Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin...

Autores: Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Lazarte Otero, Valeria Sabrina; Escudero, María Esther
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: This study was aimed to determine the prevalence of Salmonella spp. in foods of animal origin sold at retail stores over the period 2005–2011 in San Luis, Argentina. Characterization of isolates was performed by biochemical and serological tests, antimicrobial susceptibility assays, detection of invA invasion gene by PCR and comparison of genomic profiles by XbaI DNA restriction and PFGE. Twenty seven Salmonella strains were detected in 27 (6.32%) of 427 samples of foods analysed. Sixteen S. Enteritidis and one S. Montevideo strains from chicken meat (17 positive samples/115 total samples), six S. Anatum strains from pork sausages (6/90), two S. Typhimurium strains from liquid egg (2/60) and two S. Montevideo strains from chicken giblets (2/62) were isolated. No Salmonella strains were recovered from chicken carcasses (0/100). Salmonella strains were susceptible to antimicrobials commonly used for clinical treatment. All isolates carried the invA gene. DNA restriction and PFGE analysis revealed similar genomic profiles within each Salmonella serovar regardless of the food type, sampling year, or retail store where samples were purchased, suggesting the possibility of circulation and transmission of clones of limited diversity in our region.
Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lazarte Otero, Valeria Sabrina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia: Salmonella
Foods
Prevalence
Antimicrobial Susceptibility
Inva Gene
Pfge
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/26776

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spelling	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, ArgentinaFavier, Gabriela IsabelLucero Estrada, Cecilia Stella MarysLazarte Otero, Valeria SabrinaEscudero, María EstherSalmonellaFoodsPrevalenceAntimicrobial SusceptibilityInva GenePfgehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study was aimed to determine the prevalence of Salmonella spp. in foods of animal origin sold at retail stores over the period 2005–2011 in San Luis, Argentina. Characterization of isolates was performed by biochemical and serological tests, antimicrobial susceptibility assays, detection of invA invasion gene by PCR and comparison of genomic profiles by XbaI DNA restriction and PFGE. Twenty seven Salmonella strains were detected in 27 (6.32%) of 427 samples of foods analysed. Sixteen S. Enteritidis and one S. Montevideo strains from chicken meat (17 positive samples/115 total samples), six S. Anatum strains from pork sausages (6/90), two S. Typhimurium strains from liquid egg (2/60) and two S. Montevideo strains from chicken giblets (2/62) were isolated. No Salmonella strains were recovered from chicken carcasses (0/100). Salmonella strains were susceptible to antimicrobials commonly used for clinical treatment. All isolates carried the invA gene. DNA restriction and PFGE analysis revealed similar genomic profiles within each Salmonella serovar regardless of the food type, sampling year, or retail store where samples were purchased, suggesting the possibility of circulation and transmission of clones of limited diversity in our region.Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lazarte Otero, Valeria Sabrina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26776Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Lazarte Otero, Valeria Sabrina; Escudero, María Esther; Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina; Elsevier; Food Control; 29; 1; 1-2013; 49-540956-7135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956713512002903info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2012.05.056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:14:26Zoai:ri.conicet.gov.ar:11336/26776instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:14:27.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
title	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
spellingShingle	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina Favier, Gabriela Isabel Salmonella Foods Prevalence Antimicrobial Susceptibility Inva Gene Pfge
title_short	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
title_full	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
title_fullStr	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
title_full_unstemmed	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
title_sort	Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina
dc.creator.none.fl_str_mv	Favier, Gabriela Isabel Lucero Estrada, Cecilia Stella Marys Lazarte Otero, Valeria Sabrina Escudero, María Esther
author	Favier, Gabriela Isabel
author_facet	Favier, Gabriela Isabel Lucero Estrada, Cecilia Stella Marys Lazarte Otero, Valeria Sabrina Escudero, María Esther
author_role	author
author2	Lucero Estrada, Cecilia Stella Marys Lazarte Otero, Valeria Sabrina Escudero, María Esther
author2_role	author author author
dc.subject.none.fl_str_mv	Salmonella Foods Prevalence Antimicrobial Susceptibility Inva Gene Pfge
topic	Salmonella Foods Prevalence Antimicrobial Susceptibility Inva Gene Pfge
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	This study was aimed to determine the prevalence of Salmonella spp. in foods of animal origin sold at retail stores over the period 2005–2011 in San Luis, Argentina. Characterization of isolates was performed by biochemical and serological tests, antimicrobial susceptibility assays, detection of invA invasion gene by PCR and comparison of genomic profiles by XbaI DNA restriction and PFGE. Twenty seven Salmonella strains were detected in 27 (6.32%) of 427 samples of foods analysed. Sixteen S. Enteritidis and one S. Montevideo strains from chicken meat (17 positive samples/115 total samples), six S. Anatum strains from pork sausages (6/90), two S. Typhimurium strains from liquid egg (2/60) and two S. Montevideo strains from chicken giblets (2/62) were isolated. No Salmonella strains were recovered from chicken carcasses (0/100). Salmonella strains were susceptible to antimicrobials commonly used for clinical treatment. All isolates carried the invA gene. DNA restriction and PFGE analysis revealed similar genomic profiles within each Salmonella serovar regardless of the food type, sampling year, or retail store where samples were purchased, suggesting the possibility of circulation and transmission of clones of limited diversity in our region. Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lazarte Otero, Valeria Sabrina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description	This study was aimed to determine the prevalence of Salmonella spp. in foods of animal origin sold at retail stores over the period 2005–2011 in San Luis, Argentina. Characterization of isolates was performed by biochemical and serological tests, antimicrobial susceptibility assays, detection of invA invasion gene by PCR and comparison of genomic profiles by XbaI DNA restriction and PFGE. Twenty seven Salmonella strains were detected in 27 (6.32%) of 427 samples of foods analysed. Sixteen S. Enteritidis and one S. Montevideo strains from chicken meat (17 positive samples/115 total samples), six S. Anatum strains from pork sausages (6/90), two S. Typhimurium strains from liquid egg (2/60) and two S. Montevideo strains from chicken giblets (2/62) were isolated. No Salmonella strains were recovered from chicken carcasses (0/100). Salmonella strains were susceptible to antimicrobials commonly used for clinical treatment. All isolates carried the invA gene. DNA restriction and PFGE analysis revealed similar genomic profiles within each Salmonella serovar regardless of the food type, sampling year, or retail store where samples were purchased, suggesting the possibility of circulation and transmission of clones of limited diversity in our region.
publishDate	2013
dc.date.none.fl_str_mv	2013-01
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/26776 Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Lazarte Otero, Valeria Sabrina; Escudero, María Esther; Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina; Elsevier; Food Control; 29; 1; 1-2013; 49-54 0956-7135 CONICET Digital CONICET
url	http://hdl.handle.net/11336/26776
identifier_str_mv	Favier, Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Lazarte Otero, Valeria Sabrina; Escudero, María Esther; Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina; Elsevier; Food Control; 29; 1; 1-2013; 49-54 0956-7135 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956713512002903 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodcont.2012.05.056
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	12.982451

Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin...

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