Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum

Autores
Leguina, Ana Carolina del Valle; Lacosegliaz, Mariano José; Fernández, Pablo Marcelo; Castellanos, Lucia Ines; Nieto Peñalver, Carlos Gabriel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Copper has largely been used for the control of phytopathogen fungi in agriculture, even though to its non-degradability, it tends to accumulate in soils reaching prejudicial levels for soil microorganisms, including rhizomicroorganisms. The rhizosphere is characterized by intense and complex interactions that take place in it. Many of these intra- and interspecies interactions occur through quorum sensing (QS) systems. QS is a cell-to-cell signaling mechanism that control the microbial physiology in a population density manner. Several soil bacteria use QS circuits to regulate important phenotypes. In this work we studied the influence of copper on QS regulation in the plant growth-promoting rhizobacterium (PGPR) Pseudomonas capeferrum WCS358. Firstly, the QS system of the bacterium was inactivated using a quorum quenching strategy. Secondly, intracellular proteins of Ps. capeferrum WCS358 QS+ and QS-, cultured in the presence or absence of copper, were analyzed using liquid chromatography coupled to mass spectrometry. Furthermore, the effects of copper and QS on other activities such us motility, biofilm production and oxidative stress response were also evaluated in Ps. capeferrum WCS358. The QS activity and the presence of metal modified the relative abundance of proteins involved in amino acid and carbohydrate metabolism, oxidative stress defense and nutrient absorption. Besides, results indicated that QS system is implicated in the regulation of motility, biofilm production and oxidative stress response in Ps. capeferrum WCS358 and that copper had a negative effect on these activities. The results presented in this work indicate that QS regulates important traits in Ps. capeferrum WCS358 and that contamination with copper could be detrimental for the QS-dependent phenotypes in this rhizobacterium. Since the modifications observed are related to activities that are significant for the survival and fitness of bacteria, they suggest that QS may confer a competitive advantage to Ps. capeferrum WCS358 and that copper could alter the competence of this PGPR in its natural niche.
Fil: Leguina, Ana Carolina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fernández, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología
Sociedad Argentina de Microbiología General
Materia
QUORUM SENSING
COPPER
INTERACTIONS
RHIZOSPHERE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181505
Acceder
id CONICETDig_308a16c19862e75c5a12765ac0d57ff1
oai_identifier_str oai:ri.conicet.gov.ar:11336/181505
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrumLeguina, Ana Carolina del ValleLacosegliaz, Mariano JoséFernández, Pablo MarceloCastellanos, Lucia InesNieto Peñalver, Carlos GabrielQUORUM SENSINGCOPPERINTERACTIONSRHIZOSPHEREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Copper has largely been used for the control of phytopathogen fungi in agriculture, even though to its non-degradability, it tends to accumulate in soils reaching prejudicial levels for soil microorganisms, including rhizomicroorganisms. The rhizosphere is characterized by intense and complex interactions that take place in it. Many of these intra- and interspecies interactions occur through quorum sensing (QS) systems. QS is a cell-to-cell signaling mechanism that control the microbial physiology in a population density manner. Several soil bacteria use QS circuits to regulate important phenotypes. In this work we studied the influence of copper on QS regulation in the plant growth-promoting rhizobacterium (PGPR) Pseudomonas capeferrum WCS358. Firstly, the QS system of the bacterium was inactivated using a quorum quenching strategy. Secondly, intracellular proteins of Ps. capeferrum WCS358 QS+ and QS-, cultured in the presence or absence of copper, were analyzed using liquid chromatography coupled to mass spectrometry. Furthermore, the effects of copper and QS on other activities such us motility, biofilm production and oxidative stress response were also evaluated in Ps. capeferrum WCS358. The QS activity and the presence of metal modified the relative abundance of proteins involved in amino acid and carbohydrate metabolism, oxidative stress defense and nutrient absorption. Besides, results indicated that QS system is implicated in the regulation of motility, biofilm production and oxidative stress response in Ps. capeferrum WCS358 and that copper had a negative effect on these activities. The results presented in this work indicate that QS regulates important traits in Ps. capeferrum WCS358 and that contamination with copper could be detrimental for the QS-dependent phenotypes in this rhizobacterium. Since the modifications observed are related to activities that are significant for the survival and fitness of bacteria, they suggest that QS may confer a competitive advantage to Ps. capeferrum WCS358 and that copper could alter the competence of this PGPR in its natural niche.Fil: Leguina, Ana Carolina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fernández, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General MicrobiologyCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación Bioquímica y BiologíaSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181505Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:54Zoai:ri.conicet.gov.ar:11336/181505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:54.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
title Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
spellingShingle Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
Leguina, Ana Carolina del Valle
QUORUM SENSING
COPPER
INTERACTIONS
RHIZOSPHERE
title_short Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
