Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata

Autores
Sabatini, Sebastian Eduardo; Brena, Beatríz M.; Pirez, Macarena; Rios, Maria del Carmen; Luquet, Carlos Marcelo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication.
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Brena, Beatríz M.. Universidad de la República; Uruguay
Fil: Pirez, Macarena. Universidad de la República; Uruguay
Fil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Materia
Elisa
Estuarine Crab
Hepatopancreas
Microcystin Depuration
Toxin Bioaccumulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54228
Acceder
id CONICETDig_2accb8012e7250ebb4e1e4db8809d219
oai_identifier_str oai:ri.conicet.gov.ar:11336/54228
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulataSabatini, Sebastian EduardoBrena, Beatríz M.Pirez, MacarenaRios, Maria del CarmenLuquet, Carlos MarceloElisaEstuarine CrabHepatopancreasMicrocystin DepurationToxin Bioaccumulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication.Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Brena, Beatríz M.. Universidad de la República; UruguayFil: Pirez, Macarena. Universidad de la República; UruguayFil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaAcademic Press Inc Elsevier Science2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54228Sabatini, Sebastian Eduardo; Brena, Beatríz M.; Pirez, Macarena; Rios, Maria del Carmen; Luquet, Carlos Marcelo; Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 120; 10-2015; 136-1410147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.05.041info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131500264Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:55Zoai:ri.conicet.gov.ar:11336/54228instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:55.98CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
spellingShingle Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
Sabatini, Sebastian Eduardo
Elisa
Estuarine Crab
Hepatopancreas
Microcystin Depuration
Toxin Bioaccumulation
title_short Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_full Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_fullStr Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_full_unstemmed Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
title_sort Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
dc.creator.none.fl_str_mv Sabatini, Sebastian Eduardo
Brena, Beatríz M.
Pirez, Macarena
Rios, Maria del Carmen
Luquet, Carlos Marcelo
author Sabatini, Sebastian Eduardo
author_facet Sabatini, Sebastian Eduardo
Brena, Beatríz M.
Pirez, Macarena
Rios, Maria del Carmen
Luquet, Carlos Marcelo
author_role author
author2 Brena, Beatríz M.
Pirez, Macarena
Rios, Maria del Carmen
Luquet, Carlos Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Elisa
Estuarine Crab
Hepatopancreas
Microcystin Depuration
Toxin Bioaccumulation
topic Elisa
Estuarine Crab
Hepatopancreas
Microcystin Depuration
Toxin Bioaccumulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication.
Fil: Sabatini, Sebastian Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Brena, Beatríz M.. Universidad de la República; Uruguay
Fil: Pirez, Macarena. Universidad de la República; Uruguay
Fil: Rios, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Luquet, Carlos Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
description We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54228
Sabatini, Sebastian Eduardo; Brena, Beatríz M.; Pirez, Macarena; Rios, Maria del Carmen; Luquet, Carlos Marcelo; Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 120; 10-2015; 136-141
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54228
identifier_str_mv Sabatini, Sebastian Eduardo; Brena, Beatríz M.; Pirez, Macarena; Rios, Maria del Carmen; Luquet, Carlos Marcelo; Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 120; 10-2015; 136-141
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2015.05.041
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S014765131500264X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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