Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei

Autores
Capra, María Luján; Quiberoni, Andrea del Lujan; Ackermann, H. W.; Moineau, S.; Reinheimer, Jorge Alberto
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new virulent bacteriophage (MLC-A) was recently isolated in Argentina from a probiotic dairy product containing a strain of Lactobacillus paracasei. Observation of the lysate with an electron microscope revealed bacteriophage particles with an icosahedral capsid of 57 ± 2 nm; with a collar and a noncontractile tail of 156 ± 3 nm terminating with a baseplate to which a tail fiber was attached. Therefore, phage MLC-A belongs to the Siphoviridae family. This phage was able to survive the pasteurization process and was resistant to alcohols and sodium hypochlorite (400 mg/kg). Only peracetic acid could inactivate high-titer suspensions of phages in a short time. The maximum rates of phage adsorption to its host cells were obtained at 30°C with a pH between 5 and 7, and in the presence of calcium or magnesium ions. The host range of phage MLC-A encompassed L. paracasei and Lactobacillus casei strains, but it was not able to  nfect Lactobacillus rhamnosus or Lactobacillus gasseri strains. One-step growth kinetics of its lyticdevelopment revealed latent and burst periods of 30 and 135 min, respectively, with a burst size of about 69 ± 4 plaque-forming units per infected cell. Phage MLC-A had a distinctive restriction profile when compared with the 2 well-studied Lactobacillus phages, PL-1 and J-1. The genome size of the MLC-A phage was estimated to be approximately 37 kb. This study presents the description of the first phage specific for L. paracasei isolated in Argentina. The isolation of phage MLC-A indicates that, beside lactic acid bacteria starters, MLC-A indicates that, beside lactic acid bacteria starters, probiotic cultures can also be sensitive to virulent phages in industrial processes.
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Ackermann, H. W.. Laval University. Centre Hospitalier de L'universite Laval; Canadá
Fil: Moineau, S.. Laval University. Centre Hospitalier de L'universite Laval; Canadá
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
PHAGES
LACTOBACILLUS PARACASEI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113449
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113449
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracaseiCapra, María LujánQuiberoni, Andrea del LujanAckermann, H. W.Moineau, S.Reinheimer, Jorge AlbertoPHAGESLACTOBACILLUS PARACASEIhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2A new virulent bacteriophage (MLC-A) was recently isolated in Argentina from a probiotic dairy product containing a strain of Lactobacillus paracasei. Observation of the lysate with an electron microscope revealed bacteriophage particles with an icosahedral capsid of 57 ± 2 nm; with a collar and a noncontractile tail of 156 ± 3 nm terminating with a baseplate to which a tail fiber was attached. Therefore, phage MLC-A belongs to the Siphoviridae family. This phage was able to survive the pasteurization process and was resistant to alcohols and sodium hypochlorite (400 mg/kg). Only peracetic acid could inactivate high-titer suspensions of phages in a short time. The maximum rates of phage adsorption to its host cells were obtained at 30°C with a pH between 5 and 7, and in the presence of calcium or magnesium ions. The host range of phage MLC-A encompassed L. paracasei and Lactobacillus casei strains, but it was not able to  nfect Lactobacillus rhamnosus or Lactobacillus gasseri strains. One-step growth kinetics of its lyticdevelopment revealed latent and burst periods of 30 and 135 min, respectively, with a burst size of about 69 ± 4 plaque-forming units per infected cell. Phage MLC-A had a distinctive restriction profile when compared with the 2 well-studied Lactobacillus phages, PL-1 and J-1. The genome size of the MLC-A phage was estimated to be approximately 37 kb. This study presents the description of the first phage specific for L. paracasei isolated in Argentina. The isolation of phage MLC-A indicates that, beside lactic acid bacteria starters, MLC-A indicates that, beside lactic acid bacteria starters, probiotic cultures can also be sensitive to virulent phages in industrial processes.Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Ackermann, H. W.. Laval University. Centre Hospitalier de L'universite Laval; CanadáFil: Moineau, S.. Laval University. Centre Hospitalier de L'universite Laval; CanadáFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaAmerican Dairy Science Association2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113449Capra, María Luján; Quiberoni, Andrea del Lujan; Ackermann, H. W.; Moineau, S.; Reinheimer, Jorge Alberto; Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei; American Dairy Science Association; Journal of Dairy Science; 89; 7; 12-2006; 2414-24230022-0302CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3168/jds.S0022-0302(06)72314-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:02Zoai:ri.conicet.gov.ar:11336/113449instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:02.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
title Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
spellingShingle Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
Capra, María Luján
PHAGES
LACTOBACILLUS PARACASEI
title_short Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
title_full Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
title_fullStr Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
title_full_unstemmed Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
title_sort Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei
dc.creator.none.fl_str_mv Capra, María Luján
Quiberoni, Andrea del Lujan
Ackermann, H. W.
