The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums

Autores
Ragone, María Inés; Sella, Mariana; Conforti, Paula Andrea; Volonté, María G.; Consolini, Alicia Elvira
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The spasmolytic effects of an acqueous extract of cedrón (AEC) were studied on rat isolated duodenums. This plant (Aloysia citriodora Palau, Verbenaceae) is widely used for gastrointestinal disorders and as eupeptic in South America. AEC non-competitively inhibited the dose–response curve (DRC) of Ach (IC50 of 1.34 ± 0.49 mg lyophilized/mL) and the DRC of Ca2+ in high-[K2−]o (IC50 of 2.64 ± 0.23 mg/mL). AEC potentiated the non-competitive inhibition of either 30 μmol/L W-7 (a calmodulin blocker) and 5–15 μmol/L papaverine on the Ca2+-DRC. Also, AEC relaxed the contracture produced by high-[K+]o (IC50 of 2.6 ± 0.2 mg/mL) until 81.0 ± 3.2% of the maximal effect of papaverine and 78.1 ± 5.0% of the quercetin, the most selective inhibitor of PDE. The AEC relaxation was non-competitively inhibited by 10–30 μmol/L methylene blue and competitively antagonized by 40 mmol/L TEA. The relaxation of 1 mg/mL AEC was inhibited by hypoxia, but not that of 2 mg/mL. Two flavonoids were identified by HPLC in the AEC: vitexin and isovitexin. Vitexin non-competitively inhibited the Ach-DRC ( of 5.7 ± 0.4) but significantly run leftward the DRC of Ca2+. Isovitexin did not significantly inhibit the DRC of Ach nor Ca2+. The results suggest that the spasmolytic effect of AEC could be mostly associated to the increase in cGMP (target shared with the PDE inhibitors) and the activation of K+-channels. At low concentrations, AEC also inhibits the aerobic metabolism. The flavonoid vitexin is partially responsible for the effect, since it non-competitively inhibits Ach but not the Ca2+ influx. Isovitexin was devoid of activity on duodenums.
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Sella, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Conforti, Paula Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Volonté, María G.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Control de Calidad de Medicamentos; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aloysia citriodora (Palau)
Verbenaceae
Rat duodenum
Antispasmodic
Vitexin
Isovitexin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110793
Acceder
id CONICETDig_2d05bf27126312924bb3eeca0409c9d7
oai_identifier_str oai:ri.conicet.gov.ar:11336/110793
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenumsRagone, María InésSella, MarianaConforti, Paula AndreaVolonté, María G.Consolini, Alicia ElviraAloysia citriodora (Palau)VerbenaceaeRat duodenumAntispasmodicVitexinIsovitexinhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The spasmolytic effects of an acqueous extract of cedrón (AEC) were studied on rat isolated duodenums. This plant (Aloysia citriodora Palau, Verbenaceae) is widely used for gastrointestinal disorders and as eupeptic in South America. AEC non-competitively inhibited the dose–response curve (DRC) of Ach (IC50 of 1.34 ± 0.49 mg lyophilized/mL) and the DRC of Ca2+ in high-[K2−]o (IC50 of 2.64 ± 0.23 mg/mL). AEC potentiated the non-competitive inhibition of either 30 μmol/L W-7 (a calmodulin blocker) and 5–15 μmol/L papaverine on the Ca2+-DRC. Also, AEC relaxed the contracture produced by high-[K+]o (IC50 of 2.6 ± 0.2 mg/mL) until 81.0 ± 3.2% of the maximal effect of papaverine and 78.1 ± 5.0% of the quercetin, the most selective inhibitor of PDE. The AEC relaxation was non-competitively inhibited by 10–30 μmol/L methylene blue and competitively antagonized by 40 mmol/L TEA. The relaxation of 1 mg/mL AEC was inhibited by hypoxia, but not that of 2 mg/mL. Two flavonoids were identified by HPLC in the AEC: vitexin and isovitexin. Vitexin non-competitively inhibited the Ach-DRC ( of 5.7 ± 0.4) but significantly run leftward the DRC of Ca2+. Isovitexin did not significantly inhibit the DRC of Ach nor Ca2+. The results suggest that the spasmolytic effect of AEC could be mostly associated to the increase in cGMP (target shared with the PDE inhibitors) and the activation of K+-channels. At low concentrations, AEC also inhibits the aerobic metabolism. The flavonoid vitexin is partially responsible for the effect, since it non-competitively inhibits Ach but not the Ca2+ influx. Isovitexin was devoid of activity on duodenums.Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Sella, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Conforti, Paula Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Volonté, María G.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Control de Calidad de Medicamentos; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2007-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110793Ragone, María Inés; Sella, Mariana; Conforti, Paula Andrea; Volonté, María G.; Consolini, Alicia Elvira; The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums; Elsevier Ireland; Journal of Ethnopharmacology; 113; 2; 9-2007; 258-2660378-8741CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378874107002814?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2007.06.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:08Zoai:ri.conicet.gov.ar:11336/110793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:08.798CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
title The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
spellingShingle The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
Ragone, María Inés
Aloysia citriodora (Palau)
Verbenaceae
Rat duodenum
Antispasmodic
Vitexin
Isovitexin
title_short The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
title_full The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
title_fullStr The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
title_full_unstemmed The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
title_sort The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums
dc.creator.none.fl_str_mv Ragone, María Inés
Sella, Mariana
Conforti, Paula Andrea
Volonté, María G.
