Ethylene is involved in strawberry fruit ripening in an organ-specific manner

Autores
Merchante, Catharina; Vallarino, Jose G.; Osorio, Sonia; Aragüez, Irene; Villarreal, Natalia Marina; Ariza, Maria T.; Martinez, Gustavo Adolfo; Medina Escobar, Nieves; Civello, Pedro Marcos; Fernie, Alisdair R.; Botella, Miguel A.; Valpuesta, Victoriano
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.
Fil: Merchante, Catharina. Universidad de Málaga; España
Fil: Vallarino, Jose G.. Universidad de Málaga; España
Fil: Osorio, Sonia. Universidad de Málaga; España
Fil: Aragüez, Irene. Universidad de Málaga; España
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Ariza, Maria T.. Universidad de Málaga; España
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Medina Escobar, Nieves. Universidad de Málaga; España
Fil: Civello, Pedro Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Fernie, Alisdair R.. Institut Max Planck Fur Molekulare Physiologie; Alemania
Fil: Botella, Miguel A.. Universidad de Málaga; España
Fil: Valpuesta, Victoriano. Universidad de Málaga; España
Materia
Ethylene
Fruit
Metabolic Profiling
Non-Climacteric
Ripening
Strawberry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24279
Acceder
id CONICETDig_332408d5ad1f121b62f74f9e4069bb09
oai_identifier_str oai:ri.conicet.gov.ar:11336/24279
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ethylene is involved in strawberry fruit ripening in an organ-specific mannerMerchante, CatharinaVallarino, Jose G.Osorio, SoniaAragüez, IreneVillarreal, Natalia MarinaAriza, Maria T.Martinez, Gustavo AdolfoMedina Escobar, NievesCivello, Pedro MarcosFernie, Alisdair R.Botella, Miguel A.Valpuesta, VictorianoEthyleneFruitMetabolic ProfilingNon-ClimactericRipeningStrawberryThe fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.Fil: Merchante, Catharina. Universidad de Málaga; EspañaFil: Vallarino, Jose G.. Universidad de Málaga; EspañaFil: Osorio, Sonia. Universidad de Málaga; EspañaFil: Aragüez, Irene. Universidad de Málaga; EspañaFil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ariza, Maria T.. Universidad de Málaga; EspañaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Medina Escobar, Nieves. Universidad de Málaga; EspañaFil: Civello, Pedro Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Fernie, Alisdair R.. Institut Max Planck Fur Molekulare Physiologie; AlemaniaFil: Botella, Miguel A.. Universidad de Málaga; EspañaFil: Valpuesta, Victoriano. Universidad de Málaga; EspañaOxford University Press2013-10-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24279Merchante, Catharina; Vallarino, Jose G.; Osorio, Sonia; Aragüez, Irene; Villarreal, Natalia Marina; et al.; Ethylene is involved in strawberry fruit ripening in an organ-specific manner; Oxford University Press; Journal of Experimental Botany; 64; 14; 5-10-2013; 4421-44390022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ert257info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:55Zoai:ri.conicet.gov.ar:11336/24279instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:55.609CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ethylene is involved in strawberry fruit ripening in an organ-specific manner
title Ethylene is involved in strawberry fruit ripening in an organ-specific manner
spellingShingle Ethylene is involved in strawberry fruit ripening in an organ-specific manner
Merchante, Catharina
Ethylene
Fruit
Metabolic Profiling
Non-Climacteric
Ripening
Strawberry
title_short Ethylene is involved in strawberry fruit ripening in an organ-specific manner
title_full Ethylene is involved in strawberry fruit ripening in an organ-specific manner
title_fullStr Ethylene is involved in strawberry fruit ripening in an organ-specific manner
title_full_unstemmed Ethylene is involved in strawberry fruit ripening in an organ-specific manner
title_sort Ethylene is involved in strawberry fruit ripening in an organ-specific manner
dc.creator.none.fl_str_mv Merchante, Catharina
Vallarino, Jose G.
Osorio, Sonia
Aragüez, Irene
Villarreal, Natalia Marina
Ariza, Maria T.
Martinez, Gustavo Adolfo
Medina Escobar, Nieves
Civello, Pedro Marcos
Fernie, Alisdair R.
Botella, Miguel A.
Valpuesta, Victoriano
author Merchante, Catharina
author_facet Merchante, Catharina
Vallarino, Jose G.
Osorio, Sonia
Aragüez, Irene
Villarreal, Natalia Marina
Ariza, Maria T.
Martinez, Gustavo Adolfo
Medina Escobar, Nieves
Civello, Pedro Marcos
Fernie, Alisdair R.
Botella, Miguel A.
Valpuesta, Victoriano
author_role author
author2 Vallarino, Jose G.
Osorio, Sonia
Aragüez, Irene
Villarreal, Natalia Marina
Ariza, Maria T.
Martinez, Gustavo Adolfo
Medina Escobar, Nieves
Civello, Pedro Marcos
Fernie, Alisdair R.
Botella, Miguel A.
Valpuesta, Victoriano
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ethylene
Fruit
Metabolic Profiling
Non-Climacteric
Ripening
Strawberry
topic Ethylene
Fruit
Metabolic Profiling
Non-Climacteric
Ripening
Strawberry
dc.description.none.fl_txt_mv The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.
Fil: Merchante, Catharina. Universidad de Málaga; España
Fil: Vallarino, Jose G.. Universidad de Málaga; España
Fil: Osorio, Sonia. Universidad de Málaga; España
Fil: Aragüez, Irene. Universidad de Málaga; España
Fil: Villarreal, Natalia Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Ariza, Maria T.. Universidad de Málaga; España
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Medina Escobar, Nieves. Universidad de Málaga; España
Fil: Civello, Pedro Marcos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Fernie, Alisdair R.. Institut Max Planck Fur Molekulare Physiologie; Alemania
Fil: Botella, Miguel A.. Universidad de Málaga; España
Fil: Valpuesta, Victoriano. Universidad de Málaga; España
description The fruit of the strawberry Fragaria×ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the Fragaria vesca genome allowed for the identification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24279
Merchante, Catharina; Vallarino, Jose G.; Osorio, Sonia; Aragüez, Irene; Villarreal, Natalia Marina; et al.; Ethylene is involved in strawberry fruit ripening in an organ-specific manner; Oxford University Press; Journal of Experimental Botany; 64; 14; 5-10-2013; 4421-4439
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24279
identifier_str_mv Merchante, Catharina; Vallarino, Jose G.; Osorio, Sonia; Aragüez, Irene; Villarreal, Natalia Marina; et al.; Ethylene is involved in strawberry fruit ripening in an organ-specific manner; Oxford University Press; Journal of Experimental Botany; 64; 14; 5-10-2013; 4421-4439
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ert257
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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