Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium

Autores
Rios Cruz, Patricia Leonela; Bezus, Brenda; Cavalitto, Sebastián Fernando; Cavello, Ivana Alejandra
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results: In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h⁻¹). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml⁻¹) and keratinolytic (33.04 ± 1.09 U ml⁻¹) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion: We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Química
Cold active keratinase
Pedobacter
Keratin biodegradation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/154706
Acceder
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spelling Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacteriumRios Cruz, Patricia LeonelaBezus, BrendaCavalitto, Sebastián FernandoCavello, Ivana AlejandraCiencias ExactasQuímicaCold active keratinasePedobacterKeratin biodegradationBackground: Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results: In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h⁻¹). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml⁻¹) and keratinolytic (33.04 ± 1.09 U ml⁻¹) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion: We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive.Centro de Investigación y Desarrollo en Fermentaciones Industriales2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/154706enginfo:eu-repo/semantics/altIdentifier/issn/2090-5920info:eu-repo/semantics/altIdentifier/doi/10.1186/s43141-022-00356-xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:40:05Zoai:sedici.unlp.edu.ar:10915/154706Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:40:05.76SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
title Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
spellingShingle Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
Rios Cruz, Patricia Leonela
Ciencias Exactas
Química
Cold active keratinase
Pedobacter
Keratin biodegradation
title_short Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
title_full Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
title_fullStr Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
title_full_unstemmed Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
title_sort Production and characterization of a new detergent‑stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin‑degrading bacterium
dc.creator.none.fl_str_mv Rios Cruz, Patricia Leonela
Bezus, Brenda
Cavalitto, Sebastián Fernando
Cavello, Ivana Alejandra
author Rios Cruz, Patricia Leonela
author_facet Rios Cruz, Patricia Leonela
Bezus, Brenda
Cavalitto, Sebastián Fernando
Cavello, Ivana Alejandra
author_role author
author2 Bezus, Brenda
Cavalitto, Sebastián Fernando
Cavello, Ivana Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Cold active keratinase
Pedobacter
Keratin biodegradation
topic Ciencias Exactas
Química
Cold active keratinase
Pedobacter
Keratin biodegradation
dc.description.none.fl_txt_mv Background: Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results: In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h⁻¹). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml⁻¹) and keratinolytic (33.04 ± 1.09 U ml⁻¹) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion: We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Background: Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results: In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h⁻¹). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml⁻¹) and keratinolytic (33.04 ± 1.09 U ml⁻¹) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion: We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/154706
url http://sedici.unlp.edu.ar/handle/10915/154706
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2090-5920
info:eu-repo/semantics/altIdentifier/doi/10.1186/s43141-022-00356-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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instname_str Universidad Nacional de La Plata
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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