Consolini, Alicia Elvira
author Ragone, María Inés
author_facet Ragone, María Inés
Sella, Mariana
Conforti, Paula Andrea
Volonté, María G.
Consolini, Alicia Elvira
author_role author
author2 Sella, Mariana
Conforti, Paula Andrea
Volonté, María G.
Consolini, Alicia Elvira
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aloysia citriodora (Palau)
Verbenaceae
Rat duodenum
Antispasmodic
Vitexin
Isovitexin
topic Aloysia citriodora (Palau)
Verbenaceae
Rat duodenum
Antispasmodic
Vitexin
Isovitexin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The spasmolytic effects of an acqueous extract of cedrón (AEC) were studied on rat isolated duodenums. This plant (Aloysia citriodora Palau, Verbenaceae) is widely used for gastrointestinal disorders and as eupeptic in South America. AEC non-competitively inhibited the dose–response curve (DRC) of Ach (IC50 of 1.34 ± 0.49 mg lyophilized/mL) and the DRC of Ca2+ in high-[K2−]o (IC50 of 2.64 ± 0.23 mg/mL). AEC potentiated the non-competitive inhibition of either 30 μmol/L W-7 (a calmodulin blocker) and 5–15 μmol/L papaverine on the Ca2+-DRC. Also, AEC relaxed the contracture produced by high-[K+]o (IC50 of 2.6 ± 0.2 mg/mL) until 81.0 ± 3.2% of the maximal effect of papaverine and 78.1 ± 5.0% of the quercetin, the most selective inhibitor of PDE. The AEC relaxation was non-competitively inhibited by 10–30 μmol/L methylene blue and competitively antagonized by 40 mmol/L TEA. The relaxation of 1 mg/mL AEC was inhibited by hypoxia, but not that of 2 mg/mL. Two flavonoids were identified by HPLC in the AEC: vitexin and isovitexin. Vitexin non-competitively inhibited the Ach-DRC ( of 5.7 ± 0.4) but significantly run leftward the DRC of Ca2+. Isovitexin did not significantly inhibit the DRC of Ach nor Ca2+. The results suggest that the spasmolytic effect of AEC could be mostly associated to the increase in cGMP (target shared with the PDE inhibitors) and the activation of K+-channels. At low concentrations, AEC also inhibits the aerobic metabolism. The flavonoid vitexin is partially responsible for the effect, since it non-competitively inhibits Ach but not the Ca2+ influx. Isovitexin was devoid of activity on duodenums.
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Sella, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Fil: Conforti, Paula Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Volonté, María G.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Control de Calidad de Medicamentos; Argentina
Fil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The spasmolytic effects of an acqueous extract of cedrón (AEC) were studied on rat isolated duodenums. This plant (Aloysia citriodora Palau, Verbenaceae) is widely used for gastrointestinal disorders and as eupeptic in South America. AEC non-competitively inhibited the dose–response curve (DRC) of Ach (IC50 of 1.34 ± 0.49 mg lyophilized/mL) and the DRC of Ca2+ in high-[K2−]o (IC50 of 2.64 ± 0.23 mg/mL). AEC potentiated the non-competitive inhibition of either 30 μmol/L W-7 (a calmodulin blocker) and 5–15 μmol/L papaverine on the Ca2+-DRC. Also, AEC relaxed the contracture produced by high-[K+]o (IC50 of 2.6 ± 0.2 mg/mL) until 81.0 ± 3.2% of the maximal effect of papaverine and 78.1 ± 5.0% of the quercetin, the most selective inhibitor of PDE. The AEC relaxation was non-competitively inhibited by 10–30 μmol/L methylene blue and competitively antagonized by 40 mmol/L TEA. The relaxation of 1 mg/mL AEC was inhibited by hypoxia, but not that of 2 mg/mL. Two flavonoids were identified by HPLC in the AEC: vitexin and isovitexin. Vitexin non-competitively inhibited the Ach-DRC ( of 5.7 ± 0.4) but significantly run leftward the DRC of Ca2+. Isovitexin did not significantly inhibit the DRC of Ach nor Ca2+. The results suggest that the spasmolytic effect of AEC could be mostly associated to the increase in cGMP (target shared with the PDE inhibitors) and the activation of K+-channels. At low concentrations, AEC also inhibits the aerobic metabolism. The flavonoid vitexin is partially responsible for the effect, since it non-competitively inhibits Ach but not the Ca2+ influx. Isovitexin was devoid of activity on duodenums.
publishDate 2007
dc.date.none.fl_str_mv 2007-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110793
Ragone, María Inés; Sella, Mariana; Conforti, Paula Andrea; Volonté, María G.; Consolini, Alicia Elvira; The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums; Elsevier Ireland; Journal of Ethnopharmacology; 113; 2; 9-2007; 258-266
0378-8741
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110793
identifier_str_mv Ragone, María Inés; Sella, Mariana; Conforti, Paula Andrea; Volonté, María G.; Consolini, Alicia Elvira; The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums; Elsevier Ireland; Journal of Ethnopharmacology; 113; 2; 9-2007; 258-266
0378-8741
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378874107002814?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2007.06.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613573280530432
score 13.070432

Publicaciones similares