title_full Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
title_fullStr Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
title_full_unstemmed Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
title_sort Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum
dc.creator.none.fl_str_mv Leguina, Ana Carolina del Valle
Lacosegliaz, Mariano José
Fernández, Pablo Marcelo
Castellanos, Lucia Ines
Nieto Peñalver, Carlos Gabriel
author Leguina, Ana Carolina del Valle
author_facet Leguina, Ana Carolina del Valle
Lacosegliaz, Mariano José
Fernández, Pablo Marcelo
Castellanos, Lucia Ines
Nieto Peñalver, Carlos Gabriel
author_role author
author2 Lacosegliaz, Mariano José
Fernández, Pablo Marcelo
Castellanos, Lucia Ines
Nieto Peñalver, Carlos Gabriel
author2_role author
author
author
author
dc.subject.none.fl_str_mv QUORUM SENSING
COPPER
INTERACTIONS
RHIZOSPHERE
topic QUORUM SENSING
COPPER
INTERACTIONS
RHIZOSPHERE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Copper has largely been used for the control of phytopathogen fungi in agriculture, even though to its non-degradability, it tends to accumulate in soils reaching prejudicial levels for soil microorganisms, including rhizomicroorganisms. The rhizosphere is characterized by intense and complex interactions that take place in it. Many of these intra- and interspecies interactions occur through quorum sensing (QS) systems. QS is a cell-to-cell signaling mechanism that control the microbial physiology in a population density manner. Several soil bacteria use QS circuits to regulate important phenotypes. In this work we studied the influence of copper on QS regulation in the plant growth-promoting rhizobacterium (PGPR) Pseudomonas capeferrum WCS358. Firstly, the QS system of the bacterium was inactivated using a quorum quenching strategy. Secondly, intracellular proteins of Ps. capeferrum WCS358 QS+ and QS-, cultured in the presence or absence of copper, were analyzed using liquid chromatography coupled to mass spectrometry. Furthermore, the effects of copper and QS on other activities such us motility, biofilm production and oxidative stress response were also evaluated in Ps. capeferrum WCS358. The QS activity and the presence of metal modified the relative abundance of proteins involved in amino acid and carbohydrate metabolism, oxidative stress defense and nutrient absorption. Besides, results indicated that QS system is implicated in the regulation of motility, biofilm production and oxidative stress response in Ps. capeferrum WCS358 and that copper had a negative effect on these activities. The results presented in this work indicate that QS regulates important traits in Ps. capeferrum WCS358 and that contamination with copper could be detrimental for the QS-dependent phenotypes in this rhizobacterium. Since the modifications observed are related to activities that are significant for the survival and fitness of bacteria, they suggest that QS may confer a competitive advantage to Ps. capeferrum WCS358 and that copper could alter the competence of this PGPR in its natural niche.
Fil: Leguina, Ana Carolina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fernández, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Nieto Peñalver, Carlos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología
Sociedad Argentina de Microbiología General
description Copper has largely been used for the control of phytopathogen fungi in agriculture, even though to its non-degradability, it tends to accumulate in soils reaching prejudicial levels for soil microorganisms, including rhizomicroorganisms. The rhizosphere is characterized by intense and complex interactions that take place in it. Many of these intra- and interspecies interactions occur through quorum sensing (QS) systems. QS is a cell-to-cell signaling mechanism that control the microbial physiology in a population density manner. Several soil bacteria use QS circuits to regulate important phenotypes. In this work we studied the influence of copper on QS regulation in the plant growth-promoting rhizobacterium (PGPR) Pseudomonas capeferrum WCS358. Firstly, the QS system of the bacterium was inactivated using a quorum quenching strategy. Secondly, intracellular proteins of Ps. capeferrum WCS358 QS+ and QS-, cultured in the presence or absence of copper, were analyzed using liquid chromatography coupled to mass spectrometry. Furthermore, the effects of copper and QS on other activities such us motility, biofilm production and oxidative stress response were also evaluated in Ps. capeferrum WCS358. The QS activity and the presence of metal modified the relative abundance of proteins involved in amino acid and carbohydrate metabolism, oxidative stress defense and nutrient absorption. Besides, results indicated that QS system is implicated in the regulation of motility, biofilm production and oxidative stress response in Ps. capeferrum WCS358 and that copper had a negative effect on these activities. The results presented in this work indicate that QS regulates important traits in Ps. capeferrum WCS358 and that contamination with copper could be detrimental for the QS-dependent phenotypes in this rhizobacterium. Since the modifications observed are related to activities that are significant for the survival and fitness of bacteria, they suggest that QS may confer a competitive advantage to Ps. capeferrum WCS358 and that copper could alter the competence of this PGPR in its natural niche.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181505
Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181505
identifier_str_mv Proteomic and physiological characterization of copper effect on quorum sensing regulation in Pseudomonas capeferrum; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 1-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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