All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration

Autores
Piñero, Gonzalo Miguel; Vence, Marianela; Aranda, Marcos Luis; Cercato, Magalí Cecilia; Soto, Paula Andrea; Usach, Vanina; Setton Avruj, Patricia C.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Working on a Wallerian degeneration model in rats, our group has shown the beneficial effects of systemic bone marrow mononuclear cell transplant on morphological and functional parameters, as well as the prevention of neuropathic pain. The current work thus seeks to evaluate the effect of systemic bone marrow cell transplant in a mouse model of sciatic nerve crush and aims to dig deeper into the mechanisms involved in bone marrow cell therapy. Adult C57BL/6J mice were subjected to 8s sciatic nerve crush and intravenously transplanted with bone marrow cells. Cells were tracked using a fluorescent probe, and the evolution of the degeneration–regeneration process was evaluated through axonal and myelin marker immunodetection at different survival times. Gene and protein expression of the main cytokines involved in the inflammatory phase and lesion-associated macrophage phenotypes were also analyzed. Initial findings corroborated the beneficial effect of bone marrow cells on the regenerative process and proved their efficiency in reducing the expression of some proinflammatory cytokines and increasing that of anti-inflammatory interleukin 10 (IL-10). In addition, transplanted animals showed a decrease in inducible nitric oxide synthase (iNOS)+ macrophages, an increment in CD206+ cells, and an anticipated rise in Arg-1+ macrophages. Taken together, our results endorse bone marrow cell therapy as an alternative approach to accelerate nerve recovery and postulate bone marrow cells as potential immunomodulators. Summary Statement: Bone marrow cell transplant has proven to be an effective therapeutic approach to treat peripheral nervous system injuries as it not only promoted regeneration and remyelination of the injured nerve but also had a potent effect on neuropathic pain.
Fil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Vence, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Aranda, Marcos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Cercato, Magalí Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Setton Avruj, Patricia C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
BONE MARROW CELLS
IMMUNOMODULATION
REMYELINATION
WALLERIAN DEGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227612
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227612
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve RegenerationPiñero, Gonzalo MiguelVence, MarianelaAranda, Marcos LuisCercato, Magalí CeciliaSoto, Paula AndreaUsach, VaninaSetton Avruj, Patricia C.BONE MARROW CELLSIMMUNOMODULATIONREMYELINATIONWALLERIAN DEGENERATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Working on a Wallerian degeneration model in rats, our group has shown the beneficial effects of systemic bone marrow mononuclear cell transplant on morphological and functional parameters, as well as the prevention of neuropathic pain. The current work thus seeks to evaluate the effect of systemic bone marrow cell transplant in a mouse model of sciatic nerve crush and aims to dig deeper into the mechanisms involved in bone marrow cell therapy. Adult C57BL/6J mice were subjected to 8s sciatic nerve crush and intravenously transplanted with bone marrow cells. Cells were tracked using a fluorescent probe, and the evolution of the degeneration–regeneration process was evaluated through axonal and myelin marker immunodetection at different survival times. Gene and protein expression of the main cytokines involved in the inflammatory phase and lesion-associated macrophage phenotypes were also analyzed. Initial findings corroborated the beneficial effect of bone marrow cells on the regenerative process and proved their efficiency in reducing the expression of some proinflammatory cytokines and increasing that of anti-inflammatory interleukin 10 (IL-10). In addition, transplanted animals showed a decrease in inducible nitric oxide synthase (iNOS)+ macrophages, an increment in CD206+ cells, and an anticipated rise in Arg-1+ macrophages. Taken together, our results endorse bone marrow cell therapy as an alternative approach to accelerate nerve recovery and postulate bone marrow cells as potential immunomodulators. Summary Statement: Bone marrow cell transplant has proven to be an effective therapeutic approach to treat peripheral nervous system injuries as it not only promoted regeneration and remyelination of the injured nerve but also had a potent effect on neuropathic pain.Fil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Vence, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Aranda, Marcos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Cercato, Magalí Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Setton Avruj, Patricia C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaSAGE Publications2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227612Piñero, Gonzalo Miguel; Vence, Marianela; Aranda, Marcos Luis; Cercato, Magalí Cecilia; Soto, Paula Andrea; et al.; All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration; SAGE Publications; ASN Neuro; 15; 9-2023; 1-191759-09141759-0914CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/17590914231167281info:eu-repo/semantics/altIdentifier/doi/10.1177/17590914231167281info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:17:47Zoai:ri.conicet.gov.ar:11336/227612instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:17:47.42CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
title All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
spellingShingle All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
Piñero, Gonzalo Miguel
BONE MARROW CELLS
IMMUNOMODULATION
REMYELINATION
WALLERIAN DEGENERATION
title_short All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
title_full All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
title_fullStr All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
title_full_unstemmed All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
title_sort All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration
dc.creator.none.fl_str_mv Piñero, Gonzalo Miguel
Vence, Marianela
Aranda, Marcos Luis
Cercato, Magalí Cecilia
Soto, Paula Andrea
Usach, Vanina
Setton Avruj, Patricia C.
