Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons

Autores
Farias, Ginny G.; Valles, Ana Sofia; Colombres, Marcela; Godoy, Juan A.; Toledo, Enrique M.; Lukas, Ronald J.; Barrantes, Francisco Jose; Inestrosa, Nibaldo C.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nicotinic acetylcholine receptors (nAChRs) contribute significantly to hippocampal function. Alpha7-nAChRs are present in presynaptic sites in hippocampal neurons and may influence transmitter release, but the factors that determine their presynaptic localization are unknown. We report here that Wnt-7a, a ligand active in the canonical Wnt signaling pathway, induces dissociation of the adenomatous polyposis coli (APC) protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha7-nAChRs in hippocampal neurons. Interestingly, Wnt-7a induces the relocalization of APC to membranes, clustering of APC in neurites, and coclustering of APC with different, presynaptic protein markers. Wnt-7a also increases the number and size of coclusters of alpha7-nAChRs and APC in presynaptic terminals. These short-term changes in alpha7-nAChRs occur in the few minutes after ligand exposure and involve translocation to the plasma membrane without affecting total receptor levels. Longer-term exposure to Wnt-7a increases nAChR alpha7 subunit levels in an APC-independent manner and increases clusters of alpha7-nAChRs in neurites via an APC-dependent process. Together, these results demonstrate that stimulation through the canonical Wnt pathway regulates the presynaptic localization of APC and alpha7-nAChRs with APC serving as an intermediary in the alpha7-nAChR relocalization process. Modulation by Wnt signaling may be essential for alpha7-nAChR expression and function in synapses.
Fil: Farias, Ginny G.. Pontificia Universidad Católica de Chile; Chile
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colombres, Marcela. Pontificia Universidad Católica de Chile; Chile
Fil: Godoy, Juan A.. Pontificia Universidad Católica de Chile; Chile
Fil: Toledo, Enrique M.. Pontificia Universidad Católica de Chile; Chile
Fil: Lukas, Ronald J.. Barrow Neurological Institute; Estados Unidos
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; Chile
Materia
Nachr
Apc
Synapse
Wnt Target Gene
Neurons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43916
Acceder
id CONICETDig_582db67d2518c4f95d2d1277bde484df
oai_identifier_str oai:ri.conicet.gov.ar:11336/43916
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal NeuronsFarias, Ginny G.Valles, Ana SofiaColombres, MarcelaGodoy, Juan A.Toledo, Enrique M.Lukas, Ronald J.Barrantes, Francisco JoseInestrosa, Nibaldo C.NachrApcSynapseWnt Target GeneNeuronshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nicotinic acetylcholine receptors (nAChRs) contribute significantly to hippocampal function. Alpha7-nAChRs are present in presynaptic sites in hippocampal neurons and may influence transmitter release, but the factors that determine their presynaptic localization are unknown. We report here that Wnt-7a, a ligand active in the canonical Wnt signaling pathway, induces dissociation of the adenomatous polyposis coli (APC) protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha7-nAChRs in hippocampal neurons. Interestingly, Wnt-7a induces the relocalization of APC to membranes, clustering of APC in neurites, and coclustering of APC with different, presynaptic protein markers. Wnt-7a also increases the number and size of coclusters of alpha7-nAChRs and APC in presynaptic terminals. These short-term changes in alpha7-nAChRs occur in the few minutes after ligand exposure and involve translocation to the plasma membrane without affecting total receptor levels. Longer-term exposure to Wnt-7a increases nAChR alpha7 subunit levels in an APC-independent manner and increases clusters of alpha7-nAChRs in neurites via an APC-dependent process. Together, these results demonstrate that stimulation through the canonical Wnt pathway regulates the presynaptic localization of APC and alpha7-nAChRs with APC serving as an intermediary in the alpha7-nAChR relocalization process. Modulation by Wnt signaling may be essential for alpha7-nAChR expression and function in synapses.Fil: Farias, Ginny G.. Pontificia Universidad Católica de Chile; ChileFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Colombres, Marcela. Pontificia Universidad Católica de Chile; ChileFil: Godoy, Juan A.. Pontificia Universidad Católica de Chile; ChileFil: Toledo, Enrique M.. Pontificia Universidad Católica de Chile; ChileFil: Lukas, Ronald J.. Barrow Neurological Institute; Estados UnidosFil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; ChileSociety for Neuroscience2007-05-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43916Farias, Ginny G.; Valles, Ana Sofia; Colombres, Marcela; Godoy, Juan A.; Toledo, Enrique M.; et al.; Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons; Society for Neuroscience; Journal of Neuroscience; 27; 20; 16-5-2007; 5313-53250270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/27/20/5313.longinfo:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.3934-06.2007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:39Zoai:ri.conicet.gov.ar:11336/43916instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:39.535CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
title Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
spellingShingle Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
Farias, Ginny G.
Nachr
Apc
Synapse
Wnt Target Gene
Neurons
title_short Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
title_full Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
title_fullStr Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
title_full_unstemmed Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
title_sort Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons
dc.creator.none.fl_str_mv Farias, Ginny G.
