Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury

Autores
Girouard, Marie Pier; Simas, Tristan; Hua, Luyang; Morquette, Barbara; Khazaei, Mohamad R.; Unsain, Nicolas; Johnstone, Aaron D.; Rambaldi, Isabel; Sanz, Ricardo L.; Di Raddo, Marie Eve; Gamage, Kanchana; Yong, Yu; Willis, Dianna; Verge, Valerie M. K.; Barker, Philip A.; Deppmann, Christopher; Fournier, Alyson
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and cytoskeletal disassembly of the distal segment. Collapsin response mediator protein 4 (CRMP4) is a cytosolic phospho-protein that regulates the actin and microtubule cytoskeleton. During development, CRMP4 promotes growth cone formation and dendrite development. Paradoxically, in the adult CNS, CRMP4 impedes axon regeneration. Here, we investigated the involvement of CRMP4 in peripheral nerve injury in male and female Crmp4/ mice following sciatic nerve injury. We find that sensory axon regeneration and Wallerian degeneration are impaired in Crmp4/ mice following sciatic nerve injury. In vitro analysis of dissociated dorsal root ganglion (DRG) neurons from Crmp4/ mice revealed that CRMP4 functions in the proximal axon segment to promote the regrowth of severed DRG neurons and in the distal axon segment where it facilitates Wallerian degeneration through calpain-dependent formation of harmful CRMP4 fragments. These findings reveal an interesting dual role for CRMP4 in proximal and distal axon segments of injured sensory neurons that coordinately facilitate PNS axon regeneration.
Fil: Girouard, Marie Pier. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Simas, Tristan. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Hua, Luyang. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Morquette, Barbara. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Khazaei, Mohamad R.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Unsain, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Johnstone, Aaron D.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Rambaldi, Isabel. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Sanz, Ricardo L.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Di Raddo, Marie Eve. McGill University; Canadá
Fil: Gamage, Kanchana. University of Virginia; Estados Unidos
Fil: Yong, Yu. University of Virginia; Estados Unidos
Fil: Willis, Dianna. Weill Cornell Medicine; Estados Unidos. Burke Neurological Institute; Estados Unidos
Fil: Verge, Valerie M. K.. University of Saskatchewan; Canadá
Fil: Barker, Philip A.. McGill University. Montreal Neurological Institute and Hospital; Canadá. University of British Columbia; Canadá
Fil: Deppmann, Christopher. University of Virginia; Estados Unidos
Fil: Fournier, Alyson. McGill University. Montreal Neurological Institute and Hospital; Canadá
Materia
CALPAIN
COLLAPSIN RESPONSE MEDIATOR PROTEIN
NEURONAL REGENERATION
SCIATIC NERVE INJURY
SENSORY NEURONS
WALLERIAN DEGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140272
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/140272
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve InjuryGirouard, Marie PierSimas, TristanHua, LuyangMorquette, BarbaraKhazaei, Mohamad R.Unsain, NicolasJohnstone, Aaron D.Rambaldi, IsabelSanz, Ricardo L.Di Raddo, Marie EveGamage, KanchanaYong, YuWillis, DiannaVerge, Valerie M. K.Barker, Philip A.Deppmann, ChristopherFournier, AlysonCALPAINCOLLAPSIN RESPONSE MEDIATOR PROTEINNEURONAL REGENERATIONSCIATIC NERVE INJURYSENSORY NEURONSWALLERIAN DEGENERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and cytoskeletal disassembly of the distal segment. Collapsin response mediator protein 4 (CRMP4) is a cytosolic phospho-protein that regulates the actin and microtubule cytoskeleton. During development, CRMP4 promotes growth cone formation and dendrite development. Paradoxically, in the adult CNS, CRMP4 impedes axon regeneration. Here, we investigated the involvement of CRMP4 in peripheral nerve injury in male and female Crmp4/ mice following sciatic nerve injury. We find that sensory axon regeneration and Wallerian degeneration are impaired in Crmp4/ mice following sciatic nerve injury. In vitro analysis of dissociated dorsal root ganglion (DRG) neurons from Crmp4/ mice revealed that CRMP4 functions in the proximal axon segment to promote the regrowth of severed DRG neurons and in the distal axon segment where it facilitates Wallerian degeneration through calpain-dependent formation of harmful CRMP4 fragments. These findings reveal an interesting dual role for CRMP4 in proximal and distal axon segments of injured sensory neurons that coordinately facilitate PNS axon regeneration.Fil: Girouard, Marie Pier. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Simas, Tristan. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Hua, Luyang. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Morquette, Barbara. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Khazaei, Mohamad R.. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Unsain, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Johnstone, Aaron D.. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Rambaldi, Isabel. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Sanz, Ricardo L.. McGill University. Montreal Neurological Institute and Hospital; CanadáFil: Di Raddo, Marie Eve. McGill University; CanadáFil: Gamage, Kanchana. University of Virginia; Estados UnidosFil: Yong, Yu. University of Virginia; Estados UnidosFil: Willis, Dianna. Weill Cornell Medicine; Estados Unidos. Burke Neurological Institute; Estados UnidosFil: Verge, Valerie M. K.. University of Saskatchewan; CanadáFil: Barker, Philip A.. McGill University. Montreal Neurological Institute and Hospital; Canadá. University of British Columbia; CanadáFil: Deppmann, Christopher. University of Virginia; Estados UnidosFil: Fournier, Alyson. McGill University. Montreal Neurological Institute and Hospital; CanadáSociety for Neuroscience2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140272Girouard, Marie Pier; Simas, Tristan; Hua, Luyang; Morquette, Barbara; Khazaei, Mohamad R.; et al.; Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury; Society for Neuroscience; eNeuro; 7; 2; 3-2020; 1-172373-2822CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://eneuro.org/lookup/doi/10.1523/ENEURO.0479-19.2020info:eu-repo/semantics/altIdentifier/doi/10.1523/ENEURO.0479-19.2020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:12Zoai:ri.conicet.gov.ar:11336/140272instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:12.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
title Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
spellingShingle Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
Girouard, Marie Pier
CALPAIN
COLLAPSIN RESPONSE MEDIATOR PROTEIN
NEURONAL REGENERATION
SCIATIC NERVE INJURY
SENSORY NEURONS
WALLERIAN DEGENERATION
title_short Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
title_full Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
title_fullStr Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
title_full_unstemmed Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
title_sort Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury
dc.creator.none.fl_str_mv Girouard, Marie Pier
Simas, Tristan
Hua, Luyang
Morquette, Barbara
Khazaei, Mohamad R.
Unsain, Nicolas
Johnstone, Aaron D.
Rambaldi, Isabel
Sanz, Ricardo L.
Di Raddo, Marie Eve
Gamage, Kanchana
Yong, Yu
Willis, Dianna
Verge, Valerie M. K.
Barker, Philip A.
Deppmann, Christopher
Fournier, Alyson
author Girouard, Marie Pier
author_facet Girouard, Marie Pier
Simas, Tristan
Hua, Luyang
Morquette, Barbara
Khazaei, Mohamad R.
Unsain, Nicolas
Johnstone, Aaron D.
Rambaldi, Isabel
Sanz, Ricardo L.
Di Raddo, Marie Eve
Gamage, Kanchana
Yong, Yu
Willis, Dianna
Verge, Valerie M. K.
Barker, Philip A.
Deppmann, Christopher
Fournier, Alyson
author_role author
author2 Simas, Tristan
Hua, Luyang
Morquette, Barbara
Khazaei, Mohamad R.
Unsain, Nicolas
Johnstone, Aaron D.
Rambaldi, Isabel
Sanz, Ricardo L.
Di Raddo, Marie Eve
Gamage, Kanchana
Yong, Yu
Willis, Dianna
Verge, Valerie M. K.
Barker, Philip A.
