Regulation of vascular homeostasis by plant derived estrogens
- Autores
- Sandoval, Marisa Julia; Cepeda, Sabrina Belén; Massheimer, Virginia Laura
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology - Materia
-
PHYTOESTROGENS
GENISTEIN
VASCULAR CALCIFICATION
ANGIOGENESIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/184403
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Regulation of vascular homeostasis by plant derived estrogensSandoval, Marisa JuliaCepeda, Sabrina BelénMassheimer, Virginia LauraPHYTOESTROGENSGENISTEINVASCULAR CALCIFICATIONANGIOGENESIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”Buenos AiresArgentinaInternational Society of EndocrinologyInternational Society of Endocrinology2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184403Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:49Zoai:ri.conicet.gov.ar:11336/184403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:50.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Regulation of vascular homeostasis by plant derived estrogens |
title |
Regulation of vascular homeostasis by plant derived estrogens |
spellingShingle |
Regulation of vascular homeostasis by plant derived estrogens Sandoval, Marisa Julia PHYTOESTROGENS GENISTEIN VASCULAR CALCIFICATION ANGIOGENESIS |
title_short |
Regulation of vascular homeostasis by plant derived estrogens |
title_full |
Regulation of vascular homeostasis by plant derived estrogens |
title_fullStr |
Regulation of vascular homeostasis by plant derived estrogens |
title_full_unstemmed |
Regulation of vascular homeostasis by plant derived estrogens |
title_sort |
Regulation of vascular homeostasis by plant derived estrogens |
dc.creator.none.fl_str_mv |
Sandoval, Marisa Julia Cepeda, Sabrina Belén Massheimer, Virginia Laura |
author |
Sandoval, Marisa Julia |
author_facet |
Sandoval, Marisa Julia Cepeda, Sabrina Belén Massheimer, Virginia Laura |
author_role |
author |
author2 |
Cepeda, Sabrina Belén Massheimer, Virginia Laura |
author2_role |
author author |
dc.subject.none.fl_str_mv |
PHYTOESTROGENS GENISTEIN VASCULAR CALCIFICATION ANGIOGENESIS |
topic |
PHYTOESTROGENS GENISTEIN VASCULAR CALCIFICATION ANGIOGENESIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis. Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology” Buenos Aires Argentina International Society of Endocrinology |
description |
Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
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conferenceObject |
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http://hdl.handle.net/11336/184403 Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/184403 |
identifier_str_mv |
Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/ |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
International Society of Endocrinology |
publisher.none.fl_str_mv |
International Society of Endocrinology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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