Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina

Autores
Guzmán, Fabiana; Giolitti, Fabian; Fernandez, Franco Daniel; Nome Docampo, Claudia; Lenardon, Sergio Luis; Conci, Luis Rogelio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.
Instituto de Patología Vegetal
Fil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina
Fuente
European journal of plant pathology 138 (4) : 679–683. (April 2014)
Materia
Phytoplasmas
Fitoplasmas
Helianthus Annuus
Argentina
Girasol
RFLP-PCR
Secuenciación
Sunflower
Sequencing
16SrDNA
X-disease
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3763
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3763
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network_name_str INTA Digital (INTA)
spelling Identification and molecular characterization of a phytoplasma associated with sunflower in ArgentinaGuzmán, FabianaGiolitti, FabianFernandez, Franco DanielNome Docampo, ClaudiaLenardon, Sergio LuisConci, Luis RogelioPhytoplasmasFitoplasmasHelianthus AnnuusArgentinaGirasolRFLP-PCRSecuenciaciónSunflowerSequencing16SrDNAX-diseaseSunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.Instituto de Patología VegetalFil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; ArgentinaSpringer2018-11-01T17:27:20Z2018-11-01T17:27:20Z2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3763https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas0929-18731573-8469 (Online)https://doi.org/10.1007/s10658-013-0352-yEuropean journal of plant pathology 138 (4) : 679–683. (April 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:21Zoai:localhost:20.500.12123/3763instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:21.572INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
title Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
spellingShingle Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
Guzmán, Fabiana
Phytoplasmas
Fitoplasmas
Helianthus Annuus
Argentina
Girasol
RFLP-PCR
Secuenciación
Sunflower
Sequencing
16SrDNA
X-disease
title_short Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
title_full Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
title_fullStr Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
title_full_unstemmed Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
title_sort Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
dc.creator.none.fl_str_mv Guzmán, Fabiana
Giolitti, Fabian
Fernandez, Franco Daniel
Nome Docampo, Claudia
Lenardon, Sergio Luis
Conci, Luis Rogelio
author Guzmán, Fabiana
author_facet Guzmán, Fabiana
Giolitti, Fabian
Fernandez, Franco Daniel
Nome Docampo, Claudia
Lenardon, Sergio Luis
Conci, Luis Rogelio
author_role author
author2 Giolitti, Fabian
Fernandez, Franco Daniel
Nome Docampo, Claudia
Lenardon, Sergio Luis
Conci, Luis Rogelio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Phytoplasmas
Fitoplasmas
Helianthus Annuus
Argentina
Girasol
RFLP-PCR
Secuenciación
Sunflower
Sequencing
16SrDNA
X-disease
topic Phytoplasmas
Fitoplasmas
Helianthus Annuus
Argentina
Girasol
RFLP-PCR
Secuenciación
Sunflower
Sequencing
16SrDNA
X-disease
dc.description.none.fl_txt_mv Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.
Instituto de Patología Vegetal
Fil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina
description Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
2018-11-01T17:27:20Z
2018-11-01T17:27:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3763
https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas
0929-1873
1573-8469 (Online)
https://doi.org/10.1007/s10658-013-0352-y
url http://hdl.handle.net/20.500.12123/3763
https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas
https://doi.org/10.1007/s10658-013-0352-y
identifier_str_mv 0929-1873
1573-8469 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European journal of plant pathology 138 (4) : 679–683. (April 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.234792

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