Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry
- Autores
- Isaguirre, Andrea Celeste; Santos, Rocío; Quiroga Aromataris, Santiago Gabriel; Amieva, María Itatí; Delfini, Claudio Daniel; Villegas, Liliana Beatriz
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Food losses due to the presence of microorganisms, such as fungi, are a major concern worldwide, especially with a constantly growing worldpopulation. The development of these organisms leads to sensory defects in food ranging from visual deterioration to noticeable changes in smell, tasteor texture, but they can also have negative impacts on health due to the production of mycotoxins by some species. The objective of this work was toevaluate antifungal activity on fungi of the genus Aspergillus in the presence of Cu, Calcium Propionate and Potassium Sorbate, which are used asantifungals in agriculture and the food industry. The cytotoxicity also was evaluated. Fungi isolated and identified in previous work from spoiled breadwere used: Aspergillus niger and A. tritici. The proposed methodology included different concentrations of sodium propionate and sorbate (0.15?2 %w/v) in the presence of the microorganism under study in liquid Luria-Bertani (LB) culture medium. On the other hand, the antifungal activity ofdifferent additives was determined by measuring mycelial growth on extract-glucose agar (EG), with and without the additives. For the copper effect,a similar methodology with 400 ppm. For the cytotoxicity assay, Artemia salina eggs were incubated in saltwater at 27°C for 48 hours until the naupliihatched. The nauplii were exposed to the additive solution. The results showed inhibition of the growth of both fungi species with the different additivessolutions used. Propionate inhibited the growth of A. niger at a concentration of 2%, while for A. tritici was more resistant but a decrease in growthwas observed, compared to the positive control. With potassium sorbate it was observed that at concentrations of 0.5% and 1% no growth of A. nigerand A. tritici was observed, respectively. Finally, the experiments carried out in solid medium showed that A. niger did not showed mycelial growthin the presence of 1.5% calcium propionate and copper, while with potassium sorbate the growth was not significant. In cytotoxicity assay, copperpresented total inhibition and all nauplii died, followed by propionate y sorbate. These results shows that the additives are effective in fungi controllingin concentrations higher than those allowed by the argentine food code and they are cytotoxic, so it is important to look for other additives moreeffective and less toxic alternative.
Fil: Isaguirre, Andrea Celeste. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Santos, Rocío. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina
Fil: Quiroga Aromataris, Santiago Gabriel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina
XLII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo - Materia
-
Penicillium
Aspergillus
PROPIONATO
SORBATO - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/275897
Ver los metadatos del registro completo
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Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industryIsaguirre, Andrea CelesteSantos, RocíoQuiroga Aromataris, Santiago GabrielAmieva, María ItatíDelfini, Claudio DanielVillegas, Liliana BeatrizPenicilliumAspergillusPROPIONATOSORBATOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Food losses due to the presence of microorganisms, such as fungi, are a major concern worldwide, especially with a constantly growing worldpopulation. The development of these organisms leads to sensory defects in food ranging from visual deterioration to noticeable changes in smell, tasteor texture, but they can also have negative impacts on health due to the production of mycotoxins by some species. The objective of this work was toevaluate antifungal activity on fungi of the genus Aspergillus in the presence of Cu, Calcium Propionate and Potassium Sorbate, which are used asantifungals in agriculture and the food industry. The cytotoxicity also was evaluated. Fungi isolated and identified in previous work from spoiled breadwere used: Aspergillus niger and A. tritici. The proposed methodology included different concentrations of sodium propionate and sorbate (0.15?2 %w/v) in the presence of the microorganism under study in liquid Luria-Bertani (LB) culture medium. On the other hand, the antifungal activity ofdifferent additives was determined by measuring mycelial growth on extract-glucose agar (EG), with and without the additives. For the copper effect,a similar methodology with 400 ppm. For the cytotoxicity assay, Artemia salina eggs were incubated in saltwater at 27°C for 48 hours until the naupliihatched. The nauplii were exposed to the additive solution. The results showed inhibition of the growth of both fungi species with the different additivessolutions used. Propionate inhibited the growth of A. niger at a concentration of 2%, while for A. tritici was more resistant but a decrease in growthwas observed, compared to the positive control. With potassium sorbate it was observed that at concentrations of 0.5% and 1% no growth of A. nigerand A. tritici was observed, respectively. Finally, the experiments carried out in solid medium showed that A. niger did not showed mycelial growthin the presence of 1.5% calcium propionate and copper, while with potassium sorbate the growth was not significant. In cytotoxicity assay, copperpresented total inhibition and all nauplii died, followed by propionate y sorbate. These results shows that the additives are effective in fungi controllingin concentrations higher than those allowed by the argentine food code and they are cytotoxic, so it is important to look for other additives moreeffective and less toxic alternative.Fil: Isaguirre, Andrea Celeste. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Santos, Rocío. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; ArgentinaFil: Quiroga Aromataris, Santiago Gabriel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; ArgentinaFil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; ArgentinaXLII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275897Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry; XLII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2024; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:42:40Zoai:ri.conicet.gov.ar:11336/275897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:42:41.047CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| title |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| spellingShingle |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry Isaguirre, Andrea Celeste Penicillium Aspergillus PROPIONATO SORBATO |
