Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans

Autores
Grilli, Diego Javier; Kopečný, Jan; Mrázek, Jakub; Marinšek-Logar, Romana; Paez Lama, Sebastián Antonio; Sosa Escudero, Miguel Angel; Arenas, Graciela Nora
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genes encoding glycosyl hydrolase family 11 (GH11) xylanases and xylanases have been identified from Pseudobutyrivibrio xylanivorans. In contrast, little is known about the diversity and distribution of the GH10 xylanase in strains of P. xylanivorans. Xylanase and associated activities of P. xylanivorans have been characterized in detail in the type strain, Mz5. The aim of the present study was to identify GH10 xylanase genes in strains 2 and Mz5 of P. xylanivorans. In addition, we evaluated degradation and utilization of xylan by P. xylanivorans 2 isolated from rumen of Creole goats. After a 12-h culture, P. xylanivorans 2 was able to utilize up to 53 % of the total pentose content present in birchwood xylan (BWX) and to utilize up to 62 % of a ethanol-acetic acid-soluble fraction prepared from BWX. This is the first report describing the presence of GH10 xylanase-encoding genes in P. xylanivorans. Strain 2 and Mz5 contained xylanases which were related to GH10 xylanase of Butyrivibrio sp. Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries.
Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Kopečný, Jan. Academy of Sciences of the Czech Repub; República Checa
Fil: Mrázek, Jakub. Academy of Sciences of the Czech Repub; República Checa
Fil: Marinšek-Logar, Romana. University of Ljubljana; Eslovenia
Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
Pseudobutyrivibrio
Gh10 Xilanases
Mz5
P Xilanovorans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84908
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84908
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivoransGrilli, Diego JavierKopečný, JanMrázek, JakubMarinšek-Logar, RomanaPaez Lama, Sebastián AntonioSosa Escudero, Miguel AngelArenas, Graciela NoraPseudobutyrivibrioGh10 XilanasesMz5P Xilanovoranshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Genes encoding glycosyl hydrolase family 11 (GH11) xylanases and xylanases have been identified from Pseudobutyrivibrio xylanivorans. In contrast, little is known about the diversity and distribution of the GH10 xylanase in strains of P. xylanivorans. Xylanase and associated activities of P. xylanivorans have been characterized in detail in the type strain, Mz5. The aim of the present study was to identify GH10 xylanase genes in strains 2 and Mz5 of P. xylanivorans. In addition, we evaluated degradation and utilization of xylan by P. xylanivorans 2 isolated from rumen of Creole goats. After a 12-h culture, P. xylanivorans 2 was able to utilize up to 53 % of the total pentose content present in birchwood xylan (BWX) and to utilize up to 62 % of a ethanol-acetic acid-soluble fraction prepared from BWX. This is the first report describing the presence of GH10 xylanase-encoding genes in P. xylanivorans. Strain 2 and Mz5 contained xylanases which were related to GH10 xylanase of Butyrivibrio sp. Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries.Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Kopečný, Jan. Academy of Sciences of the Czech Repub; República ChecaFil: Mrázek, Jakub. Academy of Sciences of the Czech Repub; República ChecaFil: Marinšek-Logar, Romana. University of Ljubljana; EsloveniaFil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaSpringer2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84908Grilli, Diego Javier; Kopečný, Jan; Mrázek, Jakub; Marinšek-Logar, Romana; Paez Lama, Sebastián Antonio; et al.; Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans; Springer; Folia Microbiologica; 59; 6; 10-2014; 507-5140015-5632CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12223-014-0329-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12223-014-0329-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:29Zoai:ri.conicet.gov.ar:11336/84908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:29.798CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
title Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
spellingShingle Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
Grilli, Diego Javier
Pseudobutyrivibrio
Gh10 Xilanases
Mz5
P Xilanovorans
title_short Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
title_full Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
title_fullStr Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
