Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina

Autores
Gallo Calderon, Marina Beatriz; Romanutti, Carina; Wilda, Maximiliano; D'antuono, Alejandra Lorena; Keller, Leticia; Giacomodonato, Mónica Nancy; Mattion, Nora Marta; la Torre, Jose Leonardo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ninety-three rectal swab samples were taken, from dogs suspected of canine parvovirus (CPV) infectionand analyzed by PCR. A fragment of the VP2 gene, was amplified in 41 (44%) of them, resulting CPVpositive samples. Sequencing analysis of these PCR products showed that 37 samples (90.2%) belongedto the CPV2c type, whereas four samples (9.8%) were identified as CPV2a, which has not been found since2008.It was also found that 24 out of 37 CPV2c samples (65%), carried the mutation Thr440Ala, whereas thismutation was absent in the four CPV2a strains reported herein.Using phylogenetic analysis of the full length VP2 gene, which was amplified by PCR in six local samples,it was seen that CPV2a Argentine strains reported in this study, were genetically closer to a previouslocal CPV2a isolate (year 2003) and to a South African CPV2a strain, than to any of the recently reportedUruguayan CPV2a strains.The results obtained in this work, together with those reported previously in Uruguay strongly suggestthat, in spite of the geographical proximity, wild type CPV strains undergo different evolutive pathwaysin each country, resulting in the prevalence of different strains in related dog populations.Further extensive epidemiological studies are needed in order to improve the understanding of CPVevolution.
Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Keller, Leticia. Fundación de Estudios en Virología Animal; Argentina
Fil: Giacomodonato, Mónica Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Materia
Canine Parvovirus
Sequence Analysis
Virus Evolution
Vp2 Gene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4106
Acceder
id CONICETDig_2b6a97eaf51db6916ec7fea2890621f7
oai_identifier_str oai:ri.conicet.gov.ar:11336/4106
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Resurgence of canine parvovirus 2a strain in the domestic dog population from ArgentinaGallo Calderon, Marina BeatrizRomanutti, CarinaWilda, MaximilianoD'antuono, Alejandra LorenaKeller, LeticiaGiacomodonato, Mónica NancyMattion, Nora Martala Torre, Jose LeonardoCanine ParvovirusSequence AnalysisVirus EvolutionVp2 Genehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ninety-three rectal swab samples were taken, from dogs suspected of canine parvovirus (CPV) infectionand analyzed by PCR. A fragment of the VP2 gene, was amplified in 41 (44%) of them, resulting CPVpositive samples. Sequencing analysis of these PCR products showed that 37 samples (90.2%) belongedto the CPV2c type, whereas four samples (9.8%) were identified as CPV2a, which has not been found since2008.It was also found that 24 out of 37 CPV2c samples (65%), carried the mutation Thr440Ala, whereas thismutation was absent in the four CPV2a strains reported herein.Using phylogenetic analysis of the full length VP2 gene, which was amplified by PCR in six local samples,it was seen that CPV2a Argentine strains reported in this study, were genetically closer to a previouslocal CPV2a isolate (year 2003) and to a South African CPV2a strain, than to any of the recently reportedUruguayan CPV2a strains.The results obtained in this work, together with those reported previously in Uruguay strongly suggestthat, in spite of the geographical proximity, wild type CPV strains undergo different evolutive pathwaysin each country, resulting in the prevalence of different strains in related dog populations.Further extensive epidemiological studies are needed in order to improve the understanding of CPVevolution.Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Keller, Leticia. Fundación de Estudios en Virología Animal; ArgentinaFil: Giacomodonato, Mónica Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaElsevier2015-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4106Gallo Calderon, Marina Beatriz; Romanutti, Carina; Wilda, Maximiliano; D'antuono, Alejandra Lorena; Keller, Leticia; et al.; Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina; Elsevier; Journal of Virological Methods; 222; 24-6-2015; 145-1490166-0934enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093415002281info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2015.06.012info:eu-repo/semantics/altIdentifier/issn/0166-0934info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:25:49Zoai:ri.conicet.gov.ar:11336/4106instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:25:50.036CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
title Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
spellingShingle Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
Gallo Calderon, Marina Beatriz
Canine Parvovirus
Sequence Analysis
Virus Evolution
Vp2 Gene
title_short Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
