Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)

Autores
Barroetaveña, Carolina; Pildain, María Belén; Salgado Salomón, María Eugenia; Eberhart, J. L.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ponderosa pine (Pinus ponderosa Dougl. ex P. & C. Laws.), an ectomycorrhiza (EM) dependent species, has been widely introduced in Patagonia, Argentina. This study used morphotyping, restriction analysis, and sequencing of EM root tips from ponderosa pine seedlings in two nurseries to assess the complete EM fungus (EMF) richness, to confirm doubtful identities of commonly reported morphotypes, and to evaluate the efficiency of morphotyping compared with molecular analysis. This interdisciplinary approach together with the fact that is the first study in which Patagonian nurseries EMF are genetically evaluated contributes to the general knowledge of this important group of fungi. Sequencing revealed the presence of 11 taxa. Basidiomycetes included Thelephoraceae (Tomentella sp.), Atheliaceae (Amphinema byssoides (Pers.) J. Erikss.), Hydnangiaceae (Laccaria sp.), Rhizopogonaceae (Rhizopogon roseolus (Corda) Th. Fr.), and Cortinariaceae (Hebeloma mesophaeum (Pers.) Quel.). Ascomycetes included Pezizaceae (Wilcoxina mikolae (Chin S. Yang & HE. Wilcox) Chin S. Yang & Korf and Wilcoxina sp.) and Tuberaceae (Tuber sp.). Morphotyping proved to be useful for certain EMF species (R. roseolus, H. mesophaeum, A. byssoides, and to a lesser extent Tuber sp.) in which some morphological features are conspicuous and unique. Our detection of W. mikolae and Wilcoxina sp. are new records for ponderosa pine in Patagonia. All of the EM taxa identified are common to pine plantations and nurseries around the world, and no indigenous EM associated with native Nothofagus spp. were found.
Fil: Barroetaveña, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Salgado Salomón, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel. Departamento de Ingeniería Forestal; Argentina
Fil: Eberhart, J. L.. State University of Oregon; Estados Unidos
Materia
AMPHINEMA BYSSOIDES
RHIZOPOGON ROSEOLUS
PCR-ITS-RFLP
SEQUENCING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189936
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)Barroetaveña, CarolinaPildain, María BelénSalgado Salomón, María EugeniaEberhart, J. L.AMPHINEMA BYSSOIDESRHIZOPOGON ROSEOLUSPCR-ITS-RFLPSEQUENCINGhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Ponderosa pine (Pinus ponderosa Dougl. ex P. & C. Laws.), an ectomycorrhiza (EM) dependent species, has been widely introduced in Patagonia, Argentina. This study used morphotyping, restriction analysis, and sequencing of EM root tips from ponderosa pine seedlings in two nurseries to assess the complete EM fungus (EMF) richness, to confirm doubtful identities of commonly reported morphotypes, and to evaluate the efficiency of morphotyping compared with molecular analysis. This interdisciplinary approach together with the fact that is the first study in which Patagonian nurseries EMF are genetically evaluated contributes to the general knowledge of this important group of fungi. Sequencing revealed the presence of 11 taxa. Basidiomycetes included Thelephoraceae (Tomentella sp.), Atheliaceae (Amphinema byssoides (Pers.) J. Erikss.), Hydnangiaceae (Laccaria sp.), Rhizopogonaceae (Rhizopogon roseolus (Corda) Th. Fr.), and Cortinariaceae (Hebeloma mesophaeum (Pers.) Quel.). Ascomycetes included Pezizaceae (Wilcoxina mikolae (Chin S. Yang & HE. Wilcox) Chin S. Yang & Korf and Wilcoxina sp.) and Tuberaceae (Tuber sp.). Morphotyping proved to be useful for certain EMF species (R. roseolus, H. mesophaeum, A. byssoides, and to a lesser extent Tuber sp.) in which some morphological features are conspicuous and unique. Our detection of W. mikolae and Wilcoxina sp. are new records for ponderosa pine in Patagonia. All of the EM taxa identified are common to pine plantations and nurseries around the world, and no indigenous EM associated with native Nothofagus spp. were found.Fil: Barroetaveña, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; ArgentinaFil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; ArgentinaFil: Salgado Salomón, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel. Departamento de Ingeniería Forestal; ArgentinaFil: Eberhart, J. L.. State University of Oregon; Estados UnidosNational Research Council Canada-NRC Research Press2010-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189936Barroetaveña, Carolina; Pildain, María Belén; Salgado Salomón, María Eugenia; Eberhart, J. L.; Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina); National Research Council Canada-NRC Research Press; Canadian Journal Of Forest Research; 40; 10; 9-2010; 1940-19500045-5067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/X10-135info:eu-repo/semantics/altIdentifier/doi/10.1139/X10-135info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:33Zoai:ri.conicet.gov.ar:11336/189936instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:34.279CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
title Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
spellingShingle Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
Barroetaveña, Carolina
AMPHINEMA BYSSOIDES
RHIZOPOGON ROSEOLUS
PCR-ITS-RFLP
SEQUENCING
title_short Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
title_full Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
title_fullStr Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
title_full_unstemmed Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
title_sort Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina)
dc.creator.none.fl_str_mv Barroetaveña, Carolina
Pildain, María Belén
Salgado Salomón, María Eugenia
Eberhart, J. L.
