHC II and activate CD4+ T cell. These results support the use of the MVA viral vector for a pathogen like Babesia bovis, which only resides within erythrocytes. In this study, 13-15-months-old Holstein-Friesian steers were immunised with a subunit vaccine as a prime and a modified vaccinia Ankara vector as a boost, both expressing a chimeric multi-antigen (rMABbo - rMVA). This antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: merozoite surface antigen - 2c (MSA - 2c), rhoptry associated protein 1 (RAP - 1) and heat shock protein 20 (HSP20). Responses were compared with the Babesia bovis live attenuated vaccine used in Argentina (R1A). Eleven weeks after the first immunisation, all bovines were challenged by the inoculation of a virulent B. bovis strain. All groups were monitored daily for hyperthermia and reduction of packed cell volume. Both the rMABbo - rMVA and R1A vaccinated animals developed high titters of total IgG antibodies and an antigen-specific Th1 cellular response before and after challenge. However, all rMABbo - rMVA steers showed clinical signs of disease upon challenge. Only the R1A live vaccine group developed an immune response associated with in vitro neutralising antibodies at a level that significantly inhibited the parasite invasion. The lack of protection observed with this recombinant formulation indicates the need to perform further basic and clinical studies in the bovine model in order to achieve the desired effectiveness. This is the first report in which a novel vaccine candidate against Babesia bovis was constructed based on a recombinant and rationally designed viral vector and evaluated in the biological model of the disease.Instituto de BiotecnologíaFil: Jaramillo Ortiz, Jose Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valenzano, Magalí Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2020-01-07T17:37:03Z2020-01-07T17:37:03Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6630https://www.sciencedirect.com/science/article/pii/S1877959X19300901?via%3Dihub1877-959Xhttps://doi.org/10.1016/j.ttbdis.2019.101270Ticks and tick-borne diseases 10 (6) : 101270. (Octubre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:19Zoai:localhost:20.500.12123/6630instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:19.93INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
title Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
spellingShingle Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
Jaramillo Ortiz, Jose Manuel
Babesia bovis
Antígenos
Anticuerpos
Antigens
Antibodies
title_short Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
title_full Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
title_fullStr Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
title_full_unstemmed Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
title_sort Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
dc.creator.none.fl_str_mv Jaramillo Ortiz, Jose Manuel
Paoletta, Martina
Gravisaco, María José
Lopez Arias, Ludmila Sol
Montenegro, Valeria Noely
De La Fourniere, Sofia
Valenzano, Magali Nicole
Guillemi, Eliana Carolina
Valentini, Beatriz Susana
Echaide, Ignacio Eduardo
Farber, Marisa Diana
Wilkowsky, Silvina Elizabeth
author Jaramillo Ortiz, Jose Manuel
author_facet Jaramillo Ortiz, Jose Manuel
Paoletta, Martina
Gravisaco, María José
Lopez Arias, Ludmila Sol
Montenegro, Valeria Noely
De La Fourniere, Sofia
Valenzano, Magali Nicole
Guillemi, Eliana Carolina
Valentini, Beatriz Susana
Echaide, Ignacio Eduardo
Farber, Marisa Diana
Wilkowsky, Silvina Elizabeth
author_role author
author2 Paoletta, Martina
Gravisaco, María José
Lopez Arias, Ludmila Sol
Montenegro, Valeria Noely
De La Fourniere, Sofia
Valenzano, Magali Nicole
Guillemi, Eliana Carolina
Valentini, Beatriz Susana
Echaide, Ignacio Eduardo
Farber, Marisa Diana
Wilkowsky, Silvina Elizabeth
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia bovis
Antígenos
Anticuerpos
Antigens
Antibodies
topic Babesia bovis
Antígenos
Anticuerpos
Antigens
Antibodies
dc.description.none.fl_txt_mv Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by professional antigen-presenting cells. Secretion of Th1 cytokines by CD4+ T cell is also very important for isotype switching to IgG2, the best opsonising antibody isotype in cattle, to target extracellular parasites and parasite antigens displayed at the erythrocyte surface. In the field of vaccinology, heterologous prime-boost schemes combining protein-adjuvant formulations with a modified vaccinia Ankara vector expressing the same antigen have demonstrated the induction of both humoral and cellular immune responses. It has been previously demonstrated that MVA-infected dendritic cells can present antigens in the context of MHC II and activate CD4+ T cell. These results support the use of the MVA viral vector for a pathogen like Babesia