author Piñero, Gonzalo Miguel
author_facet Piñero, Gonzalo Miguel
Vence, Marianela
Aranda, Marcos Luis
Cercato, Magalí Cecilia
Soto, Paula Andrea
Usach, Vanina
Setton Avruj, Patricia C.
author_role author
author2 Vence, Marianela
Aranda, Marcos Luis
Cercato, Magalí Cecilia
Soto, Paula Andrea
Usach, Vanina
Setton Avruj, Patricia C.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BONE MARROW CELLS
IMMUNOMODULATION
REMYELINATION
WALLERIAN DEGENERATION
topic BONE MARROW CELLS
IMMUNOMODULATION
REMYELINATION
WALLERIAN DEGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Working on a Wallerian degeneration model in rats, our group has shown the beneficial effects of systemic bone marrow mononuclear cell transplant on morphological and functional parameters, as well as the prevention of neuropathic pain. The current work thus seeks to evaluate the effect of systemic bone marrow cell transplant in a mouse model of sciatic nerve crush and aims to dig deeper into the mechanisms involved in bone marrow cell therapy. Adult C57BL/6J mice were subjected to 8s sciatic nerve crush and intravenously transplanted with bone marrow cells. Cells were tracked using a fluorescent probe, and the evolution of the degeneration–regeneration process was evaluated through axonal and myelin marker immunodetection at different survival times. Gene and protein expression of the main cytokines involved in the inflammatory phase and lesion-associated macrophage phenotypes were also analyzed. Initial findings corroborated the beneficial effect of bone marrow cells on the regenerative process and proved their efficiency in reducing the expression of some proinflammatory cytokines and increasing that of anti-inflammatory interleukin 10 (IL-10). In addition, transplanted animals showed a decrease in inducible nitric oxide synthase (iNOS)+ macrophages, an increment in CD206+ cells, and an anticipated rise in Arg-1+ macrophages. Taken together, our results endorse bone marrow cell therapy as an alternative approach to accelerate nerve recovery and postulate bone marrow cells as potential immunomodulators. Summary Statement: Bone marrow cell transplant has proven to be an effective therapeutic approach to treat peripheral nervous system injuries as it not only promoted regeneration and remyelination of the injured nerve but also had a potent effect on neuropathic pain.
Fil: Piñero, Gonzalo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Vence, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Aranda, Marcos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Cercato, Magalí Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Usach, Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Setton Avruj, Patricia C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Working on a Wallerian degeneration model in rats, our group has shown the beneficial effects of systemic bone marrow mononuclear cell transplant on morphological and functional parameters, as well as the prevention of neuropathic pain. The current work thus seeks to evaluate the effect of systemic bone marrow cell transplant in a mouse model of sciatic nerve crush and aims to dig deeper into the mechanisms involved in bone marrow cell therapy. Adult C57BL/6J mice were subjected to 8s sciatic nerve crush and intravenously transplanted with bone marrow cells. Cells were tracked using a fluorescent probe, and the evolution of the degeneration–regeneration process was evaluated through axonal and myelin marker immunodetection at different survival times. Gene and protein expression of the main cytokines involved in the inflammatory phase and lesion-associated macrophage phenotypes were also analyzed. Initial findings corroborated the beneficial effect of bone marrow cells on the regenerative process and proved their efficiency in reducing the expression of some proinflammatory cytokines and increasing that of anti-inflammatory interleukin 10 (IL-10). In addition, transplanted animals showed a decrease in inducible nitric oxide synthase (iNOS)+ macrophages, an increment in CD206+ cells, and an anticipated rise in Arg-1+ macrophages. Taken together, our results endorse bone marrow cell therapy as an alternative approach to accelerate nerve recovery and postulate bone marrow cells as potential immunomodulators. Summary Statement: Bone marrow cell transplant has proven to be an effective therapeutic approach to treat peripheral nervous system injuries as it not only promoted regeneration and remyelination of the injured nerve but also had a potent effect on neuropathic pain.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227612
Piñero, Gonzalo Miguel; Vence, Marianela; Aranda, Marcos Luis; Cercato, Magalí Cecilia; Soto, Paula Andrea; et al.; All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration; SAGE Publications; ASN Neuro; 15; 9-2023; 1-19
1759-0914
1759-0914
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227612
identifier_str_mv Piñero, Gonzalo Miguel; Vence, Marianela; Aranda, Marcos Luis; Cercato, Magalí Cecilia; Soto, Paula Andrea; et al.; All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration; SAGE Publications; ASN Neuro; 15; 9-2023; 1-19
1759-0914
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/17590914231167281
info:eu-repo/semantics/altIdentifier/doi/10.1177/17590914231167281
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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