Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus

Autores
Martinez, Valeria Paula; Padula, Paula Julieta
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Andes virus (ANDV) is responsible for the Hantavirus Pulmonary Syndrome cases in Argentina and neighboring countries, with moderate to high case-fatality rates. ANDV has some particular features, which make it unique among other members of the Hantavirus genus such as person-to-person transmission and causing a disease similar to Hantavirus Pulmonary Syndrome in the hamster as an animal model. The kinetics of replication in Vero E6 cells of an ANDV strain isolated in Argentina, called Andes/ARG, was studied. Cytopathic effect and the formation of clear plaques were observed and therefore Andes/ARG could be quantified by classic plaque assay. The Andes/ARG strain was found to be highly lethal in Syrian hamsters allowing experiments to demonstrate the protective potential of vaccines. A recombinant nucleocapsid protein of ANDV induced a long lasting antibody response and protective immunity against a homologous challenge, but to a lower extent against heterologous challenge by the Seoul virus.
Fil: Martinez, Valeria Paula. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Padula, Paula Julieta. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
HANTAVIRUS PULMONARY SYNDROME
MESOCRICETUS AURATUS
VACCINE CANDIDATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194666
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virusMartinez, Valeria PaulaPadula, Paula JulietaHANTAVIRUS PULMONARY SYNDROMEMESOCRICETUS AURATUSVACCINE CANDIDATEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Andes virus (ANDV) is responsible for the Hantavirus Pulmonary Syndrome cases in Argentina and neighboring countries, with moderate to high case-fatality rates. ANDV has some particular features, which make it unique among other members of the Hantavirus genus such as person-to-person transmission and causing a disease similar to Hantavirus Pulmonary Syndrome in the hamster as an animal model. The kinetics of replication in Vero E6 cells of an ANDV strain isolated in Argentina, called Andes/ARG, was studied. Cytopathic effect and the formation of clear plaques were observed and therefore Andes/ARG could be quantified by classic plaque assay. The Andes/ARG strain was found to be highly lethal in Syrian hamsters allowing experiments to demonstrate the protective potential of vaccines. A recombinant nucleocapsid protein of ANDV induced a long lasting antibody response and protective immunity against a homologous challenge, but to a lower extent against heterologous challenge by the Seoul virus.Fil: Martinez, Valeria Paula. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Padula, Paula Julieta. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley-liss, div John Wiley & Sons Inc.2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194666Martinez, Valeria Paula; Padula, Paula Julieta; Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 84; 1; 1-2012; 87-950146-6615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/Induction of protective immunity in a syrian hamster model against a cytopathogenic strain of andes virusinfo:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.22228info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:28Zoai:ri.conicet.gov.ar:11336/194666instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:28.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
title Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
spellingShingle Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
Martinez, Valeria Paula
HANTAVIRUS PULMONARY SYNDROME
MESOCRICETUS AURATUS
VACCINE CANDIDATE
title_short Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
title_full Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
title_fullStr Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
title_full_unstemmed Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
title_sort Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus
dc.creator.none.fl_str_mv Martinez, Valeria Paula
Padula, Paula Julieta
author Martinez, Valeria Paula
author_facet Martinez, Valeria Paula
Padula, Paula Julieta
author_role author
author2 Padula, Paula Julieta
author2_role author
dc.subject.none.fl_str_mv HANTAVIRUS PULMONARY SYNDROME
MESOCRICETUS AURATUS
VACCINE CANDIDATE
topic HANTAVIRUS PULMONARY SYNDROME
MESOCRICETUS AURATUS
VACCINE CANDIDATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Andes virus (ANDV) is responsible for the Hantavirus Pulmonary Syndrome cases in Argentina and neighboring countries, with moderate to high case-fatality rates. ANDV has some particular features, which make it unique among other members of the Hantavirus genus such as person-to-person transmission and causing a disease similar to Hantavirus Pulmonary Syndrome in the hamster as an animal model. The kinetics of replication in Vero E6 cells of an ANDV strain isolated in Argentina, called Andes/ARG, was studied. Cytopathic effect and the formation of clear plaques were observed and therefore Andes/ARG could be quantified by classic plaque assay. The Andes/ARG strain was found to be highly lethal in Syrian hamsters allowing experiments to demonstrate the protective potential of vaccines. A recombinant nucleocapsid protein of ANDV induced a long lasting antibody response and protective immunity against a homologous challenge, but to a lower extent against heterologous challenge by the Seoul virus.
Fil: Martinez, Valeria Paula. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina
Fil: Padula, Paula Julieta. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Andes virus (ANDV) is responsible for the Hantavirus Pulmonary Syndrome cases in Argentina and neighboring countries, with moderate to high case-fatality rates. ANDV has some particular features, which make it unique among other members of the Hantavirus genus such as person-to-person transmission and causing a disease similar to Hantavirus Pulmonary Syndrome in the hamster as an animal model. The kinetics of replication in Vero E6 cells of an ANDV strain isolated in Argentina, called Andes/ARG, was studied. Cytopathic effect and the formation of clear plaques were observed and therefore Andes/ARG could be quantified by classic plaque assay. The Andes/ARG strain was found to be highly lethal in Syrian hamsters allowing experiments to demonstrate the protective potential of vaccines. A recombinant nucleocapsid protein of ANDV induced a long lasting antibody response and protective immunity against a homologous challenge, but to a lower extent against heterologous challenge by the Seoul virus.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194666
Martinez, Valeria Paula; Padula, Paula Julieta; Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 84; 1; 1-2012; 87-95
0146-6615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194666
identifier_str_mv Martinez, Valeria Paula; Padula, Paula Julieta; Induction of protective immunity in a Syrian hamster model against a cytopathogenic strain of Andes virus; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 84; 1; 1-2012; 87-95
0146-6615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/Induction of protective immunity in a syrian hamster model against a cytopathogenic strain of andes virus
info:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.22228
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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