Deppmann, Christopher
Fournier, Alyson
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CALPAIN
COLLAPSIN RESPONSE MEDIATOR PROTEIN
NEURONAL REGENERATION
SCIATIC NERVE INJURY
SENSORY NEURONS
WALLERIAN DEGENERATION
topic CALPAIN
COLLAPSIN RESPONSE MEDIATOR PROTEIN
NEURONAL REGENERATION
SCIATIC NERVE INJURY
SENSORY NEURONS
WALLERIAN DEGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and cytoskeletal disassembly of the distal segment. Collapsin response mediator protein 4 (CRMP4) is a cytosolic phospho-protein that regulates the actin and microtubule cytoskeleton. During development, CRMP4 promotes growth cone formation and dendrite development. Paradoxically, in the adult CNS, CRMP4 impedes axon regeneration. Here, we investigated the involvement of CRMP4 in peripheral nerve injury in male and female Crmp4/ mice following sciatic nerve injury. We find that sensory axon regeneration and Wallerian degeneration are impaired in Crmp4/ mice following sciatic nerve injury. In vitro analysis of dissociated dorsal root ganglion (DRG) neurons from Crmp4/ mice revealed that CRMP4 functions in the proximal axon segment to promote the regrowth of severed DRG neurons and in the distal axon segment where it facilitates Wallerian degeneration through calpain-dependent formation of harmful CRMP4 fragments. These findings reveal an interesting dual role for CRMP4 in proximal and distal axon segments of injured sensory neurons that coordinately facilitate PNS axon regeneration.
Fil: Girouard, Marie Pier. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Simas, Tristan. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Hua, Luyang. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Morquette, Barbara. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Khazaei, Mohamad R.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Unsain, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Johnstone, Aaron D.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Rambaldi, Isabel. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Sanz, Ricardo L.. McGill University. Montreal Neurological Institute and Hospital; Canadá
Fil: Di Raddo, Marie Eve. McGill University; Canadá
Fil: Gamage, Kanchana. University of Virginia; Estados Unidos
Fil: Yong, Yu. University of Virginia; Estados Unidos
Fil: Willis, Dianna. Weill Cornell Medicine; Estados Unidos. Burke Neurological Institute; Estados Unidos
Fil: Verge, Valerie M. K.. University of Saskatchewan; Canadá
Fil: Barker, Philip A.. McGill University. Montreal Neurological Institute and Hospital; Canadá. University of British Columbia; Canadá
Fil: Deppmann, Christopher. University of Virginia; Estados Unidos
Fil: Fournier, Alyson. McGill University. Montreal Neurological Institute and Hospital; Canadá
description In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and cytoskeletal disassembly of the distal segment. Collapsin response mediator protein 4 (CRMP4) is a cytosolic phospho-protein that regulates the actin and microtubule cytoskeleton. During development, CRMP4 promotes growth cone formation and dendrite development. Paradoxically, in the adult CNS, CRMP4 impedes axon regeneration. Here, we investigated the involvement of CRMP4 in peripheral nerve injury in male and female Crmp4/ mice following sciatic nerve injury. We find that sensory axon regeneration and Wallerian degeneration are impaired in Crmp4/ mice following sciatic nerve injury. In vitro analysis of dissociated dorsal root ganglion (DRG) neurons from Crmp4/ mice revealed that CRMP4 functions in the proximal axon segment to promote the regrowth of severed DRG neurons and in the distal axon segment where it facilitates Wallerian degeneration through calpain-dependent formation of harmful CRMP4 fragments. These findings reveal an interesting dual role for CRMP4 in proximal and distal axon segments of injured sensory neurons that coordinately facilitate PNS axon regeneration.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140272
Girouard, Marie Pier; Simas, Tristan; Hua, Luyang; Morquette, Barbara; Khazaei, Mohamad R.; et al.; Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury; Society for Neuroscience; eNeuro; 7; 2; 3-2020; 1-17
2373-2822
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140272
identifier_str_mv Girouard, Marie Pier; Simas, Tristan; Hua, Luyang; Morquette, Barbara; Khazaei, Mohamad R.; et al.; Collapsin Response Mediator Protein 4 (CRMP4) Facilitates Wallerian Degeneration and Axon Regeneration following Sciatic Nerve Injury; Society for Neuroscience; eNeuro; 7; 2; 3-2020; 1-17
2373-2822
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://eneuro.org/lookup/doi/10.1523/ENEURO.0479-19.2020
info:eu-repo/semantics/altIdentifier/doi/10.1523/ENEURO.0479-19.2020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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