| title_short |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| title_full |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| title_fullStr |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| title_full_unstemmed |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| title_sort |
Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry |
| dc.creator.none.fl_str_mv |
Isaguirre, Andrea Celeste Santos, Rocío Quiroga Aromataris, Santiago Gabriel Amieva, María Itatí Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author |
Isaguirre, Andrea Celeste |
| author_facet |
Isaguirre, Andrea Celeste Santos, Rocío Quiroga Aromataris, Santiago Gabriel Amieva, María Itatí Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author_role |
author |
| author2 |
Santos, Rocío Quiroga Aromataris, Santiago Gabriel Amieva, María Itatí Delfini, Claudio Daniel Villegas, Liliana Beatriz |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Penicillium Aspergillus PROPIONATO SORBATO |
| topic |
Penicillium Aspergillus PROPIONATO SORBATO |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Food losses due to the presence of microorganisms, such as fungi, are a major concern worldwide, especially with a constantly growing worldpopulation. The development of these organisms leads to sensory defects in food ranging from visual deterioration to noticeable changes in smell, tasteor texture, but they can also have negative impacts on health due to the production of mycotoxins by some species. The objective of this work was toevaluate antifungal activity on fungi of the genus Aspergillus in the presence of Cu, Calcium Propionate and Potassium Sorbate, which are used asantifungals in agriculture and the food industry. The cytotoxicity also was evaluated. Fungi isolated and identified in previous work from spoiled breadwere used: Aspergillus niger and A. tritici. The proposed methodology included different concentrations of sodium propionate and sorbate (0.15?2 %w/v) in the presence of the microorganism under study in liquid Luria-Bertani (LB) culture medium. On the other hand, the antifungal activity ofdifferent additives was determined by measuring mycelial growth on extract-glucose agar (EG), with and without the additives. For the copper effect,a similar methodology with 400 ppm. For the cytotoxicity assay, Artemia salina eggs were incubated in saltwater at 27°C for 48 hours until the naupliihatched. The nauplii were exposed to the additive solution. The results showed inhibition of the growth of both fungi species with the different additivessolutions used. Propionate inhibited the growth of A. niger at a concentration of 2%, while for A. tritici was more resistant but a decrease in growthwas observed, compared to the positive control. With potassium sorbate it was observed that at concentrations of 0.5% and 1% no growth of A. nigerand A. tritici was observed, respectively. Finally, the experiments carried out in solid medium showed that A. niger did not showed mycelial growthin the presence of 1.5% calcium propionate and copper, while with potassium sorbate the growth was not significant. In cytotoxicity assay, copperpresented total inhibition and all nauplii died, followed by propionate y sorbate. These results shows that the additives are effective in fungi controllingin concentrations higher than those allowed by the argentine food code and they are cytotoxic, so it is important to look for other additives moreeffective and less toxic alternative. Fil: Isaguirre, Andrea Celeste. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Santos, Rocío. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina Fil: Quiroga Aromataris, Santiago Gabriel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Cs.biológicas. Area Bioquímica Avanzada; Argentina XLII Reunión Científica Anual de la Sociedad de Biología de Cuyo San Luis Argentina Sociedad de Biología de Cuyo |
| description |
Food losses due to the presence of microorganisms, such as fungi, are a major concern worldwide, especially with a constantly growing worldpopulation. The development of these organisms leads to sensory defects in food ranging from visual deterioration to noticeable changes in smell, tasteor texture, but they can also have negative impacts on health due to the production of mycotoxins by some species. The objective of this work was toevaluate antifungal activity on fungi of the genus Aspergillus in the presence of Cu, Calcium Propionate and Potassium Sorbate, which are used asantifungals in agriculture and the food industry. The cytotoxicity also was evaluated. Fungi isolated and identified in previous work from spoiled breadwere used: Aspergillus niger and A. tritici. The proposed methodology included different concentrations of sodium propionate and sorbate (0.15?2 %w/v) in the presence of the microorganism under study in liquid Luria-Bertani (LB) culture medium. On the other hand, the antifungal activity ofdifferent additives was determined by measuring mycelial growth on extract-glucose agar (EG), with and without the additives. For the copper effect,a similar methodology with 400 ppm. For the cytotoxicity assay, Artemia salina eggs were incubated in saltwater at 27°C for 48 hours until the naupliihatched. The nauplii were exposed to the additive solution. The results showed inhibition of the growth of both fungi species with the different additivessolutions used. Propionate inhibited the growth of A. niger at a concentration of 2%, while for A. tritici was more resistant but a decrease in growthwas observed, compared to the positive control. With potassium sorbate it was observed that at concentrations of 0.5% and 1% no growth of A. nigerand A. tritici was observed, respectively. Finally, the experiments carried out in solid medium showed that A. niger did not showed mycelial growthin the presence of 1.5% calcium propionate and copper, while with potassium sorbate the growth was not significant. In cytotoxicity assay, copperpresented total inhibition and all nauplii died, followed by propionate y sorbate. These results shows that the additives are effective in fungi controllingin concentrations higher than those allowed by the argentine food code and they are cytotoxic, so it is important to look for other additives moreeffective and less toxic alternative. |
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2024 |
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Evaluation of antifungal activity and cytotoxicity of additives used in agriculture and the food industry; XLII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2024; 1-2 CONICET Digital CONICET |
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