title_full Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
title_fullStr Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
title_full_unstemmed Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
title_sort Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina
dc.creator.none.fl_str_mv Gallo Calderon, Marina Beatriz
Romanutti, Carina
Wilda, Maximiliano
D'antuono, Alejandra Lorena
Keller, Leticia
Giacomodonato, Mónica Nancy
Mattion, Nora Marta
la Torre, Jose Leonardo
author Gallo Calderon, Marina Beatriz
author_facet Gallo Calderon, Marina Beatriz
Romanutti, Carina
Wilda, Maximiliano
D'antuono, Alejandra Lorena
Keller, Leticia
Giacomodonato, Mónica Nancy
Mattion, Nora Marta
la Torre, Jose Leonardo
author_role author
author2 Romanutti, Carina
Wilda, Maximiliano
D'antuono, Alejandra Lorena
Keller, Leticia
Giacomodonato, Mónica Nancy
Mattion, Nora Marta
la Torre, Jose Leonardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Canine Parvovirus
Sequence Analysis
Virus Evolution
Vp2 Gene
topic Canine Parvovirus
Sequence Analysis
Virus Evolution
Vp2 Gene
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ninety-three rectal swab samples were taken, from dogs suspected of canine parvovirus (CPV) infectionand analyzed by PCR. A fragment of the VP2 gene, was amplified in 41 (44%) of them, resulting CPVpositive samples. Sequencing analysis of these PCR products showed that 37 samples (90.2%) belongedto the CPV2c type, whereas four samples (9.8%) were identified as CPV2a, which has not been found since2008.It was also found that 24 out of 37 CPV2c samples (65%), carried the mutation Thr440Ala, whereas thismutation was absent in the four CPV2a strains reported herein.Using phylogenetic analysis of the full length VP2 gene, which was amplified by PCR in six local samples,it was seen that CPV2a Argentine strains reported in this study, were genetically closer to a previouslocal CPV2a isolate (year 2003) and to a South African CPV2a strain, than to any of the recently reportedUruguayan CPV2a strains.The results obtained in this work, together with those reported previously in Uruguay strongly suggestthat, in spite of the geographical proximity, wild type CPV strains undergo different evolutive pathwaysin each country, resulting in the prevalence of different strains in related dog populations.Further extensive epidemiological studies are needed in order to improve the understanding of CPVevolution.
Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Romanutti, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Wilda, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Keller, Leticia. Fundación de Estudios en Virología Animal; Argentina
Fil: Giacomodonato, Mónica Nancy. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
description Ninety-three rectal swab samples were taken, from dogs suspected of canine parvovirus (CPV) infectionand analyzed by PCR. A fragment of the VP2 gene, was amplified in 41 (44%) of them, resulting CPVpositive samples. Sequencing analysis of these PCR products showed that 37 samples (90.2%) belongedto the CPV2c type, whereas four samples (9.8%) were identified as CPV2a, which has not been found since2008.It was also found that 24 out of 37 CPV2c samples (65%), carried the mutation Thr440Ala, whereas thismutation was absent in the four CPV2a strains reported herein.Using phylogenetic analysis of the full length VP2 gene, which was amplified by PCR in six local samples,it was seen that CPV2a Argentine strains reported in this study, were genetically closer to a previouslocal CPV2a isolate (year 2003) and to a South African CPV2a strain, than to any of the recently reportedUruguayan CPV2a strains.The results obtained in this work, together with those reported previously in Uruguay strongly suggestthat, in spite of the geographical proximity, wild type CPV strains undergo different evolutive pathwaysin each country, resulting in the prevalence of different strains in related dog populations.Further extensive epidemiological studies are needed in order to improve the understanding of CPVevolution.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4106
Gallo Calderon, Marina Beatriz; Romanutti, Carina; Wilda, Maximiliano; D'antuono, Alejandra Lorena; Keller, Leticia; et al.; Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina; Elsevier; Journal of Virological Methods; 222; 24-6-2015; 145-149
0166-0934
url http://hdl.handle.net/11336/4106
identifier_str_mv Gallo Calderon, Marina Beatriz; Romanutti, Carina; Wilda, Maximiliano; D'antuono, Alejandra Lorena; Keller, Leticia; et al.; Resurgence of canine parvovirus 2a strain in the domestic dog population from Argentina; Elsevier; Journal of Virological Methods; 222; 24-6-2015; 145-149
0166-0934
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093415002281
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2015.06.012
info:eu-repo/semantics/altIdentifier/issn/0166-0934
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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