Use of fish digestive extract for chicken feather degradation

Autores
Medina, Daiana Mailen; Leiva, Laura Cristina Ana; Van de Velde, Andrea Carolina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
By-products from animal sources are currently used for the benefit of providing added value to production. In the poultry industry, feathers represent up to 8.5% of chicken weight and constitute an important source of protein, mainly keratin. Due to the high aminoacids content, its processing to obtain hydrolysates becomes a commercial attraction. Besides, in the fishing industry, the viscera constitute 5% of the total weight of the fish and are a waste for the environment. They are considered an alternative source of enzymes (proteases) of high commercial value by their industrial and scientific applications. In the present work an enzymatic extract from fish pyloric cecae (Pygocentrus nattereri) was assayed on feathers in order to evaluate its capacity of hydrolyzes this keratin source under different medium contiditions. Preparations of extracts were made by mechanical digestion of tissues in buffer pH 7.8, 1:5 g tissue/ml, and then centrifuged. Proteolytic activity of fish enzymatic extract (FEE) was tested using a-Nbenzoyl-DL-arginine-p-nitroanilide. For the hydrolysis, first, feathers were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C. Second, 7.0 U/ml FEE was added (1:5) and incubated for 6 days at 37°C. After finishing hydrolysis stage, feathers were observed at optic microscope (OM) and sobrenadants were analyzed by UV spectra. Feathers were running in parallel under different treatments, such as absence of reducing agent, heat or FEE. OM analysis showed that FEE was capable of attack the feather structure, but the pre-treatment with 2-ME and heat increased the enzymatic action. UV210-310 nm analyses reveled a major increment of absorbance at 250-280 range in those samples from feathers treated with FEE, 2-ME and heat. Results demonstrate that FEE from P. nattereri is capable of degrade the native structure of feathers, yielding free molecules of hydrolyzed keratin. This property gives FEE a potential industrial use.
Fil: Medina, Daiana Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
Enzyme
Fish Pyloric Cecae
Keratin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181011
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Use of fish digestive extract for chicken feather degradationMedina, Daiana MailenLeiva, Laura Cristina AnaVan de Velde, Andrea CarolinaEnzymeFish Pyloric CecaeKeratinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1By-products from animal sources are currently used for the benefit of providing added value to production. In the poultry industry, feathers represent up to 8.5% of chicken weight and constitute an important source of protein, mainly keratin. Due to the high aminoacids content, its processing to obtain hydrolysates becomes a commercial attraction. Besides, in the fishing industry, the viscera constitute 5% of the total weight of the fish and are a waste for the environment. They are considered an alternative source of enzymes (proteases) of high commercial value by their industrial and scientific applications. In the present work an enzymatic extract from fish pyloric cecae (Pygocentrus nattereri) was assayed on feathers in order to evaluate its capacity of hydrolyzes this keratin source under different medium contiditions. Preparations of extracts were made by mechanical digestion of tissues in buffer pH 7.8, 1:5 g tissue/ml, and then centrifuged. Proteolytic activity of fish enzymatic extract (FEE) was tested using a-Nbenzoyl-DL-arginine-p-nitroanilide. For the hydrolysis, first, feathers were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C. Second, 7.0 U/ml FEE was added (1:5) and incubated for 6 days at 37°C. After finishing hydrolysis stage, feathers were observed at optic microscope (OM) and sobrenadants were analyzed by UV spectra. Feathers were running in parallel under different treatments, such as absence of reducing agent, heat or FEE. OM analysis showed that FEE was capable of attack the feather structure, but the pre-treatment with 2-ME and heat increased the enzymatic action. UV210-310 nm analyses reveled a major increment of absorbance at 250-280 range in those samples from feathers treated with FEE, 2-ME and heat. Results demonstrate that FEE from P. nattereri is capable of degrade the native structure of feathers, yielding free molecules of hydrolyzed keratin. This property gives FEE a potential industrial use.Fil: Medina, Daiana Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181011Use of fish digestive extract for chicken feather degradation; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 138-1380025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:18Zoai:ri.conicet.gov.ar:11336/181011instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:18.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of fish digestive extract for chicken feather degradation
title Use of fish digestive extract for chicken feather degradation
spellingShingle Use of fish digestive extract for chicken feather degradation
Medina, Daiana Mailen
Enzyme
Fish Pyloric Cecae
Keratin
title_short Use of fish digestive extract for chicken feather degradation
