Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni

Autores
Celaya, Liliana Soledad; Kolb Koslobsky, Nicolas
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Research background. The process for producing purified steviol glycosides from Stevia rebaudiana leaves (stevia) generally involves pretreatments, extraction, purification and crystallization. Pre-extraction or defatting can sometimes be a part of this process. It can remove impurities of low polarity, such as chlorophyll and fatty compounds. Nonpolar solvents can be used to defat stevia leaves. Experimental approach. In this study, we investigated ethyl acetate as a pre-extraction solvent for the defatting of dried and crushed stevia leaves. We compared pure ethyl acetate and water-saturated ethyl acetate as pre-extraction solvents in percolation extraction. We then evaluated the effects of pre-extraction on the concentration and purity of the extracts obtained with ethanol/water solvents. Results and conclusions. The recovery of nonpolar solvents was 2.3–3.9 % in pure ethyl acetate and 3.4–4.5 % in water-saturated ethyl acetate (from 40 to 60 °C). A low steviol glycoside loss can occur only with water-saturated ethyl acetate (on dry mass basis <0.5 %). In the Soxhlet extraction, the obtained yields were 8.43 with pure ethyl acetate and 10.44 % with water-saturated ethyl acetate. The steviol glycoside loss in the Soxhlet extraction was 10.70 % with water-saturated ethyl acetate. Defatted and non-defatted leaves were extracted with two ethanol/water solvents. Comparison of the results showed higher concentrations of glycoside in the pretreated leaves. Novelty and scientific contribution. The pre-extraction with ethyl acetate followed by the extraction with ethanol/water solvent lead to a higher concentration of steviol glycosides and a higher purity of the extracts. Ethyl acetate can be used as a pre-extraction solvent for the defatting of stevia leaves in the industrial production of this sweetener.
Fil: Celaya, Liliana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; Argentina
Fil: Kolb Koslobsky, Nicolas. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; Argentina
Materia
STEVIA
ETHIL ACETATE
DEFFATING
EXTRACT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/251257
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/251257
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana BertoniCelaya, Liliana SoledadKolb Koslobsky, NicolasSTEVIAETHIL ACETATEDEFFATINGEXTRACThttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Research background. The process for producing purified steviol glycosides from Stevia rebaudiana leaves (stevia) generally involves pretreatments, extraction, purification and crystallization. Pre-extraction or defatting can sometimes be a part of this process. It can remove impurities of low polarity, such as chlorophyll and fatty compounds. Nonpolar solvents can be used to defat stevia leaves. Experimental approach. In this study, we investigated ethyl acetate as a pre-extraction solvent for the defatting of dried and crushed stevia leaves. We compared pure ethyl acetate and water-saturated ethyl acetate as pre-extraction solvents in percolation extraction. We then evaluated the effects of pre-extraction on the concentration and purity of the extracts obtained with ethanol/water solvents. Results and conclusions. The recovery of nonpolar solvents was 2.3–3.9 % in pure ethyl acetate and 3.4–4.5 % in water-saturated ethyl acetate (from 40 to 60 °C). A low steviol glycoside loss can occur only with water-saturated ethyl acetate (on dry mass basis <0.5 %). In the Soxhlet extraction, the obtained yields were 8.43 with pure ethyl acetate and 10.44 % with water-saturated ethyl acetate. The steviol glycoside loss in the Soxhlet extraction was 10.70 % with water-saturated ethyl acetate. Defatted and non-defatted leaves were extracted with two ethanol/water solvents. Comparison of the results showed higher concentrations of glycoside in the pretreated leaves. Novelty and scientific contribution. The pre-extraction with ethyl acetate followed by the extraction with ethanol/water solvent lead to a higher concentration of steviol glycosides and a higher purity of the extracts. Ethyl acetate can be used as a pre-extraction solvent for the defatting of stevia leaves in the industrial production of this sweetener.Fil: Celaya, Liliana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; ArgentinaFil: Kolb Koslobsky, Nicolas. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; ArgentinaUniversity of Zagreb. Faculty of Food Technology and Biotechnology2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/251257Celaya, Liliana Soledad; Kolb Koslobsky, Nicolas; Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni; University of Zagreb. Faculty of Food Technology and Biotechnology; Food Technology and Biotechnology; 62; 3; 9-2024; 354-3601330-98621334-2606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ftb.com.hr/archives/1900-effect-of-ethyl-acetate-on-the-defatting-of-leaves-in-the-extraction-of-stevia-rebaudiana-bertoniinfo:eu-repo/semantics/altIdentifier/doi/10.17113/ftb.62.03.24.8492info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:18Zoai:ri.conicet.gov.ar:11336/251257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:18.656CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
title Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
spellingShingle Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
Celaya, Liliana Soledad
STEVIA
ETHIL ACETATE
DEFFATING
EXTRACT
title_short Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
title_full Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
