Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by ace...
- Autores
- Consolini, Alicia E.; Berardi, Andrea Beatriz; Rosella, María Adelaida; Volonté, María Guillermina
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The antispasmodic effects of acqueous extracts (AE) and tinctures (T) of Aloysia polystachya (Griseb.) Moldenke and Aloysia gratissima (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of A. polystachya and A. gratissima respectively inhibited the ACh-DRC at the IC50 of 3.15±0.57 and 6.46±2.28 mg leaves/mL. The Ca2+-antagonist activity of both T occurred with IC50 respectively similar to those of the ACh-DRC, and was potentiated by the depolarization produced by 10 mM TEA, a blocker of K+-channels. The spasmolytic effect of T does not involve DA release and binding to D2, since it was not reduced by 10 μM metoclopramide. Also, T induced dose-dependent relaxation on the tonic contracture produced by high-[K+]o and ACh. By TLC there were detected in the leaves the presence of carvone, and flavonoids such as quercetin and hesperidin. By HPLC there were not found vitexin nor isovitexin, identified in A. citriodora. The monoterpene (-)-carvone non-competitively inhibited the ACh-DRC (pD ́2 of 4.0±0.1) and the DRC of Ca2+ (pD ́2 of 3.86±0.19), suggesting that the Ca2+-influx blockade is the mechanism of its antispasmodic effect. Results suggest that the antispasmodic effect of A. polystachya and A. gratissima are mostly explained by the non-competitive blockade of Ca+2 influx. It could be associated to the presence of flavonoids, and in the tinctures to some spasmolytic components of the essential oil such as carvone.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Farmacia
acetylcholine
aloysia
calcium
antispasmodic
Ca antagonist
carvone
verbenaceae
hesperidin
isovitexin
quercetin
depolarization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/3.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/37521
Ver los metadatos del registro completo
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Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calciumConsolini, Alicia E.Berardi, Andrea BeatrizRosella, María AdelaidaVolonté, María GuillerminaCiencias ExactasFarmaciaacetylcholinealoysiacalciumantispasmodicCa antagonistcarvoneverbenaceaehesperidinisovitexinquercetindepolarizationThe antispasmodic effects of acqueous extracts (AE) and tinctures (T) of <i>Aloysia polystachya</i> (Griseb.) Moldenke and <i>Aloysia gratissima</i> (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of <i>A. polystachya</i> and <i>A. gratissima</i> respectively inhibited the ACh-DRC at the IC50 of 3.15±0.57 and 6.46±2.28 mg leaves/mL. The Ca2+-antagonist activity of both T occurred with IC50 respectively similar to those of the ACh-DRC, and was potentiated by the depolarization produced by 10 mM TEA, a blocker of K+-channels. The spasmolytic effect of T does not involve DA release and binding to D2, since it was not reduced by 10 μM metoclopramide. Also, T induced dose-dependent relaxation on the tonic contracture produced by high-[K+]o and ACh. By TLC there were detected in the leaves the presence of carvone, and flavonoids such as quercetin and hesperidin. By HPLC there were not found vitexin nor isovitexin, identified in <i>A. citriodora</i>. The monoterpene (-)-carvone non-competitively inhibited the ACh-DRC (pD ́2 of 4.0±0.1) and the DRC of Ca2+ (pD ́2 of 3.86±0.19), suggesting that the Ca2+-influx blockade is the mechanism of its antispasmodic effect. Results suggest that the antispasmodic effect of <i>A. polystachya</i> and <i>A. gratissima</i> are mostly explained by the non-competitive blockade of Ca+2 influx. It could be associated to the presence of flavonoids, and in the tinctures to some spasmolytic components of the essential oil such as carvone.Facultad de Ciencias Exactas2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf889-900http://sedici.unlp.edu.ar/handle/10915/37521enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/pdf/rbfar/v21n5/aop12811.pdfinfo:eu-repo/semantics/altIdentifier/issn/0102-695Xinfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0102-695X2011005000137info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T10:57:03Zoai:sedici.unlp.edu.ar:10915/37521Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 10:57:04.214SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| title |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| spellingShingle |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium Consolini, Alicia E. Ciencias Exactas Farmacia acetylcholine aloysia calcium antispasmodic Ca antagonist carvone verbenaceae hesperidin isovitexin quercetin depolarization |
