Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish

Autores
Friedman, Ivana Soledad; Fernandez Gimenez, Analia Veronica; Contreras, Edgardo Martin
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, the fishing industry is highly developed, producing large quantities of waste products from fish processing which contain digestive enzymes with potential biotechnological uses. Among these enzymes are proteinases that hydrolyze proteins and can convert them into functional ingredients. To use these value-added compounds in industrial processes, kinetic characterization of these proteinases under specific conditions, such as contact time, pH, and temperature, must first be carried out. The objective of the present work was to perform a kinetic characterization of crude extracts of alkaline proteinases from intestine-ceca of Merluccius hubbsi, Percophis brasiliensis, Urophycis brasiliensis, and Cynoscion guatucupa. Results showed that the reached maximum values of A440 depended on both the species analyzed and the initial concentration of azocasein. The maximum concentration of hydrolysis products obtained at long times was proportional to the initial concentration of the target protein. P. brasiliensis was the most suitable tested species to obtain high hydrolysis rates at high azocasein concentrations, and exhibited the highest proteinase activities, so it can be considered a candidate species for future biotechnological applications.
Fil: Friedman, Ivana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
DIGESTIVE PROTEINASES
FISH
KINETIC MODELING
WASTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221418
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fishFriedman, Ivana SoledadFernandez Gimenez, Analia VeronicaContreras, Edgardo MartinDIGESTIVE PROTEINASESFISHKINETIC MODELINGWASTEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Argentina, the fishing industry is highly developed, producing large quantities of waste products from fish processing which contain digestive enzymes with potential biotechnological uses. Among these enzymes are proteinases that hydrolyze proteins and can convert them into functional ingredients. To use these value-added compounds in industrial processes, kinetic characterization of these proteinases under specific conditions, such as contact time, pH, and temperature, must first be carried out. The objective of the present work was to perform a kinetic characterization of crude extracts of alkaline proteinases from intestine-ceca of Merluccius hubbsi, Percophis brasiliensis, Urophycis brasiliensis, and Cynoscion guatucupa. Results showed that the reached maximum values of A440 depended on both the species analyzed and the initial concentration of azocasein. The maximum concentration of hydrolysis products obtained at long times was proportional to the initial concentration of the target protein. P. brasiliensis was the most suitable tested species to obtain high hydrolysis rates at high azocasein concentrations, and exhibited the highest proteinase activities, so it can be considered a candidate species for future biotechnological applications.Fil: Friedman, Ivana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaElsevier2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221418Friedman, Ivana Soledad; Fernandez Gimenez, Analia Veronica; Contreras, Edgardo Martin; Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish; Elsevier; Bioresource Technology Reports; 23; 7-2023; 1-302589-014XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2589014X23002347info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biteb.2023.101563info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:23Zoai:ri.conicet.gov.ar:11336/221418instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:23.494CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
title Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
spellingShingle Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
Friedman, Ivana Soledad
DIGESTIVE PROTEINASES
FISH
KINETIC MODELING
WASTE
title_short Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
title_full Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
title_fullStr Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
title_full_unstemmed Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
title_sort Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish
dc.creator.none.fl_str_mv Friedman, Ivana Soledad
Fernandez Gimenez, Analia Veronica
Contreras, Edgardo Martin
author Friedman, Ivana Soledad
author_facet Friedman, Ivana Soledad
Fernandez Gimenez, Analia Veronica
Contreras, Edgardo Martin
author_role author
author2 Fernandez Gimenez, Analia Veronica
Contreras, Edgardo Martin
author2_role author
author
dc.subject.none.fl_str_mv DIGESTIVE PROTEINASES
FISH
KINETIC MODELING
WASTE
topic DIGESTIVE PROTEINASES
FISH
KINETIC MODELING
WASTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Argentina, the fishing industry is highly developed, producing large quantities of waste products from fish processing which contain digestive enzymes with potential biotechnological uses. Among these enzymes are proteinases that hydrolyze proteins and can convert them into functional ingredients. To use these value-added compounds in industrial processes, kinetic characterization of these proteinases under specific conditions, such as contact time, pH, and temperature, must first be carried out. The objective of the present work was to perform a kinetic characterization of crude extracts of alkaline proteinases from intestine-ceca of Merluccius hubbsi, Percophis brasiliensis, Urophycis brasiliensis, and Cynoscion guatucupa. Results showed that the reached maximum values of A440 depended on both the species analyzed and the initial concentration of azocasein. The maximum concentration of hydrolysis products obtained at long times was proportional to the initial concentration of the target protein. P. brasiliensis was the most suitable tested species to obtain high hydrolysis rates at high azocasein concentrations, and exhibited the highest proteinase activities, so it can be considered a candidate species for future biotechnological applications.
Fil: Friedman, Ivana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description In Argentina, the fishing industry is highly developed, producing large quantities of waste products from fish processing which contain digestive enzymes with potential biotechnological uses. Among these enzymes are proteinases that hydrolyze proteins and can convert them into functional ingredients. To use these value-added compounds in industrial processes, kinetic characterization of these proteinases under specific conditions, such as contact time, pH, and temperature, must first be carried out. The objective of the present work was to perform a kinetic characterization of crude extracts of alkaline proteinases from intestine-ceca of Merluccius hubbsi, Percophis brasiliensis, Urophycis brasiliensis, and Cynoscion guatucupa. Results showed that the reached maximum values of A440 depended on both the species analyzed and the initial concentration of azocasein. The maximum concentration of hydrolysis products obtained at long times was proportional to the initial concentration of the target protein. P. brasiliensis was the most suitable tested species to obtain high hydrolysis rates at high azocasein concentrations, and exhibited the highest proteinase activities, so it can be considered a candidate species for future biotechnological applications.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221418
Friedman, Ivana Soledad; Fernandez Gimenez, Analia Veronica; Contreras, Edgardo Martin; Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish; Elsevier; Bioresource Technology Reports; 23; 7-2023; 1-30
2589-014X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221418
identifier_str_mv Friedman, Ivana Soledad; Fernandez Gimenez, Analia Veronica; Contreras, Edgardo Martin; Kinetic characterization of digestive proteinases extracted from the processing waste of South Atlantic fish; Elsevier; Bioresource Technology Reports; 23; 7-2023; 1-30
2589-014X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2589014X23002347
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biteb.2023.101563
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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