Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix

Autores
Ramírez, N.; Guerra, Fernando Andres; Camporeale, Gabriela; Quintana, S.; Diaz, L. B.; Cuneo, N.; Villacorta Hidalgo, J.; Tatti, S. A.; Alonso, Leonardo Gabriel; Borkosky, Silvia Susana; de Prat Gay, Gonzalo; Palaoro, L.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Continuous production of the E7 protein from different types of high risk human papilloma virus (HPV) is required for progression of malignancy. We developed antibodies against HPV type 16 E7 and E2 proteins to evaluate their utility as markers for diagnosis during early stages of cervical cancer. Forty biopsies from uterine cervices were diagnosed as low grade intraepithelial lesion (LSIL), high grade intraepithelial lesion (HSIL), squamous carcinoma (SC), in situ adenocarcinoma (ISA) and invasive adenocarcinoma (AC), all of which were infected with HPV 16. Immunohistochemistry was used to investigate the expressions of E7 and E2 (both from HPV 16) and p16. P16 was expressed in eight of 12 LSILs, in all HSILs, in 16 of 18 SC and in all ACs. E2 was expressed in six of 12 LSILs. E7 was positive in eight of 12 LSILs and in all HSIL and carcinomas. The expressions of E2 and E7 of HPV16 related to p16 expression confirmed the value of the viral oncogenic proteins as complementary to histology and support the carcinogenic model of the uterine cervix, because HPVDNA integration into cellular DNA implies the destruction of the gene encoding E2, which suppresses the expression of the E6 and E7 oncoproteins. E2 from HPV16 could be marker for LSILs, while E7 could be a marker for progression of LSILs to HSILs in patients infected by HPV16, because viral typing has little positive predictive value.
Fil: Ramírez, N.. Furtwangen University; Alemania
Fil: Guerra, Fernando Andres. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Camporeale, Gabriela. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Quintana, S.. Instituto Fares Taie. Laboratorio de Biología Molecular; Argentina
Fil: Diaz, L. B.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Cuneo, N.. Hospital de Oncología María Curie; Argentina
Fil: Villacorta Hidalgo, J.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Tatti, S. A.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Alonso, Leonardo Gabriel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Borkosky, Silvia Susana. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: de Prat Gay, Gonzalo. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Palaoro, L.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Materia
Adenocarcinoma
Biomarkers
Papillomavirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14464
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14464
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervixRamírez, N.Guerra, Fernando AndresCamporeale, GabrielaQuintana, S.Diaz, L. B.Cuneo, N.Villacorta Hidalgo, J.Tatti, S. A.Alonso, Leonardo GabrielBorkosky, Silvia Susanade Prat Gay, GonzaloPalaoro, L.AdenocarcinomaBiomarkersPapillomavirushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Continuous production of the E7 protein from different types of high risk human papilloma virus (HPV) is required for progression of malignancy. We developed antibodies against HPV type 16 E7 and E2 proteins to evaluate their utility as markers for diagnosis during early stages of cervical cancer. Forty biopsies from uterine cervices were diagnosed as low grade intraepithelial lesion (LSIL), high grade intraepithelial lesion (HSIL), squamous carcinoma (SC), in situ adenocarcinoma (ISA) and invasive adenocarcinoma (AC), all of which were infected with HPV 16. Immunohistochemistry was used to investigate the expressions of E7 and E2 (both from HPV 16) and p16. P16 was expressed in eight of 12 LSILs, in all HSILs, in 16 of 18 SC and in all ACs. E2 was expressed in six of 12 LSILs. E7 was positive in eight of 12 LSILs and in all HSIL and carcinomas. The expressions of E2 and E7 of HPV16 related to p16 expression confirmed the value of the viral oncogenic proteins as complementary to histology and support the carcinogenic model of the uterine cervix, because HPVDNA integration into cellular DNA implies the destruction of the gene encoding E2, which suppresses the expression of the E6 and E7 oncoproteins. E2 from HPV16 could be marker for LSILs, while E7 could be a marker for progression of LSILs to HSILs in patients infected by HPV16, because viral typing has little positive predictive value.Fil: Ramírez, N.. Furtwangen University; AlemaniaFil: Guerra, Fernando Andres. Universidad de Buenos Aires. Hospital de Clínicas; ArgentinaFil: Camporeale, Gabriela. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Quintana, S.. Instituto Fares Taie. Laboratorio de Biología Molecular; ArgentinaFil: Diaz, L. B.. Universidad de Buenos Aires. Hospital de Clínicas; ArgentinaFil: Cuneo, N.. Hospital de Oncología María Curie; ArgentinaFil: Villacorta Hidalgo, J.. Universidad de Buenos Aires. Hospital de Clínicas; ArgentinaFil: Tatti, S. A.. Universidad de Buenos Aires. Hospital de Clínicas; ArgentinaFil: Alonso, Leonardo Gabriel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Borkosky, Silvia Susana. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: de Prat Gay, Gonzalo. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; ArgentinaFil: Palaoro, L.. Universidad de Buenos Aires. Hospital de Clínicas; ArgentinaInforma Healthcare2015-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14464Ramírez, N.; Guerra, Fernando Andres; Camporeale, Gabriela; Quintana, S.; Diaz, L. B.; et al.; Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix; Informa Healthcare; Biotechnic And Histochemistry; 90; 8; 08-6-2015; 573-5801473-7760enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2015.1047794info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2015.1047794info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:55Zoai:ri.conicet.gov.ar:11336/14464instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:55.826CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
title Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
spellingShingle Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
Ramírez, N.
