Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus)
- Autores
- Lopez, Juan Marcelo; Gomez, Gabriela Noemi; Leiva, Laura Cristina Ana; Van de Velde, Andrea Carolina
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Hair is composed mainly of keratin (90%) is a fibrous and insoluble protein with high content of amino acid cysteine, responsible of disulfide bonds presence, which gives it high resistance to degradation. Hair structure consists of a medulla, cortex, and cuticle. This last cover the cortex, made of long filaments packed together (microfibrils). Currently, the industrial processing of animal sources generates significant volumes of highly polluting waste. The meat packaging and tannery industry, hairdressing salon, generate waste of keratinous nature. On the other hand, aquaculture discards considerable amounts of viscera, but these are considered an alternative source of enzymes with potentials industrial applications. Treatment of these protein-rich wastes is an attractive option that results in products with high added value. In the present study we evaluated the degradative capacity on hairs of a pyloric cecae extract from pacu (Piaractus mesopotamicus) under reducing and non-reducing conditions. The extract was prepared by mechanical digestion of pacu viscera in buffer pH 7.8, 1:5 g tissue/ml and proteolytic activity were assayed over Nα-Benzoyl-dl-arginine-p-nitroanilide as substrate. Hairs were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C, then 5.0 U/ml pacu extract was added (1:5) and incubated for 1h, 3h, 24h and 7 days at 37°C. Hairs were observed at scanning electron microscopy (SEM). Other samples of the same hair were exposed in parallel under different treatments, such as the absence of reducing agent, heat or fish extract. SEM analysis showed that viscera extract, only in presence of 2-ME and heat, was able of degrading the hair cuticle, exposing the cortex microfibrils just at the first day. Results demonstrate that pyloric cecae pacu extract is able to degrade hair pre-treated with heat and reducing agent, so this treatment could be used in the recovery of hair keratins.
Fil: Lopez, Juan Marcelo. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
Fil: Gomez, Gabriela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
Fil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina
LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LI Annual Meeting of Asociación Argentina de Farmacología Experimental (SAFE); XXI Annual Meeting of Sociedad Argentina de Biología (SAB); XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); IX Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio - Materia
-
Piaractus Mesopotamicus
Scanning Electron Microscopy
Pyloric Cecae Extract - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/153792
Ver los metadatos del registro completo
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Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus)Lopez, Juan MarceloGomez, Gabriela NoemiLeiva, Laura Cristina AnaVan de Velde, Andrea CarolinaPiaractus MesopotamicusScanning Electron MicroscopyPyloric Cecae Extracthttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Hair is composed mainly of keratin (90%) is a fibrous and insoluble protein with high content of amino acid cysteine, responsible of disulfide bonds presence, which gives it high resistance to degradation. Hair structure consists of a medulla, cortex, and cuticle. This last cover the cortex, made of long filaments packed together (microfibrils). Currently, the industrial processing of animal sources generates significant volumes of highly polluting waste. The meat packaging and tannery industry, hairdressing salon, generate waste of keratinous nature. On the other hand, aquaculture discards considerable amounts of viscera, but these are considered an alternative source of enzymes with potentials industrial applications. Treatment of these protein-rich wastes is an attractive option that results in products with high added value. In the present study we evaluated the degradative capacity on hairs of a pyloric cecae extract from pacu (Piaractus mesopotamicus) under reducing and non-reducing conditions. The extract was prepared by mechanical digestion of pacu viscera in buffer pH 7.8, 1:5 g tissue/ml and proteolytic activity were assayed over Nα-Benzoyl-dl-arginine-p-nitroanilide as substrate. Hairs were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C, then 5.0 U/ml pacu extract was added (1:5) and incubated for 1h, 3h, 24h and 7 days at 37°C. Hairs were observed at scanning electron microscopy (SEM). Other samples of the same hair were exposed in parallel under different treatments, such as the absence of reducing agent, heat or fish extract. SEM analysis showed that viscera extract, only in presence of 2-ME and heat, was able of degrading the hair cuticle, exposing the cortex microfibrils just at the first day. Results demonstrate that pyloric cecae pacu extract is able to degrade hair pre-treated with heat and reducing agent, so this treatment could be used in the recovery of hair keratins.Fil: Lopez, Juan Marcelo. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; ArgentinaFil: Gomez, Gabriela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; ArgentinaFil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; ArgentinaFil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; ArgentinaLXIV Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LI Annual Meeting of Asociación Argentina de Farmacología Experimental (SAFE); XXI Annual Meeting of Sociedad Argentina de Biología (SAB); XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); IX Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153792Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus); LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LI Annual Meeting of Asociación Argentina de Farmacología Experimental (SAFE); XXI Annual Meeting of Sociedad Argentina de Biología (SAB); XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); IX Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 11-110025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:28Zoai:ri.conicet.gov.ar:11336/153792instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:28.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| title |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| spellingShingle |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) Lopez, Juan Marcelo Piaractus Mesopotamicus Scanning Electron Microscopy Pyloric Cecae Extract |
