Regulation of vascular homeostasis by plant derived estrogens

Autores
Sandoval, Marisa Julia; Cepeda, Sabrina Belén; Massheimer, Virginia Laura
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology
Materia
PHYTOESTROGENS
GENISTEIN
VASCULAR CALCIFICATION
ANGIOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184403
Acceder
id CONICETDig_277efdc78ced45c2a32f525632eedd17
oai_identifier_str oai:ri.conicet.gov.ar:11336/184403
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of vascular homeostasis by plant derived estrogensSandoval, Marisa JuliaCepeda, Sabrina BelénMassheimer, Virginia LauraPHYTOESTROGENSGENISTEINVASCULAR CALCIFICATIONANGIOGENESIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”Buenos AiresArgentinaInternational Society of EndocrinologyInternational Society of Endocrinology2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184403Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:16Zoai:ri.conicet.gov.ar:11336/184403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:16.897CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of vascular homeostasis by plant derived estrogens
title Regulation of vascular homeostasis by plant derived estrogens
spellingShingle Regulation of vascular homeostasis by plant derived estrogens
Sandoval, Marisa Julia
PHYTOESTROGENS
GENISTEIN
VASCULAR CALCIFICATION
ANGIOGENESIS
title_short Regulation of vascular homeostasis by plant derived estrogens
title_full Regulation of vascular homeostasis by plant derived estrogens
title_fullStr Regulation of vascular homeostasis by plant derived estrogens
title_full_unstemmed Regulation of vascular homeostasis by plant derived estrogens
title_sort Regulation of vascular homeostasis by plant derived estrogens
dc.creator.none.fl_str_mv Sandoval, Marisa Julia
Cepeda, Sabrina Belén
Massheimer, Virginia Laura
author Sandoval, Marisa Julia
author_facet Sandoval, Marisa Julia
Cepeda, Sabrina Belén
Massheimer, Virginia Laura
author_role author
author2 Cepeda, Sabrina Belén
Massheimer, Virginia Laura
author2_role author
author
dc.subject.none.fl_str_mv PHYTOESTROGENS
GENISTEIN
VASCULAR CALCIFICATION
ANGIOGENESIS
topic PHYTOESTROGENS
GENISTEIN
VASCULAR CALCIFICATION
ANGIOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”
Buenos Aires
Argentina
International Society of Endocrinology
description Phytoestrogens (PE) such as genistein (Gen) are considered a natural therapy to counteract the risk of cardiovascular diseases in postmenopausal women. Atherosclerosis is a chronic inflammatory process that, in the later step of plaque generation could conduct to vascular calcification (VCa). Revascularization, mainly aided by angiogenesis, represents a survival mechanism for ischemic tissue. We have previously reported that Gen prevents cellular events involved in the early stages of plaque formation. In this work we study the effect of Gen on VCa and angiogenesis. Aortic rings (AR); endothelial cells (EC) and vascular smooth muscle cells (VSMC) isolated from aortic explants of female Wistar rats were employed. Cells cultures (VSMC; EC) or AR were in vitro exposed to 1-100 nM of Gen. When the effect of Gen on VCa was assayed, intact aortas were cultured in procalcifying medium (10 mM β-glycerophosphate; 4 mM CaCl2) for 14 days. A significant reduction in calcified areas, revealed by AgNO3 staining, was detected after 10 nM Gen treatment. These results were confirmed by quantification of aortic tissue calcium content (552±43 vs 235±18 ug Ca/mg prot, C vs Gen, p<0.001). Since VCa depends on VSMC transdifferentiation into osteoblast, VSMC cells were cultured for 21 days in procalcifying medium. Extracellular calcium content and alkaline phosphatase (ALP) activity were measured as osteoblastic differentiation markers. Gen induced 0,5 and 1 fold reduction in ALP activity (p<0.05) and calcium deposition (p<0.05) respectively, suggesting an inhibitory action on VSMC transdifferentiation into bone like cells. In view that angiogenesis involves EC proliferation, migration and VEGF synthesis; we studied the role of Gen on these events. Gen (4-96 h) stimulated EC proliferation at all concentrations tested (22-39% above C, p <0.05, MTT assay), with higher effect at short time interval treatment. After 72 h of treatment, the PE enhanced EC migration (20%- 66% above C, 10-100 nM, p<0.01; wound healing assay). Indeed, Gen increased VEGF synthesis (24.6-68.9 % above C, 1-10 nM Gen; ELISA assay). We confirmed that EC were able to proliferate after VEGF exposure. In order to evaluate new capillaries tube formation, AR were seeded on a collagen matrix and exposed to Gen for 15 d. Gen induced a 10-fold increase in tube formation around AR. In conclusion, the PE exhibits a potential beneficial effect on vascular homeostasis by reducing VCa and promoting angiogenesis.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184403
Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184403
identifier_str_mv Regulation of vascular homeostasis by plant derived estrogens; 19th International Congress of Endocrinology under the theme of “Global Partnership in Facing the Current Challenges in Endocrinology”; Buenos Aires; Argentina; 2021; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.isendo.org/event/ice-virtual-congress-2021/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Society of Endocrinology
publisher.none.fl_str_mv International Society of Endocrinology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613020522643456
score 13.069144

Publicaciones similares