Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis

Autores
Gornatti Churria, Carlos Daniel; Vigo, Germán Blas; Origlia, Javier Aníbal; Campos, Josefina; Caffer, María; Píscopo, Miguel Víctor; Herrero Loyola, Miguel Ángel; Petruccelli, Miguel Ángel; Pichel, Mariana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.
Empleando estudios anatomopatológicos y microbiológicos se examinó a un grupo de chinchillas (Chinchilla lanigera) adultas que murieron súbitamente en 2012 en una granja de la ciudad de La Plata (Buenos Aires, Argentina). Se aisló Salmonella enterica serovar Typhimurium (S. Typhimurium) del hígado, el bazo, el corazón, los pulmones, los riñones y los intestinos de los cinco animales evaluados. Los cinco aislamientos estudiados (uno por animal) fueron sensibles a ampicilina, cefalotina, cefotaxima, ácido nalidíxico, gentamicina, estreptomicina, cloranfenicol, fosfomicina, nitrofurantoína y trimetoprima-sulfametoxazol, y resistentes a tetraciclina. El análisis de dichos aislamientos por electroforesis en gel de campo pulsado [pulsed-fi eld gel electrophoresis (PFGE)] con XbaI mostró un perÀ l electroforético idéntico con 15 bandas, idéntico a su vez al patrón ARJPXX01.0220 del banco nacional argentino de datos de PulseNet, que cuenta con patrones de PFGE de Salmonella. El presente trabajo describe por primera vez el diagnóstico postmortem de un brote de salmonelosis en chinchillas usando un método molecular, como la electroforesis en gel en campo pulsado.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85125

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85125
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresisDiagnóstico de un brote de Salmonella Typhimurium en chinchillas (Chinchilla lanigera) mediante la electroforesis en gel de campo pulsadoGornatti Churria, Carlos DanielVigo, Germán BlasOriglia, Javier AníbalCampos, JosefinaCaffer, MaríaPíscopo, Miguel VíctorHerrero Loyola, Miguel ÁngelPetruccelli, Miguel ÁngelPichel, MarianaCiencias VeterinariasChinchilla lanigeraFarmed chinchillasPFGESalmonella TyphimuriumZoonosisAdult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.Empleando estudios anatomopatológicos y microbiológicos se examinó a un grupo de chinchillas (Chinchilla lanigera) adultas que murieron súbitamente en 2012 en una granja de la ciudad de La Plata (Buenos Aires, Argentina). Se aisló Salmonella enterica serovar Typhimurium (S. Typhimurium) del hígado, el bazo, el corazón, los pulmones, los riñones y los intestinos de los cinco animales evaluados. Los cinco aislamientos estudiados (uno por animal) fueron sensibles a ampicilina, cefalotina, cefotaxima, ácido nalidíxico, gentamicina, estreptomicina, cloranfenicol, fosfomicina, nitrofurantoína y trimetoprima-sulfametoxazol, y resistentes a tetraciclina. El análisis de dichos aislamientos por electroforesis en gel de campo pulsado [pulsed-fi eld gel electrophoresis (PFGE)] con XbaI mostró un perÀ l electroforético idéntico con 15 bandas, idéntico a su vez al patrón ARJPXX01.0220 del banco nacional argentino de datos de PulseNet, que cuenta con patrones de PFGE de Salmonella. El presente trabajo describe por primera vez el diagnóstico postmortem de un brote de salmonelosis en chinchillas usando un método molecular, como la electroforesis en gel en campo pulsado.Facultad de Ciencias Veterinarias2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf205-209http://sedici.unlp.edu.ar/handle/10915/85125enginfo:eu-repo/semantics/altIdentifier/issn/0325-7541info:eu-repo/semantics/altIdentifier/doi/10.1016/S0325-7541(14)70074-4info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:20Zoai:sedici.unlp.edu.ar:10915/85125Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:21.278SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
Diagnóstico de un brote de Salmonella Typhimurium en chinchillas (Chinchilla lanigera) mediante la electroforesis en gel de campo pulsado
title Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
spellingShingle Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
Gornatti Churria, Carlos Daniel
Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
title_short Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_full Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_fullStr Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_full_unstemmed Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
title_sort Diagnosis of an outbreak of Salmonella Typhimurium in chinchillas (Chinchilla lanigera) by pulsed-field gel electrophoresis
dc.creator.none.fl_str_mv Gornatti Churria, Carlos Daniel
Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
author Gornatti Churria, Carlos Daniel
author_facet Gornatti Churria, Carlos Daniel
Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
author_role author
author2 Vigo, Germán Blas
Origlia, Javier Aníbal
Campos, Josefina
Caffer, María
Píscopo, Miguel Víctor
Herrero Loyola, Miguel Ángel
Petruccelli, Miguel Ángel
Pichel, Mariana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
topic Ciencias Veterinarias
Chinchilla lanigera
Farmed chinchillas
PFGE
Salmonella Typhimurium
Zoonosis
dc.description.none.fl_txt_mv Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.
Empleando estudios anatomopatológicos y microbiológicos se examinó a un grupo de chinchillas (Chinchilla lanigera) adultas que murieron súbitamente en 2012 en una granja de la ciudad de La Plata (Buenos Aires, Argentina). Se aisló Salmonella enterica serovar Typhimurium (S. Typhimurium) del hígado, el bazo, el corazón, los pulmones, los riñones y los intestinos de los cinco animales evaluados. Los cinco aislamientos estudiados (uno por animal) fueron sensibles a ampicilina, cefalotina, cefotaxima, ácido nalidíxico, gentamicina, estreptomicina, cloranfenicol, fosfomicina, nitrofurantoína y trimetoprima-sulfametoxazol, y resistentes a tetraciclina. El análisis de dichos aislamientos por electroforesis en gel de campo pulsado [pulsed-fi eld gel electrophoresis (PFGE)] con XbaI mostró un perÀ l electroforético idéntico con 15 bandas, idéntico a su vez al patrón ARJPXX01.0220 del banco nacional argentino de datos de PulseNet, que cuenta con patrones de PFGE de Salmonella. El presente trabajo describe por primera vez el diagnóstico postmortem de un brote de salmonelosis en chinchillas usando un método molecular, como la electroforesis en gel en campo pulsado.
Facultad de Ciencias Veterinarias
description Adult chinchillas (Chinchilla lanigera) that had suddenly died in a commercial farm located in La Plata City, Buenos Aires Province, Argentina, in July 2012 were macroscopically, histopathologically, and microbiologically examined. Salmonella enterica serovar Typhimurium (S. Typhimurium) was isolated from the liver, spleen, heart, lungs, kidneys and intestines from each of the five animals evaluated. The five strains were susceptible to ampicillin, cephalotin, cefotaxime, nalidixic acid, gentamicin, streptomycin, chloramphenicol, fosfomycin, nitrofurantoin and trimethoprimsulfamethoxazole, and resistant to tetracycline. Each of the five S. Typhimurium isolates was analyzed by XbaI-pulsed-field gel electrophoresis (PFGE), showing an identical electrophoretic profile with 15 defined bands, which was found to be identical to pattern ARJPXX01.0220 of the PulseNet Argentine National database of Salmonella PFGE patterns. This is the first work describing the postmortem diagnosis of an outbreak of salmonellosis in chinchillas by using molecular methods such as PFGE.
publishDate 2014
dc.date.none.fl_str_mv 2014
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http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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205-209
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