Valles, Ana Sofia
Colombres, Marcela
Godoy, Juan A.
Toledo, Enrique M.
Lukas, Ronald J.
Barrantes, Francisco Jose
Inestrosa, Nibaldo C.
author Farias, Ginny G.
author_facet Farias, Ginny G.
Valles, Ana Sofia
Colombres, Marcela
Godoy, Juan A.
Toledo, Enrique M.
Lukas, Ronald J.
Barrantes, Francisco Jose
Inestrosa, Nibaldo C.
author_role author
author2 Valles, Ana Sofia
Colombres, Marcela
Godoy, Juan A.
Toledo, Enrique M.
Lukas, Ronald J.
Barrantes, Francisco Jose
Inestrosa, Nibaldo C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nachr
Apc
Synapse
Wnt Target Gene
Neurons
topic Nachr
Apc
Synapse
Wnt Target Gene
Neurons
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nicotinic acetylcholine receptors (nAChRs) contribute significantly to hippocampal function. Alpha7-nAChRs are present in presynaptic sites in hippocampal neurons and may influence transmitter release, but the factors that determine their presynaptic localization are unknown. We report here that Wnt-7a, a ligand active in the canonical Wnt signaling pathway, induces dissociation of the adenomatous polyposis coli (APC) protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha7-nAChRs in hippocampal neurons. Interestingly, Wnt-7a induces the relocalization of APC to membranes, clustering of APC in neurites, and coclustering of APC with different, presynaptic protein markers. Wnt-7a also increases the number and size of coclusters of alpha7-nAChRs and APC in presynaptic terminals. These short-term changes in alpha7-nAChRs occur in the few minutes after ligand exposure and involve translocation to the plasma membrane without affecting total receptor levels. Longer-term exposure to Wnt-7a increases nAChR alpha7 subunit levels in an APC-independent manner and increases clusters of alpha7-nAChRs in neurites via an APC-dependent process. Together, these results demonstrate that stimulation through the canonical Wnt pathway regulates the presynaptic localization of APC and alpha7-nAChRs with APC serving as an intermediary in the alpha7-nAChR relocalization process. Modulation by Wnt signaling may be essential for alpha7-nAChR expression and function in synapses.
Fil: Farias, Ginny G.. Pontificia Universidad Católica de Chile; Chile
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Colombres, Marcela. Pontificia Universidad Católica de Chile; Chile
Fil: Godoy, Juan A.. Pontificia Universidad Católica de Chile; Chile
Fil: Toledo, Enrique M.. Pontificia Universidad Católica de Chile; Chile
Fil: Lukas, Ronald J.. Barrow Neurological Institute; Estados Unidos
Fil: Barrantes, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Inestrosa, Nibaldo C.. Pontificia Universidad Católica de Chile; Chile
description Nicotinic acetylcholine receptors (nAChRs) contribute significantly to hippocampal function. Alpha7-nAChRs are present in presynaptic sites in hippocampal neurons and may influence transmitter release, but the factors that determine their presynaptic localization are unknown. We report here that Wnt-7a, a ligand active in the canonical Wnt signaling pathway, induces dissociation of the adenomatous polyposis coli (APC) protein from the beta-catenin cytoplasmic complex and the interaction of APC with alpha7-nAChRs in hippocampal neurons. Interestingly, Wnt-7a induces the relocalization of APC to membranes, clustering of APC in neurites, and coclustering of APC with different, presynaptic protein markers. Wnt-7a also increases the number and size of coclusters of alpha7-nAChRs and APC in presynaptic terminals. These short-term changes in alpha7-nAChRs occur in the few minutes after ligand exposure and involve translocation to the plasma membrane without affecting total receptor levels. Longer-term exposure to Wnt-7a increases nAChR alpha7 subunit levels in an APC-independent manner and increases clusters of alpha7-nAChRs in neurites via an APC-dependent process. Together, these results demonstrate that stimulation through the canonical Wnt pathway regulates the presynaptic localization of APC and alpha7-nAChRs with APC serving as an intermediary in the alpha7-nAChR relocalization process. Modulation by Wnt signaling may be essential for alpha7-nAChR expression and function in synapses.
publishDate 2007
dc.date.none.fl_str_mv 2007-05-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43916
Farias, Ginny G.; Valles, Ana Sofia; Colombres, Marcela; Godoy, Juan A.; Toledo, Enrique M.; et al.; Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons; Society for Neuroscience; Journal of Neuroscience; 27; 20; 16-5-2007; 5313-5325
0270-6474
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43916
identifier_str_mv Farias, Ginny G.; Valles, Ana Sofia; Colombres, Marcela; Godoy, Juan A.; Toledo, Enrique M.; et al.; Wnt-7a Induces Presynaptic Colocalization of  7-Nicotinic Acetylcholine Receptors and Adenomatous Polyposis Coli in Hippocampal Neurons; Society for Neuroscience; Journal of Neuroscience; 27; 20; 16-5-2007; 5313-5325
0270-6474
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/27/20/5313.long
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.3934-06.2007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269474978267136
score 13.13397

Publicaciones similares