| title_short |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| title_full |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| title_fullStr |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| title_full_unstemmed |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| title_sort |
Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus) |
| dc.creator.none.fl_str_mv |
Lopez, Juan Marcelo Gomez, Gabriela Noemi Leiva, Laura Cristina Ana Van de Velde, Andrea Carolina |
| author |
Lopez, Juan Marcelo |
| author_facet |
Lopez, Juan Marcelo Gomez, Gabriela Noemi Leiva, Laura Cristina Ana Van de Velde, Andrea Carolina |
| author_role |
author |
| author2 |
Gomez, Gabriela Noemi Leiva, Laura Cristina Ana Van de Velde, Andrea Carolina |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Piaractus Mesopotamicus Scanning Electron Microscopy Pyloric Cecae Extract |
| topic |
Piaractus Mesopotamicus Scanning Electron Microscopy Pyloric Cecae Extract |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Hair is composed mainly of keratin (90%) is a fibrous and insoluble protein with high content of amino acid cysteine, responsible of disulfide bonds presence, which gives it high resistance to degradation. Hair structure consists of a medulla, cortex, and cuticle. This last cover the cortex, made of long filaments packed together (microfibrils). Currently, the industrial processing of animal sources generates significant volumes of highly polluting waste. The meat packaging and tannery industry, hairdressing salon, generate waste of keratinous nature. On the other hand, aquaculture discards considerable amounts of viscera, but these are considered an alternative source of enzymes with potentials industrial applications. Treatment of these protein-rich wastes is an attractive option that results in products with high added value. In the present study we evaluated the degradative capacity on hairs of a pyloric cecae extract from pacu (Piaractus mesopotamicus) under reducing and non-reducing conditions. The extract was prepared by mechanical digestion of pacu viscera in buffer pH 7.8, 1:5 g tissue/ml and proteolytic activity were assayed over Nα-Benzoyl-dl-arginine-p-nitroanilide as substrate. Hairs were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C, then 5.0 U/ml pacu extract was added (1:5) and incubated for 1h, 3h, 24h and 7 days at 37°C. Hairs were observed at scanning electron microscopy (SEM). Other samples of the same hair were exposed in parallel under different treatments, such as the absence of reducing agent, heat or fish extract. SEM analysis showed that viscera extract, only in presence of 2-ME and heat, was able of degrading the hair cuticle, exposing the cortex microfibrils just at the first day. Results demonstrate that pyloric cecae pacu extract is able to degrade hair pre-treated with heat and reducing agent, so this treatment could be used in the recovery of hair keratins. Fil: Lopez, Juan Marcelo. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina Fil: Gomez, Gabriela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina Fil: Van de Velde, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica. Laboratorio de Investigación en Proteínas; Argentina LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LI Annual Meeting of Asociación Argentina de Farmacología Experimental (SAFE); XXI Annual Meeting of Sociedad Argentina de Biología (SAB); XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); IX Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL) Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Asociación Argentina de Farmacología Experimental Sociedad Argentina de Biología Sociedad Argentina de Protozoología Asociación Argentina de Nanomedicinas Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio |
| description |
Hair is composed mainly of keratin (90%) is a fibrous and insoluble protein with high content of amino acid cysteine, responsible of disulfide bonds presence, which gives it high resistance to degradation. Hair structure consists of a medulla, cortex, and cuticle. This last cover the cortex, made of long filaments packed together (microfibrils). Currently, the industrial processing of animal sources generates significant volumes of highly polluting waste. The meat packaging and tannery industry, hairdressing salon, generate waste of keratinous nature. On the other hand, aquaculture discards considerable amounts of viscera, but these are considered an alternative source of enzymes with potentials industrial applications. Treatment of these protein-rich wastes is an attractive option that results in products with high added value. In the present study we evaluated the degradative capacity on hairs of a pyloric cecae extract from pacu (Piaractus mesopotamicus) under reducing and non-reducing conditions. The extract was prepared by mechanical digestion of pacu viscera in buffer pH 7.8, 1:5 g tissue/ml and proteolytic activity were assayed over Nα-Benzoyl-dl-arginine-p-nitroanilide as substrate. Hairs were pre-treated with buffer 7.8, 1% 2-mercaptoethanol (2-ME), for 20 min at 100°C, then 5.0 U/ml pacu extract was added (1:5) and incubated for 1h, 3h, 24h and 7 days at 37°C. Hairs were observed at scanning electron microscopy (SEM). Other samples of the same hair were exposed in parallel under different treatments, such as the absence of reducing agent, heat or fish extract. SEM analysis showed that viscera extract, only in presence of 2-ME and heat, was able of degrading the hair cuticle, exposing the cortex microfibrils just at the first day. Results demonstrate that pyloric cecae pacu extract is able to degrade hair pre-treated with heat and reducing agent, so this treatment could be used in the recovery of hair keratins. |
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2019 |
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2019 |
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Scanning electron microscopy analysis of hair treated with pyloric cecae extract from pacu (Piaractus mesopotamicus); LXIV Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LI Annual Meeting of Asociación Argentina de Farmacología Experimental (SAFE); XXI Annual Meeting of Sociedad Argentina de Biología (SAB); XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); IX Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 11-11 0025-7680 1669-9106 CONICET Digital CONICET |
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eng |
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