author Barroetaveña, Carolina
author_facet Barroetaveña, Carolina
Pildain, María Belén
Salgado Salomón, María Eugenia
Eberhart, J. L.
author_role author
author2 Pildain, María Belén
Salgado Salomón, María Eugenia
Eberhart, J. L.
author2_role author
author
author
dc.subject.none.fl_str_mv AMPHINEMA BYSSOIDES
RHIZOPOGON ROSEOLUS
PCR-ITS-RFLP
SEQUENCING
topic AMPHINEMA BYSSOIDES
RHIZOPOGON ROSEOLUS
PCR-ITS-RFLP
SEQUENCING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Ponderosa pine (Pinus ponderosa Dougl. ex P. & C. Laws.), an ectomycorrhiza (EM) dependent species, has been widely introduced in Patagonia, Argentina. This study used morphotyping, restriction analysis, and sequencing of EM root tips from ponderosa pine seedlings in two nurseries to assess the complete EM fungus (EMF) richness, to confirm doubtful identities of commonly reported morphotypes, and to evaluate the efficiency of morphotyping compared with molecular analysis. This interdisciplinary approach together with the fact that is the first study in which Patagonian nurseries EMF are genetically evaluated contributes to the general knowledge of this important group of fungi. Sequencing revealed the presence of 11 taxa. Basidiomycetes included Thelephoraceae (Tomentella sp.), Atheliaceae (Amphinema byssoides (Pers.) J. Erikss.), Hydnangiaceae (Laccaria sp.), Rhizopogonaceae (Rhizopogon roseolus (Corda) Th. Fr.), and Cortinariaceae (Hebeloma mesophaeum (Pers.) Quel.). Ascomycetes included Pezizaceae (Wilcoxina mikolae (Chin S. Yang & HE. Wilcox) Chin S. Yang & Korf and Wilcoxina sp.) and Tuberaceae (Tuber sp.). Morphotyping proved to be useful for certain EMF species (R. roseolus, H. mesophaeum, A. byssoides, and to a lesser extent Tuber sp.) in which some morphological features are conspicuous and unique. Our detection of W. mikolae and Wilcoxina sp. are new records for ponderosa pine in Patagonia. All of the EM taxa identified are common to pine plantations and nurseries around the world, and no indigenous EM associated with native Nothofagus spp. were found.
Fil: Barroetaveña, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Pildain, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina
Fil: Salgado Salomón, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ingeniería - Sede Esquel. Departamento de Ingeniería Forestal; Argentina
Fil: Eberhart, J. L.. State University of Oregon; Estados Unidos
description Ponderosa pine (Pinus ponderosa Dougl. ex P. & C. Laws.), an ectomycorrhiza (EM) dependent species, has been widely introduced in Patagonia, Argentina. This study used morphotyping, restriction analysis, and sequencing of EM root tips from ponderosa pine seedlings in two nurseries to assess the complete EM fungus (EMF) richness, to confirm doubtful identities of commonly reported morphotypes, and to evaluate the efficiency of morphotyping compared with molecular analysis. This interdisciplinary approach together with the fact that is the first study in which Patagonian nurseries EMF are genetically evaluated contributes to the general knowledge of this important group of fungi. Sequencing revealed the presence of 11 taxa. Basidiomycetes included Thelephoraceae (Tomentella sp.), Atheliaceae (Amphinema byssoides (Pers.) J. Erikss.), Hydnangiaceae (Laccaria sp.), Rhizopogonaceae (Rhizopogon roseolus (Corda) Th. Fr.), and Cortinariaceae (Hebeloma mesophaeum (Pers.) Quel.). Ascomycetes included Pezizaceae (Wilcoxina mikolae (Chin S. Yang & HE. Wilcox) Chin S. Yang & Korf and Wilcoxina sp.) and Tuberaceae (Tuber sp.). Morphotyping proved to be useful for certain EMF species (R. roseolus, H. mesophaeum, A. byssoides, and to a lesser extent Tuber sp.) in which some morphological features are conspicuous and unique. Our detection of W. mikolae and Wilcoxina sp. are new records for ponderosa pine in Patagonia. All of the EM taxa identified are common to pine plantations and nurseries around the world, and no indigenous EM associated with native Nothofagus spp. were found.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189936
Barroetaveña, Carolina; Pildain, María Belén; Salgado Salomón, María Eugenia; Eberhart, J. L.; Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina); National Research Council Canada-NRC Research Press; Canadian Journal Of Forest Research; 40; 10; 9-2010; 1940-1950
0045-5067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189936
identifier_str_mv Barroetaveña, Carolina; Pildain, María Belén; Salgado Salomón, María Eugenia; Eberhart, J. L.; Molecular identification of ectomycorrhizas associated with ponderosa pine seedlings in Patagonian nurseries (Argentina); National Research Council Canada-NRC Research Press; Canadian Journal Of Forest Research; 40; 10; 9-2010; 1940-1950
0045-5067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://cdnsciencepub.com/doi/10.1139/X10-135
info:eu-repo/semantics/altIdentifier/doi/10.1139/X10-135
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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