Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas

Autores
Blanco, Rancés; Carrillo-Beltrán, Diego; Muñoz, Juan P.; Osorio, Julio C.; Tapia, Julio C.; Burzio, Verónica A.; Gallegos, Iván; Calaf, Gloria M.; Chabay, Paola Andrea; Aguayo, Francisco
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein–Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low-(LSIL) and high-grade (HSIL) squamous in-traepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low-and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness.
Fil: Blanco, Rancés. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
Fil: Carrillo-Beltrán, Diego. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Muñoz, Juan P.. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Osorio, Julio C.. Universidad de Chile; Chile
Fil: Tapia, Julio C.. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
Fil: Burzio, Verónica A.. Universidad Andrés Bello; Chile
Fil: Gallegos, Iván. Universidad de Santiago de Chile. Hospital Clinico San Borja Arriaran; Chile
Fil: Calaf, Gloria M.. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Aguayo, Francisco. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
Materia
BAMHI-A RIGHTWARD FRAME 1 EXPRESSION
CERVICAL CANCER
EPSTEIN–BARR VIRUS
HUMAN PAPILLOMAVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201395
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201395
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell CarcinomasBlanco, RancésCarrillo-Beltrán, DiegoMuñoz, Juan P.Osorio, Julio C.Tapia, Julio C.Burzio, Verónica A.Gallegos, IvánCalaf, Gloria M.Chabay, Paola AndreaAguayo, FranciscoBAMHI-A RIGHTWARD FRAME 1 EXPRESSIONCERVICAL CANCEREPSTEIN–BARR VIRUSHUMAN PAPILLOMAVIRUShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein–Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low-(LSIL) and high-grade (HSIL) squamous in-traepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low-and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness.Fil: Blanco, Rancés. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; ChileFil: Carrillo-Beltrán, Diego. Universidad de Tarapaca. Instituto de Alta Investigacion.; ChileFil: Muñoz, Juan P.. Universidad de Tarapaca. Instituto de Alta Investigacion.; ChileFil: Osorio, Julio C.. Universidad de Chile; ChileFil: Tapia, Julio C.. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; ChileFil: Burzio, Verónica A.. Universidad Andrés Bello; ChileFil: Gallegos, Iván. Universidad de Santiago de Chile. Hospital Clinico San Borja Arriaran; ChileFil: Calaf, Gloria M.. Universidad de Tarapaca. Instituto de Alta Investigacion.; ChileFil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Aguayo, Francisco. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; ChileMDPI2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201395Blanco, Rancés; Carrillo-Beltrán, Diego; Muñoz, Juan P.; Osorio, Julio C.; Tapia, Julio C.; et al.; Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas; MDPI; Microorganisms; 10; 5; 5-2022; 1-172076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10050888info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:26Zoai:ri.conicet.gov.ar:11336/201395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:26.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
title Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
spellingShingle Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
Blanco, Rancés
BAMHI-A RIGHTWARD FRAME 1 EXPRESSION
CERVICAL CANCER
EPSTEIN–BARR VIRUS
HUMAN PAPILLOMAVIRUS
title_short Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
title_full Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
title_fullStr Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
title_full_unstemmed Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
title_sort Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
dc.creator.none.fl_str_mv Blanco, Rancés
Carrillo-Beltrán, Diego
Muñoz, Juan P.
Osorio, Julio C.
Tapia, Julio C.
Burzio, Verónica A.
Gallegos, Iván
Calaf, Gloria M.
Chabay, Paola Andrea
Aguayo, Francisco
author Blanco, Rancés
author_facet Blanco, Rancés
Carrillo-Beltrán, Diego
Muñoz, Juan P.
Osorio, Julio C.
Tapia, Julio C.
Burzio, Verónica A.
Gallegos, Iván
Calaf, Gloria M.
Chabay, Paola Andrea
Aguayo, Francisco
author_role author
author2 Carrillo-Beltrán, Diego
Muñoz, Juan P.
Osorio, Julio C.
Tapia, Julio C.
Burzio, Verónica A.
Gallegos, Iván
Calaf, Gloria M.
Chabay, Paola Andrea
Aguayo, Francisco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BAMHI-A RIGHTWARD FRAME 1 EXPRESSION
CERVICAL CANCER
EPSTEIN–BARR VIRUS
HUMAN PAPILLOMAVIRUS
topic BAMHI-A RIGHTWARD FRAME 1 EXPRESSION
CERVICAL CANCER
EPSTEIN–BARR VIRUS
HUMAN PAPILLOMAVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein–Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low-(LSIL) and high-grade (HSIL) squamous in-traepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low-and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness.
Fil: Blanco, Rancés. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
Fil: Carrillo-Beltrán, Diego. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Muñoz, Juan P.. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Osorio, Julio C.. Universidad de Chile; Chile
Fil: Tapia, Julio C.. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
Fil: Burzio, Verónica A.. Universidad Andrés Bello; Chile
Fil: Gallegos, Iván. Universidad de Santiago de Chile. Hospital Clinico San Borja Arriaran; Chile
Fil: Calaf, Gloria M.. Universidad de Tarapaca. Instituto de Alta Investigacion.; Chile
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; Argentina
Fil: Aguayo, Francisco. Universidad de Chile. Facultad de Medicina. Institutos de Ciencias Biomedicas.; Chile
description High-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein–Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low-(LSIL) and high-grade (HSIL) squamous in-traepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low-and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness.
publishDate 2022
dc.date.none.fl_str_mv 2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201395
Blanco, Rancés; Carrillo-Beltrán, Diego; Muñoz, Juan P.; Osorio, Julio C.; Tapia, Julio C.; et al.; Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas; MDPI; Microorganisms; 10; 5; 5-2022; 1-17
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201395
identifier_str_mv Blanco, Rancés; Carrillo-Beltrán, Diego; Muñoz, Juan P.; Osorio, Julio C.; Tapia, Julio C.; et al.; Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas; MDPI; Microorganisms; 10; 5; 5-2022; 1-17
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10050888
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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