title_full_unstemmed Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
title_sort Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans
dc.creator.none.fl_str_mv Grilli, Diego Javier
Kopečný, Jan
Mrázek, Jakub
Marinšek-Logar, Romana
Paez Lama, Sebastián Antonio
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author Grilli, Diego Javier
author_facet Grilli, Diego Javier
Kopečný, Jan
Mrázek, Jakub
Marinšek-Logar, Romana
Paez Lama, Sebastián Antonio
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author_role author
author2 Kopečný, Jan
Mrázek, Jakub
Marinšek-Logar, Romana
Paez Lama, Sebastián Antonio
Sosa Escudero, Miguel Angel
Arenas, Graciela Nora
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pseudobutyrivibrio
Gh10 Xilanases
Mz5
P Xilanovorans
topic Pseudobutyrivibrio
Gh10 Xilanases
Mz5
P Xilanovorans
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Genes encoding glycosyl hydrolase family 11 (GH11) xylanases and xylanases have been identified from Pseudobutyrivibrio xylanivorans. In contrast, little is known about the diversity and distribution of the GH10 xylanase in strains of P. xylanivorans. Xylanase and associated activities of P. xylanivorans have been characterized in detail in the type strain, Mz5. The aim of the present study was to identify GH10 xylanase genes in strains 2 and Mz5 of P. xylanivorans. In addition, we evaluated degradation and utilization of xylan by P. xylanivorans 2 isolated from rumen of Creole goats. After a 12-h culture, P. xylanivorans 2 was able to utilize up to 53 % of the total pentose content present in birchwood xylan (BWX) and to utilize up to 62 % of a ethanol-acetic acid-soluble fraction prepared from BWX. This is the first report describing the presence of GH10 xylanase-encoding genes in P. xylanivorans. Strain 2 and Mz5 contained xylanases which were related to GH10 xylanase of Butyrivibrio sp. Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries.
Fil: Grilli, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Kopečný, Jan. Academy of Sciences of the Czech Repub; República Checa
Fil: Mrázek, Jakub. Academy of Sciences of the Czech Repub; República Checa
Fil: Marinšek-Logar, Romana. University of Ljubljana; Eslovenia
Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description Genes encoding glycosyl hydrolase family 11 (GH11) xylanases and xylanases have been identified from Pseudobutyrivibrio xylanivorans. In contrast, little is known about the diversity and distribution of the GH10 xylanase in strains of P. xylanivorans. Xylanase and associated activities of P. xylanivorans have been characterized in detail in the type strain, Mz5. The aim of the present study was to identify GH10 xylanase genes in strains 2 and Mz5 of P. xylanivorans. In addition, we evaluated degradation and utilization of xylan by P. xylanivorans 2 isolated from rumen of Creole goats. After a 12-h culture, P. xylanivorans 2 was able to utilize up to 53 % of the total pentose content present in birchwood xylan (BWX) and to utilize up to 62 % of a ethanol-acetic acid-soluble fraction prepared from BWX. This is the first report describing the presence of GH10 xylanase-encoding genes in P. xylanivorans. Strain 2 and Mz5 contained xylanases which were related to GH10 xylanase of Butyrivibrio sp. Identifying xylanase-encoding genes and activity of these enzymes are a step toward understanding possible functional role of P. xylanivorans in the rumen ecosystem and contribute to providing an improved choice of enzymes for improving fiber digestion in ruminant animals, agricultural biomass utilization for biofuel production, and other industries.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84908
Grilli, Diego Javier; Kopečný, Jan; Mrázek, Jakub; Marinšek-Logar, Romana; Paez Lama, Sebastián Antonio; et al.; Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans; Springer; Folia Microbiologica; 59; 6; 10-2014; 507-514
0015-5632
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84908
identifier_str_mv Grilli, Diego Javier; Kopečný, Jan; Mrázek, Jakub; Marinšek-Logar, Romana; Paez Lama, Sebastián Antonio; et al.; Identification of GH10 xylanases in strains 2 and Mz5 of Pseudobutyrivibrio xylanivorans; Springer; Folia Microbiologica; 59; 6; 10-2014; 507-514
0015-5632
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12223-014-0329-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12223-014-0329-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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