| title_short |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| title_full |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| title_fullStr |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| title_full_unstemmed |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| title_sort |
Antispasmodic effects of <i>Aloysia polystachya</i> and <i>A. gratissima</i> tinctures and extracts are due to non-competitive inhibition of intestinal contractility induced by acethylcholine and calcium |
| dc.creator.none.fl_str_mv |
Consolini, Alicia E. Berardi, Andrea Beatriz Rosella, María Adelaida Volonté, María Guillermina |
| author |
Consolini, Alicia E. |
| author_facet |
Consolini, Alicia E. Berardi, Andrea Beatriz Rosella, María Adelaida Volonté, María Guillermina |
| author_role |
author |
| author2 |
Berardi, Andrea Beatriz Rosella, María Adelaida Volonté, María Guillermina |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Farmacia acetylcholine aloysia calcium antispasmodic Ca antagonist carvone verbenaceae hesperidin isovitexin quercetin depolarization |
| topic |
Ciencias Exactas Farmacia acetylcholine aloysia calcium antispasmodic Ca antagonist carvone verbenaceae hesperidin isovitexin quercetin depolarization |
| dc.description.none.fl_txt_mv |
The antispasmodic effects of acqueous extracts (AE) and tinctures (T) of <i>Aloysia polystachya</i> (Griseb.) Moldenke and <i>Aloysia gratissima</i> (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of <i>A. polystachya</i> and <i>A. gratissima</i> respectively inhibited the ACh-DRC at the IC50 of 3.15±0.57 and 6.46±2.28 mg leaves/mL. The Ca2+-antagonist activity of both T occurred with IC50 respectively similar to those of the ACh-DRC, and was potentiated by the depolarization produced by 10 mM TEA, a blocker of K+-channels. The spasmolytic effect of T does not involve DA release and binding to D2, since it was not reduced by 10 μM metoclopramide. Also, T induced dose-dependent relaxation on the tonic contracture produced by high-[K+]o and ACh. By TLC there were detected in the leaves the presence of carvone, and flavonoids such as quercetin and hesperidin. By HPLC there were not found vitexin nor isovitexin, identified in <i>A. citriodora</i>. The monoterpene (-)-carvone non-competitively inhibited the ACh-DRC (pD ́2 of 4.0±0.1) and the DRC of Ca2+ (pD ́2 of 3.86±0.19), suggesting that the Ca2+-influx blockade is the mechanism of its antispasmodic effect. Results suggest that the antispasmodic effect of <i>A. polystachya</i> and <i>A. gratissima</i> are mostly explained by the non-competitive blockade of Ca+2 influx. It could be associated to the presence of flavonoids, and in the tinctures to some spasmolytic components of the essential oil such as carvone. Facultad de Ciencias Exactas |
| description |
The antispasmodic effects of acqueous extracts (AE) and tinctures (T) of <i>Aloysia polystachya</i> (Griseb.) Moldenke and <i>Aloysia gratissima</i> (Gillies & Hook.) Tronc., Verbenaceae, were studied on rat isolated ileum and duodenum. These plants are used for gastrointestinal disorders and as eupeptic in South America. Both AE non-competitively inhibited the dose-response curves (DRC) of ACh and the DRC of Ca2+ in high-[K+]o, as well as the T. The T of <i>A. polystachya</i> and <i>A. gratissima</i> respectively inhibited the ACh-DRC at the IC50 of 3.15±0.57 and 6.46±2.28 mg leaves/mL. The Ca2+-antagonist activity of both T occurred with IC50 respectively similar to those of the ACh-DRC, and was potentiated by the depolarization produced by 10 mM TEA, a blocker of K+-channels. The spasmolytic effect of T does not involve DA release and binding to D2, since it was not reduced by 10 μM metoclopramide. Also, T induced dose-dependent relaxation on the tonic contracture produced by high-[K+]o and ACh. By TLC there were detected in the leaves the presence of carvone, and flavonoids such as quercetin and hesperidin. By HPLC there were not found vitexin nor isovitexin, identified in <i>A. citriodora</i>. The monoterpene (-)-carvone non-competitively inhibited the ACh-DRC (pD ́2 of 4.0±0.1) and the DRC of Ca2+ (pD ́2 of 3.86±0.19), suggesting that the Ca2+-influx blockade is the mechanism of its antispasmodic effect. Results suggest that the antispasmodic effect of <i>A. polystachya</i> and <i>A. gratissima</i> are mostly explained by the non-competitive blockade of Ca+2 influx. It could be associated to the presence of flavonoids, and in the tinctures to some spasmolytic components of the essential oil such as carvone. |
| publishDate |
2011 |
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2011-09 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/37521 |
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eng |
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eng |
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