Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
- Autores
- Guzmán, Fabiana; Giolitti, Fabian; Fernandez, Franco Daniel; Nome Docampo, Claudia; Lenardon, Sergio Luis; Conci, Luis Rogelio
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.
Instituto de Patología Vegetal
Fil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina - Fuente
- European journal of plant pathology 138 (4) : 679–683. (April 2014)
- Materia
-
Phytoplasmas
Fitoplasmas
Helianthus Annuus
Argentina
Girasol
RFLP-PCR
Secuenciación
Sunflower
Sequencing
16SrDNA
X-disease - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/3763
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Identification and molecular characterization of a phytoplasma associated with sunflower in ArgentinaGuzmán, FabianaGiolitti, FabianFernandez, Franco DanielNome Docampo, ClaudiaLenardon, Sergio LuisConci, Luis RogelioPhytoplasmasFitoplasmasHelianthus AnnuusArgentinaGirasolRFLP-PCRSecuenciaciónSunflowerSequencing16SrDNAX-diseaseSunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.Instituto de Patología VegetalFil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; ArgentinaSpringer2018-11-01T17:27:20Z2018-11-01T17:27:20Z2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3763https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas0929-18731573-8469 (Online)https://doi.org/10.1007/s10658-013-0352-yEuropean journal of plant pathology 138 (4) : 679–683. (April 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:21Zoai:localhost:20.500.12123/3763instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:21.572INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
title |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
spellingShingle |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina Guzmán, Fabiana Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease |
title_short |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
title_full |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
title_fullStr |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
title_full_unstemmed |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
title_sort |
Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
dc.creator.none.fl_str_mv |
Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio |
author |
Guzmán, Fabiana |
author_facet |
Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio |
author_role |
author |
author2 |
Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease |
topic |
Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease |
dc.description.none.fl_txt_mv |
Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower. Instituto de Patología Vegetal Fil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina |
description |
Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04 2018-11-01T17:27:20Z 2018-11-01T17:27:20Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/3763 https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas 0929-1873 1573-8469 (Online) https://doi.org/10.1007/s10658-013-0352-y |
url |
http://hdl.handle.net/20.500.12123/3763 https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas https://doi.org/10.1007/s10658-013-0352-y |
identifier_str_mv |
0929-1873 1573-8469 (Online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
European journal of plant pathology 138 (4) : 679–683. (April 2014) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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13.234792 |