Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling
- Autores
- Cutini, Pablo Hernan; Rauschemberger, María Belén; Massheimer, Virginia Laura
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The genesis of the atheromatous lesion involves the interaction of monocytes and platelets with the endothelium. Finally, vascular calcification takes place through vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The impact of bisphosphonates (BP) on cardiovascular diseases remains unknown. The aim of this study was to investigate the role of the BP alendronate (ALN) in cellular and molecular processes involved in vascular disease. Murine cultures cells were used: a) endothelial cells (EC); b) VSMC; c) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glicerolfostato 5 mM; CaCl2 and 4 mM). The effect of ALN on platelet adhesion (PAd) and aggregation (PAg) was investigated. Under basal conditions ALN partially reduced PAd (20–35%; ALN 1–10 μM; p<0.01). The stimulus induced by LPS was partially reduced in the presence of the BP (22–33%; ALN 1–10 μM; p<0.02). We found that ALN markedly inhibited PAg compared with control (56%; ALN 10 μM; p<0.05). Both antiplatelet effects evoked by ALN were reversed in the presence of L-NAME, an inhibitor of nitric oxide synthase. Monocyte adhesion to EC depends on the expression of adhesion molecules. ALN treatment significantly prevents the enhancement of ICAM-1 and VCAM-1 mRNA levels induced by LPS. On VSMC-OB, ALN markedly reduce the expression of RUNX2 and TNAP, showed a significant diminution in FAL activity (9–29%; ALN 1–10 μM; p<0.02), as well as in extracellular calcium deposition (341 ± 33 vs 225 ± 24 μg/mg prot.; control vs ALN 5 μM; p<0.05). In summary, the results suggest that ALN is an active drug at vascular level with potential beneficial effects on processes that compromise the vascular architecture.
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Rauschemberger, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO)
Ciudad Autónoma de Buenos Aires
Argentina
Asociación Argentina de Osteología y Metabolismo Mineral
Sociedad Argentina de Osteoporois - Materia
-
ALENDRONATE
VASCULAR
CALCIFICATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/235027
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Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodelingCutini, Pablo HernanRauschemberger, María BelénMassheimer, Virginia LauraALENDRONATEVASCULARCALCIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The genesis of the atheromatous lesion involves the interaction of monocytes and platelets with the endothelium. Finally, vascular calcification takes place through vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The impact of bisphosphonates (BP) on cardiovascular diseases remains unknown. The aim of this study was to investigate the role of the BP alendronate (ALN) in cellular and molecular processes involved in vascular disease. Murine cultures cells were used: a) endothelial cells (EC); b) VSMC; c) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glicerolfostato 5 mM; CaCl2 and 4 mM). The effect of ALN on platelet adhesion (PAd) and aggregation (PAg) was investigated. Under basal conditions ALN partially reduced PAd (20–35%; ALN 1–10 μM; p<0.01). The stimulus induced by LPS was partially reduced in the presence of the BP (22–33%; ALN 1–10 μM; p<0.02). We found that ALN markedly inhibited PAg compared with control (56%; ALN 10 μM; p<0.05). Both antiplatelet effects evoked by ALN were reversed in the presence of L-NAME, an inhibitor of nitric oxide synthase. Monocyte adhesion to EC depends on the expression of adhesion molecules. ALN treatment significantly prevents the enhancement of ICAM-1 and VCAM-1 mRNA levels induced by LPS. On VSMC-OB, ALN markedly reduce the expression of RUNX2 and TNAP, showed a significant diminution in FAL activity (9–29%; ALN 1–10 μM; p<0.02), as well as in extracellular calcium deposition (341 ± 33 vs 225 ± 24 μg/mg prot.; control vs ALN 5 μM; p<0.05). In summary, the results suggest that ALN is an active drug at vascular level with potential beneficial effects on processes that compromise the vascular architecture.Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Rauschemberger, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO)Ciudad Autónoma de Buenos AiresArgentinaAsociación Argentina de Osteología y Metabolismo MineralSociedad Argentina de OsteoporoisElsevierRoland, Baron2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235027Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling; 1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO); Ciudad Autónoma de Buenos Aires; Argentina; 2016; 302-3021873-27638756-3282CONICET DigitalCONICETenghttps://www.gador.com.ar/congresos/aaomm-sao-2016-xxxiii-reunion-anual-aaomm-xii-congreso-argentino-osteoporosis-cao-2016/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2017.03.021info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S875632821730090XNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:05Zoai:ri.conicet.gov.ar:11336/235027instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:05.388CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
title |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
spellingShingle |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling Cutini, Pablo Hernan ALENDRONATE VASCULAR CALCIFICATION |
title_short |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
title_full |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
title_fullStr |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
title_full_unstemmed |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
title_sort |
Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling |
dc.creator.none.fl_str_mv |
Cutini, Pablo Hernan Rauschemberger, María Belén Massheimer, Virginia Laura |
author |
Cutini, Pablo Hernan |
author_facet |
Cutini, Pablo Hernan Rauschemberger, María Belén Massheimer, Virginia Laura |
author_role |
author |
author2 |
Rauschemberger, María Belén Massheimer, Virginia Laura |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Roland, Baron |
dc.subject.none.fl_str_mv |
ALENDRONATE VASCULAR CALCIFICATION |
topic |
ALENDRONATE VASCULAR CALCIFICATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The genesis of the atheromatous lesion involves the interaction of monocytes and platelets with the endothelium. Finally, vascular calcification takes place through vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The impact of bisphosphonates (BP) on cardiovascular diseases remains unknown. The aim of this study was to investigate the role of the BP alendronate (ALN) in cellular and molecular processes involved in vascular disease. Murine cultures cells were used: a) endothelial cells (EC); b) VSMC; c) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glicerolfostato 5 mM; CaCl2 and 4 mM). The effect of ALN on platelet adhesion (PAd) and aggregation (PAg) was investigated. Under basal conditions ALN partially reduced PAd (20–35%; ALN 1–10 μM; p<0.01). The stimulus induced by LPS was partially reduced in the presence of the BP (22–33%; ALN 1–10 μM; p<0.02). We found that ALN markedly inhibited PAg compared with control (56%; ALN 10 μM; p<0.05). Both antiplatelet effects evoked by ALN were reversed in the presence of L-NAME, an inhibitor of nitric oxide synthase. Monocyte adhesion to EC depends on the expression of adhesion molecules. ALN treatment significantly prevents the enhancement of ICAM-1 and VCAM-1 mRNA levels induced by LPS. On VSMC-OB, ALN markedly reduce the expression of RUNX2 and TNAP, showed a significant diminution in FAL activity (9–29%; ALN 1–10 μM; p<0.02), as well as in extracellular calcium deposition (341 ± 33 vs 225 ± 24 μg/mg prot.; control vs ALN 5 μM; p<0.05). In summary, the results suggest that ALN is an active drug at vascular level with potential beneficial effects on processes that compromise the vascular architecture. Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Rauschemberger, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina 1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO) Ciudad Autónoma de Buenos Aires Argentina Asociación Argentina de Osteología y Metabolismo Mineral Sociedad Argentina de Osteoporois |
description |
The genesis of the atheromatous lesion involves the interaction of monocytes and platelets with the endothelium. Finally, vascular calcification takes place through vascular smooth muscle cells (VSMC) transdifferentiation to bone lineage. The impact of bisphosphonates (BP) on cardiovascular diseases remains unknown. The aim of this study was to investigate the role of the BP alendronate (ALN) in cellular and molecular processes involved in vascular disease. Murine cultures cells were used: a) endothelial cells (EC); b) VSMC; c) VSMC induced to osteoblastic transdifferentiation (VSMC-OB) in osteogenic medium (β-glicerolfostato 5 mM; CaCl2 and 4 mM). The effect of ALN on platelet adhesion (PAd) and aggregation (PAg) was investigated. Under basal conditions ALN partially reduced PAd (20–35%; ALN 1–10 μM; p<0.01). The stimulus induced by LPS was partially reduced in the presence of the BP (22–33%; ALN 1–10 μM; p<0.02). We found that ALN markedly inhibited PAg compared with control (56%; ALN 10 μM; p<0.05). Both antiplatelet effects evoked by ALN were reversed in the presence of L-NAME, an inhibitor of nitric oxide synthase. Monocyte adhesion to EC depends on the expression of adhesion molecules. ALN treatment significantly prevents the enhancement of ICAM-1 and VCAM-1 mRNA levels induced by LPS. On VSMC-OB, ALN markedly reduce the expression of RUNX2 and TNAP, showed a significant diminution in FAL activity (9–29%; ALN 1–10 μM; p<0.02), as well as in extracellular calcium deposition (341 ± 33 vs 225 ± 24 μg/mg prot.; control vs ALN 5 μM; p<0.05). In summary, the results suggest that ALN is an active drug at vascular level with potential beneficial effects on processes that compromise the vascular architecture. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
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conferenceObject |
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http://hdl.handle.net/11336/235027 Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling; 1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO); Ciudad Autónoma de Buenos Aires; Argentina; 2016; 302-302 1873-2763 8756-3282 CONICET Digital CONICET |
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http://hdl.handle.net/11336/235027 |
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Beneficial role of alendronate on cellular and molecular processes involved in calcification/vascular remodeling; 1º Congreso Argentino de Osteología - XXXIII Reunión Anual de la AAOMM - XII Congreso Argentino de Osteoporosis (CAO); Ciudad Autónoma de Buenos Aires; Argentina; 2016; 302-302 1873-2763 8756-3282 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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https://www.gador.com.ar/congresos/aaomm-sao-2016-xxxiii-reunion-anual-aaomm-xii-congreso-argentino-osteoporosis-cao-2016/ info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2017.03.021 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S875632821730090X |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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