Adenocarcinoma
Biomarkers
Papillomavirus
title_short Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
title_full Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
title_fullStr Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
title_full_unstemmed Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
title_sort Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix
dc.creator.none.fl_str_mv Ramírez, N.
Guerra, Fernando Andres
Camporeale, Gabriela
Quintana, S.
Diaz, L. B.
Cuneo, N.
Villacorta Hidalgo, J.
Tatti, S. A.
Alonso, Leonardo Gabriel
Borkosky, Silvia Susana
de Prat Gay, Gonzalo
Palaoro, L.
author Ramírez, N.
author_facet Ramírez, N.
Guerra, Fernando Andres
Camporeale, Gabriela
Quintana, S.
Diaz, L. B.
Cuneo, N.
Villacorta Hidalgo, J.
Tatti, S. A.
Alonso, Leonardo Gabriel
Borkosky, Silvia Susana
de Prat Gay, Gonzalo
Palaoro, L.
author_role author
author2 Guerra, Fernando Andres
Camporeale, Gabriela
Quintana, S.
Diaz, L. B.
Cuneo, N.
Villacorta Hidalgo, J.
Tatti, S. A.
Alonso, Leonardo Gabriel
Borkosky, Silvia Susana
de Prat Gay, Gonzalo
Palaoro, L.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adenocarcinoma
Biomarkers
Papillomavirus
topic Adenocarcinoma
Biomarkers
Papillomavirus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Continuous production of the E7 protein from different types of high risk human papilloma virus (HPV) is required for progression of malignancy. We developed antibodies against HPV type 16 E7 and E2 proteins to evaluate their utility as markers for diagnosis during early stages of cervical cancer. Forty biopsies from uterine cervices were diagnosed as low grade intraepithelial lesion (LSIL), high grade intraepithelial lesion (HSIL), squamous carcinoma (SC), in situ adenocarcinoma (ISA) and invasive adenocarcinoma (AC), all of which were infected with HPV 16. Immunohistochemistry was used to investigate the expressions of E7 and E2 (both from HPV 16) and p16. P16 was expressed in eight of 12 LSILs, in all HSILs, in 16 of 18 SC and in all ACs. E2 was expressed in six of 12 LSILs. E7 was positive in eight of 12 LSILs and in all HSIL and carcinomas. The expressions of E2 and E7 of HPV16 related to p16 expression confirmed the value of the viral oncogenic proteins as complementary to histology and support the carcinogenic model of the uterine cervix, because HPVDNA integration into cellular DNA implies the destruction of the gene encoding E2, which suppresses the expression of the E6 and E7 oncoproteins. E2 from HPV16 could be marker for LSILs, while E7 could be a marker for progression of LSILs to HSILs in patients infected by HPV16, because viral typing has little positive predictive value.
Fil: Ramírez, N.. Furtwangen University; Alemania
Fil: Guerra, Fernando Andres. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Camporeale, Gabriela. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Quintana, S.. Instituto Fares Taie. Laboratorio de Biología Molecular; Argentina
Fil: Diaz, L. B.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Cuneo, N.. Hospital de Oncología María Curie; Argentina
Fil: Villacorta Hidalgo, J.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Tatti, S. A.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
Fil: Alonso, Leonardo Gabriel. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Borkosky, Silvia Susana. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: de Prat Gay, Gonzalo. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina
Fil: Palaoro, L.. Universidad de Buenos Aires. Hospital de Clínicas; Argentina
description Continuous production of the E7 protein from different types of high risk human papilloma virus (HPV) is required for progression of malignancy. We developed antibodies against HPV type 16 E7 and E2 proteins to evaluate their utility as markers for diagnosis during early stages of cervical cancer. Forty biopsies from uterine cervices were diagnosed as low grade intraepithelial lesion (LSIL), high grade intraepithelial lesion (HSIL), squamous carcinoma (SC), in situ adenocarcinoma (ISA) and invasive adenocarcinoma (AC), all of which were infected with HPV 16. Immunohistochemistry was used to investigate the expressions of E7 and E2 (both from HPV 16) and p16. P16 was expressed in eight of 12 LSILs, in all HSILs, in 16 of 18 SC and in all ACs. E2 was expressed in six of 12 LSILs. E7 was positive in eight of 12 LSILs and in all HSIL and carcinomas. The expressions of E2 and E7 of HPV16 related to p16 expression confirmed the value of the viral oncogenic proteins as complementary to histology and support the carcinogenic model of the uterine cervix, because HPVDNA integration into cellular DNA implies the destruction of the gene encoding E2, which suppresses the expression of the E6 and E7 oncoproteins. E2 from HPV16 could be marker for LSILs, while E7 could be a marker for progression of LSILs to HSILs in patients infected by HPV16, because viral typing has little positive predictive value.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14464
Ramírez, N.; Guerra, Fernando Andres; Camporeale, Gabriela; Quintana, S.; Diaz, L. B.; et al.; Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix; Informa Healthcare; Biotechnic And Histochemistry; 90; 8; 08-6-2015; 573-580
1473-7760
url http://hdl.handle.net/11336/14464
identifier_str_mv Ramírez, N.; Guerra, Fernando Andres; Camporeale, Gabriela; Quintana, S.; Diaz, L. B.; et al.; Expressions of E2 and E7-HPV16 proteins in pre-malignant and malignant lesions of the uterine cervix; Informa Healthcare; Biotechnic And Histochemistry; 90; 8; 08-6-2015; 573-580
1473-7760
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10520295.2015.1047794
info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2015.1047794
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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