bovis, which only resides within erythrocytes. In this study, 13-15-months-old Holstein-Friesian steers were immunised with a subunit vaccine as a prime and a modified vaccinia Ankara vector as a boost, both expressing a chimeric multi-antigen (rMABbo - rMVA). This antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: merozoite surface antigen - 2c (MSA - 2c), rhoptry associated protein 1 (RAP - 1) and heat shock protein 20 (HSP20). Responses were compared with the Babesia bovis live attenuated vaccine used in Argentina (R1A). Eleven weeks after the first immunisation, all bovines were challenged by the inoculation of a virulent B. bovis strain. All groups were monitored daily for hyperthermia and reduction of packed cell volume. Both the rMABbo - rMVA and R1A vaccinated animals developed high titters of total IgG antibodies and an antigen-specific Th1 cellular response before and after challenge. However, all rMABbo - rMVA steers showed clinical signs of disease upon challenge. Only the R1A live vaccine group developed an immune response associated with in vitro neutralising antibodies at a level that significantly inhibited the parasite invasion. The lack of protection observed with this recombinant formulation indicates the need to perform further basic and clinical studies in the bovine model in order to achieve the desired effectiveness. This is the first report in which a novel vaccine candidate against Babesia bovis was constructed based on a recombinant and rationally designed viral vector and evaluated in the biological model of the disease.
Instituto de Biotecnología
Fil: Jaramillo Ortiz, Jose Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valenzano, Magalí Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Laboratorio de Hemoparásitos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by professional antigen-presenting cells. Secretion of Th1 cytokines by CD4+ T cell is also very important for isotype switching to IgG2, the best opsonising antibody isotype in cattle, to target extracellular parasites and parasite antigens displayed at the erythrocyte surface. In the field of vaccinology, heterologous prime-boost schemes combining protein-adjuvant formulations with a modified vaccinia Ankara vector expressing the same antigen have demonstrated the induction of both humoral and cellular immune responses. It has been previously demonstrated that MVA-infected dendritic cells can present antigens in the context of MHC II and activate CD4+ T cell. These results support the use of the MVA viral vector for a pathogen like Babesia bovis, which only resides within erythrocytes. In this study, 13-15-months-old Holstein-Friesian steers were immunised with a subunit vaccine as a prime and a modified vaccinia Ankara vector as a boost, both expressing a chimeric multi-antigen (rMABbo - rMVA). This antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: merozoite surface antigen - 2c (MSA - 2c), rhoptry associated protein 1 (RAP - 1) and heat shock protein 20 (HSP20). Responses were compared with the Babesia bovis live attenuated vaccine used in Argentina (R1A). Eleven weeks after the first immunisation, all bovines were challenged by the inoculation of a virulent B. bovis strain. All groups were monitored daily for hyperthermia and reduction of packed cell volume. Both the rMABbo - rMVA and R1A vaccinated animals developed high titters of total IgG antibodies and an antigen-specific Th1 cellular response before and after challenge. However, all rMABbo - rMVA steers showed clinical signs of disease upon challenge. Only the R1A live vaccine group developed an immune response associated with in vitro neutralising antibodies at a level that significantly inhibited the parasite invasion. The lack of protection observed with this recombinant formulation indicates the need to perform further basic and clinical studies in the bovine model in order to achieve the desired effectiveness. This is the first report in which a novel vaccine candidate against Babesia bovis was constructed based on a recombinant and rationally designed viral vector and evaluated in the biological model of the disease.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
2020-01-07T17:37:03Z
2020-01-07T17:37:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6630
https://www.sciencedirect.com/science/article/pii/S1877959X19300901?via%3Dihub
1877-959X
https://doi.org/10.1016/j.ttbdis.2019.101270
url http://hdl.handle.net/20.500.12123/6630
https://www.sciencedirect.com/science/article/pii/S1877959X19300901?via%3Dihub
https://doi.org/10.1016/j.ttbdis.2019.101270
identifier_str_mv 1877-959X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ticks and tick-borne diseases 10 (6) : 101270. (Octubre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341375417253888
score 12.623145

Publicaciones similares