title_fullStr Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
title_full_unstemmed Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
title_sort Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni
dc.creator.none.fl_str_mv Celaya, Liliana Soledad
Kolb Koslobsky, Nicolas
author Celaya, Liliana Soledad
author_facet Celaya, Liliana Soledad
Kolb Koslobsky, Nicolas
author_role author
author2 Kolb Koslobsky, Nicolas
author2_role author
dc.subject.none.fl_str_mv STEVIA
ETHIL ACETATE
DEFFATING
EXTRACT
topic STEVIA
ETHIL ACETATE
DEFFATING
EXTRACT
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Research background. The process for producing purified steviol glycosides from Stevia rebaudiana leaves (stevia) generally involves pretreatments, extraction, purification and crystallization. Pre-extraction or defatting can sometimes be a part of this process. It can remove impurities of low polarity, such as chlorophyll and fatty compounds. Nonpolar solvents can be used to defat stevia leaves. Experimental approach. In this study, we investigated ethyl acetate as a pre-extraction solvent for the defatting of dried and crushed stevia leaves. We compared pure ethyl acetate and water-saturated ethyl acetate as pre-extraction solvents in percolation extraction. We then evaluated the effects of pre-extraction on the concentration and purity of the extracts obtained with ethanol/water solvents. Results and conclusions. The recovery of nonpolar solvents was 2.3–3.9 % in pure ethyl acetate and 3.4–4.5 % in water-saturated ethyl acetate (from 40 to 60 °C). A low steviol glycoside loss can occur only with water-saturated ethyl acetate (on dry mass basis <0.5 %). In the Soxhlet extraction, the obtained yields were 8.43 with pure ethyl acetate and 10.44 % with water-saturated ethyl acetate. The steviol glycoside loss in the Soxhlet extraction was 10.70 % with water-saturated ethyl acetate. Defatted and non-defatted leaves were extracted with two ethanol/water solvents. Comparison of the results showed higher concentrations of glycoside in the pretreated leaves. Novelty and scientific contribution. The pre-extraction with ethyl acetate followed by the extraction with ethanol/water solvent lead to a higher concentration of steviol glycosides and a higher purity of the extracts. Ethyl acetate can be used as a pre-extraction solvent for the defatting of stevia leaves in the industrial production of this sweetener.
Fil: Celaya, Liliana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; Argentina
Fil: Kolb Koslobsky, Nicolas. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ingeniería Química; Argentina
description Research background. The process for producing purified steviol glycosides from Stevia rebaudiana leaves (stevia) generally involves pretreatments, extraction, purification and crystallization. Pre-extraction or defatting can sometimes be a part of this process. It can remove impurities of low polarity, such as chlorophyll and fatty compounds. Nonpolar solvents can be used to defat stevia leaves. Experimental approach. In this study, we investigated ethyl acetate as a pre-extraction solvent for the defatting of dried and crushed stevia leaves. We compared pure ethyl acetate and water-saturated ethyl acetate as pre-extraction solvents in percolation extraction. We then evaluated the effects of pre-extraction on the concentration and purity of the extracts obtained with ethanol/water solvents. Results and conclusions. The recovery of nonpolar solvents was 2.3–3.9 % in pure ethyl acetate and 3.4–4.5 % in water-saturated ethyl acetate (from 40 to 60 °C). A low steviol glycoside loss can occur only with water-saturated ethyl acetate (on dry mass basis <0.5 %). In the Soxhlet extraction, the obtained yields were 8.43 with pure ethyl acetate and 10.44 % with water-saturated ethyl acetate. The steviol glycoside loss in the Soxhlet extraction was 10.70 % with water-saturated ethyl acetate. Defatted and non-defatted leaves were extracted with two ethanol/water solvents. Comparison of the results showed higher concentrations of glycoside in the pretreated leaves. Novelty and scientific contribution. The pre-extraction with ethyl acetate followed by the extraction with ethanol/water solvent lead to a higher concentration of steviol glycosides and a higher purity of the extracts. Ethyl acetate can be used as a pre-extraction solvent for the defatting of stevia leaves in the industrial production of this sweetener.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/251257
Celaya, Liliana Soledad; Kolb Koslobsky, Nicolas; Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni; University of Zagreb. Faculty of Food Technology and Biotechnology; Food Technology and Biotechnology; 62; 3; 9-2024; 354-360
1330-9862
1334-2606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/251257
identifier_str_mv Celaya, Liliana Soledad; Kolb Koslobsky, Nicolas; Effect of Ethyl Acetate on the Defatting of Leaves in the Extraction of Stevia rebaudiana Bertoni; University of Zagreb. Faculty of Food Technology and Biotechnology; Food Technology and Biotechnology; 62; 3; 9-2024; 354-360
1330-9862
1334-2606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ftb.com.hr/archives/1900-effect-of-ethyl-acetate-on-the-defatting-of-leaves-in-the-extraction-of-stevia-rebaudiana-bertoni
info:eu-repo/semantics/altIdentifier/doi/10.17113/ftb.62.03.24.8492
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Zagreb. Faculty of Food Technology and Biotechnology
publisher.none.fl_str_mv University of Zagreb. Faculty of Food Technology and Biotechnology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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