Moineau, S.
Reinheimer, Jorge Alberto
author Capra, María Luján
author_facet Capra, María Luján
Quiberoni, Andrea del Lujan
Ackermann, H. W.
Moineau, S.
Reinheimer, Jorge Alberto
author_role author
author2 Quiberoni, Andrea del Lujan
Ackermann, H. W.
Moineau, S.
Reinheimer, Jorge Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHAGES
LACTOBACILLUS PARACASEI
topic PHAGES
LACTOBACILLUS PARACASEI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A new virulent bacteriophage (MLC-A) was recently isolated in Argentina from a probiotic dairy product containing a strain of Lactobacillus paracasei. Observation of the lysate with an electron microscope revealed bacteriophage particles with an icosahedral capsid of 57 ± 2 nm; with a collar and a noncontractile tail of 156 ± 3 nm terminating with a baseplate to which a tail fiber was attached. Therefore, phage MLC-A belongs to the Siphoviridae family. This phage was able to survive the pasteurization process and was resistant to alcohols and sodium hypochlorite (400 mg/kg). Only peracetic acid could inactivate high-titer suspensions of phages in a short time. The maximum rates of phage adsorption to its host cells were obtained at 30°C with a pH between 5 and 7, and in the presence of calcium or magnesium ions. The host range of phage MLC-A encompassed L. paracasei and Lactobacillus casei strains, but it was not able to  nfect Lactobacillus rhamnosus or Lactobacillus gasseri strains. One-step growth kinetics of its lyticdevelopment revealed latent and burst periods of 30 and 135 min, respectively, with a burst size of about 69 ± 4 plaque-forming units per infected cell. Phage MLC-A had a distinctive restriction profile when compared with the 2 well-studied Lactobacillus phages, PL-1 and J-1. The genome size of the MLC-A phage was estimated to be approximately 37 kb. This study presents the description of the first phage specific for L. paracasei isolated in Argentina. The isolation of phage MLC-A indicates that, beside lactic acid bacteria starters, MLC-A indicates that, beside lactic acid bacteria starters, probiotic cultures can also be sensitive to virulent phages in industrial processes.
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Ackermann, H. W.. Laval University. Centre Hospitalier de L'universite Laval; Canadá
Fil: Moineau, S.. Laval University. Centre Hospitalier de L'universite Laval; Canadá
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description A new virulent bacteriophage (MLC-A) was recently isolated in Argentina from a probiotic dairy product containing a strain of Lactobacillus paracasei. Observation of the lysate with an electron microscope revealed bacteriophage particles with an icosahedral capsid of 57 ± 2 nm; with a collar and a noncontractile tail of 156 ± 3 nm terminating with a baseplate to which a tail fiber was attached. Therefore, phage MLC-A belongs to the Siphoviridae family. This phage was able to survive the pasteurization process and was resistant to alcohols and sodium hypochlorite (400 mg/kg). Only peracetic acid could inactivate high-titer suspensions of phages in a short time. The maximum rates of phage adsorption to its host cells were obtained at 30°C with a pH between 5 and 7, and in the presence of calcium or magnesium ions. The host range of phage MLC-A encompassed L. paracasei and Lactobacillus casei strains, but it was not able to  nfect Lactobacillus rhamnosus or Lactobacillus gasseri strains. One-step growth kinetics of its lyticdevelopment revealed latent and burst periods of 30 and 135 min, respectively, with a burst size of about 69 ± 4 plaque-forming units per infected cell. Phage MLC-A had a distinctive restriction profile when compared with the 2 well-studied Lactobacillus phages, PL-1 and J-1. The genome size of the MLC-A phage was estimated to be approximately 37 kb. This study presents the description of the first phage specific for L. paracasei isolated in Argentina. The isolation of phage MLC-A indicates that, beside lactic acid bacteria starters, MLC-A indicates that, beside lactic acid bacteria starters, probiotic cultures can also be sensitive to virulent phages in industrial processes.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113449
Capra, María Luján; Quiberoni, Andrea del Lujan; Ackermann, H. W.; Moineau, S.; Reinheimer, Jorge Alberto; Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei; American Dairy Science Association; Journal of Dairy Science; 89; 7; 12-2006; 2414-2423
0022-0302
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113449
identifier_str_mv Capra, María Luján; Quiberoni, Andrea del Lujan; Ackermann, H. W.; Moineau, S.; Reinheimer, Jorge Alberto; Characterization of a New Virulent Phage (MLC-A) of Lactobacillus paracasei; American Dairy Science Association; Journal of Dairy Science; 89; 7; 12-2006; 2414-2423
0022-0302
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.S0022-0302(06)72314-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Dairy Science Association
publisher.none.fl_str_mv American Dairy Science Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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