Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants

Autores
Capra, María Luján; Tibaldo, Maximiliano M.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Technological, biological and functional attributes of two groups of spontaneous bacteriophage-resistant mutants were investigated to verify features similar to those of their respective phage-sensitive parent probiotic strains (Lactobacillus casei/paracasei). A derivative from each group was challenged with phages in simulated probiotic product fermentation. Technological performance, compared with the original strains, was demonstrated. Minimal variations were detected in acidifying and enzymatic profiles. No β-glucuronidase activity was observed, but very high α-glucosidase and β-galactosidase activities were recorded. Low proteolytic activities and inability to ferment prebiotics were observed. Within one group, significant differences throughout simulated gastrointestinal-transit were observed. Neither of the strains deconjugated bile salts, but all showed inhibitory activity against four pathogens. Both mutants increased IgA-producing cell numbers in vivo in a manner similar to that of the parent strains. Two derivatives demonstrated improved phage-resistance phenotype compared with the parental strains, resulting in alternatives for industrial rotation schemes.
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Tibaldo, Maximiliano M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Materia
Lactobacillus Casei/Paracasei
Bacteriophages
Insensitive Mutants
Dairy Industry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8992
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8992
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutantsCapra, María LujánTibaldo, Maximiliano M.Vinderola, Celso GabrielReinheimer, Jorge AlbertoQuiberoni, Andrea del LujanLactobacillus Casei/ParacaseiBacteriophagesInsensitive MutantsDairy Industryhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Technological, biological and functional attributes of two groups of spontaneous bacteriophage-resistant mutants were investigated to verify features similar to those of their respective phage-sensitive parent probiotic strains (Lactobacillus casei/paracasei). A derivative from each group was challenged with phages in simulated probiotic product fermentation. Technological performance, compared with the original strains, was demonstrated. Minimal variations were detected in acidifying and enzymatic profiles. No β-glucuronidase activity was observed, but very high α-glucosidase and β-galactosidase activities were recorded. Low proteolytic activities and inability to ferment prebiotics were observed. Within one group, significant differences throughout simulated gastrointestinal-transit were observed. Neither of the strains deconjugated bile salts, but all showed inhibitory activity against four pathogens. Both mutants increased IgA-producing cell numbers in vivo in a manner similar to that of the parent strains. Two derivatives demonstrated improved phage-resistance phenotype compared with the parental strains, resulting in alternatives for industrial rotation schemes.Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaFil: Tibaldo, Maximiliano M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; ArgentinaElsevier2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8992Capra, María Luján; Tibaldo, Maximiliano M.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants; Elsevier; International Dairy Journal; 37; 1; -1-2014; 39-470958-6946enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.02.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614000570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:54Zoai:ri.conicet.gov.ar:11336/8992instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:54.639CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
title Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
spellingShingle Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
Capra, María Luján
Lactobacillus Casei/Paracasei
Bacteriophages
Insensitive Mutants
Dairy Industry
title_short Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
title_full Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
title_fullStr Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
title_full_unstemmed Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
title_sort Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants
dc.creator.none.fl_str_mv Capra, María Luján
Tibaldo, Maximiliano M.
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author Capra, María Luján
author_facet Capra, María Luján
Tibaldo, Maximiliano M.
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author_role author
author2 Tibaldo, Maximiliano M.
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Casei/Paracasei
Bacteriophages
Insensitive Mutants
Dairy Industry
topic Lactobacillus Casei/Paracasei
Bacteriophages
Insensitive Mutants
Dairy Industry
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Technological, biological and functional attributes of two groups of spontaneous bacteriophage-resistant mutants were investigated to verify features similar to those of their respective phage-sensitive parent probiotic strains (Lactobacillus casei/paracasei). A derivative from each group was challenged with phages in simulated probiotic product fermentation. Technological performance, compared with the original strains, was demonstrated. Minimal variations were detected in acidifying and enzymatic profiles. No β-glucuronidase activity was observed, but very high α-glucosidase and β-galactosidase activities were recorded. Low proteolytic activities and inability to ferment prebiotics were observed. Within one group, significant differences throughout simulated gastrointestinal-transit were observed. Neither of the strains deconjugated bile salts, but all showed inhibitory activity against four pathogens. Both mutants increased IgA-producing cell numbers in vivo in a manner similar to that of the parent strains. Two derivatives demonstrated improved phage-resistance phenotype compared with the parental strains, resulting in alternatives for industrial rotation schemes.
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Tibaldo, Maximiliano M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactologia Industrial; Argentina
description Technological, biological and functional attributes of two groups of spontaneous bacteriophage-resistant mutants were investigated to verify features similar to those of their respective phage-sensitive parent probiotic strains (Lactobacillus casei/paracasei). A derivative from each group was challenged with phages in simulated probiotic product fermentation. Technological performance, compared with the original strains, was demonstrated. Minimal variations were detected in acidifying and enzymatic profiles. No β-glucuronidase activity was observed, but very high α-glucosidase and β-galactosidase activities were recorded. Low proteolytic activities and inability to ferment prebiotics were observed. Within one group, significant differences throughout simulated gastrointestinal-transit were observed. Neither of the strains deconjugated bile salts, but all showed inhibitory activity against four pathogens. Both mutants increased IgA-producing cell numbers in vivo in a manner similar to that of the parent strains. Two derivatives demonstrated improved phage-resistance phenotype compared with the parental strains, resulting in alternatives for industrial rotation schemes.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8992
Capra, María Luján; Tibaldo, Maximiliano M.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants; Elsevier; International Dairy Journal; 37; 1; -1-2014; 39-47
0958-6946
url http://hdl.handle.net/11336/8992
identifier_str_mv Capra, María Luján; Tibaldo, Maximiliano M.; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Technological and probiotic characterisation of Lactobacillus casei / paracasei strains and their phage resistant mutants; Elsevier; International Dairy Journal; 37; 1; -1-2014; 39-47
0958-6946
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.02.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614000570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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