title_full Use of fish digestive extract for chicken feather degradation
title_fullStr Use of fish digestive extract for chicken feather degradation
title_full_unstemmed Use of fish digestive extract for chicken feather degradation
title_sort Use of fish digestive extract for chicken feather degradation
dc.creator.none.fl_str_mv Medina, Daiana Mailen
Leiva, Laura Cristina Ana
Van de Velde, Andrea Carolina
author Medina, Daiana Mailen
author_facet Medina, Daiana Mailen
Leiva, Laura Cristina Ana
Van de Velde, Andrea Carolina
author_role author
author2 Leiva, Laura Cristina Ana
Van de Velde, Andrea Carolina
author2_role author
author
dc.subject.none.fl_str_mv Enzyme
Fish Pyloric Cecae
Keratin
topic Enzyme
Fish Pyloric Cecae
Keratin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv By-products from animal sources are currently used for the benefit of providing added value to production. In the poultry industry, feathers represent up to 8.5% of chicken weight and constitute an important source of protein, mainly keratin. Due to the high aminoacids content, its processing to obtain hydrolysates becomes a commercial attraction. Besides, in the fishing industry, the viscera constitute 5% of the total weight of the fish and are a waste for the environment. They are considered an alternative source of enzymes (proteases) of high commercial value by their industrial and scientific applications. In the present work an enzymatic extract from fish pyloric cecae (Pygocentrus nattereri) was assayed on feathers in order to evaluate its capacity of hydrolyzes this keratin source under different medium contiditions. Preparations of extracts were made by mechanical digestion of tissues in buffer pH 7.8, 1:5 g tissue/ml, and then centrifuged. Proteolytic activity of fish enzymatic extract (FEE) was tested using a-Nbenzoyl-DL-arginine-p-nitroanilide. For the hydrolysis, first, feathers were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C. Second, 7.0 U/ml FEE was added (1:5) and incubated for 6 days at 37°C. After finishing hydrolysis stage, feathers were observed at optic microscope (OM) and sobrenadants were analyzed by UV spectra. Feathers were running in parallel under different treatments, such as absence of reducing agent, heat or FEE. OM analysis showed that FEE was capable of attack the feather structure, but the pre-treatment with 2-ME and heat increased the enzymatic action. UV210-310 nm analyses reveled a major increment of absorbance at 250-280 range in those samples from feathers treated with FEE, 2-ME and heat. Results demonstrate that FEE from P. nattereri is capable of degrade the native structure of feathers, yielding free molecules of hydrolyzed keratin. This property gives FEE a potential industrial use.
Fil: Medina, Daiana Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description By-products from animal sources are currently used for the benefit of providing added value to production. In the poultry industry, feathers represent up to 8.5% of chicken weight and constitute an important source of protein, mainly keratin. Due to the high aminoacids content, its processing to obtain hydrolysates becomes a commercial attraction. Besides, in the fishing industry, the viscera constitute 5% of the total weight of the fish and are a waste for the environment. They are considered an alternative source of enzymes (proteases) of high commercial value by their industrial and scientific applications. In the present work an enzymatic extract from fish pyloric cecae (Pygocentrus nattereri) was assayed on feathers in order to evaluate its capacity of hydrolyzes this keratin source under different medium contiditions. Preparations of extracts were made by mechanical digestion of tissues in buffer pH 7.8, 1:5 g tissue/ml, and then centrifuged. Proteolytic activity of fish enzymatic extract (FEE) was tested using a-Nbenzoyl-DL-arginine-p-nitroanilide. For the hydrolysis, first, feathers were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C. Second, 7.0 U/ml FEE was added (1:5) and incubated for 6 days at 37°C. After finishing hydrolysis stage, feathers were observed at optic microscope (OM) and sobrenadants were analyzed by UV spectra. Feathers were running in parallel under different treatments, such as absence of reducing agent, heat or FEE. OM analysis showed that FEE was capable of attack the feather structure, but the pre-treatment with 2-ME and heat increased the enzymatic action. UV210-310 nm analyses reveled a major increment of absorbance at 250-280 range in those samples from feathers treated with FEE, 2-ME and heat. Results demonstrate that FEE from P. nattereri is capable of degrade the native structure of feathers, yielding free molecules of hydrolyzed keratin. This property gives FEE a potential industrial use.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181011
Use of fish digestive extract for chicken feather degradation; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 138-138
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181011
identifier_str_mv Use of fish digestive extract for chicken feather degradation; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de ka Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 138-138
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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