Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)

Autores
Sanabria, Rodrigo Eduardo Fabrizio; Moré, Gastón Andrés; Romero, Jorge Roberto
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paramphistomosis has shown an increased spread over the last years in Argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. Even when morphological features were reported, molecular characterization appears as a complementary and reliable tool. Samples of Paramphistomum leydeni collected since 2002 from different origin, definitive hosts, natural or experimental infections and preservation method were subjected to PCR-RFLP for ITS2+ fragment, and compared to Fasciola hepatica and Notocotilidae cercarie. Frozen and ethanol fixed samples amplified fragments of 500 bp for P. leydeni and Notocotilidae samples, while F. hepatica eggs produced a 540 bp amplicon. Restriction fragments obtained from endonucleases HinfI, HhaI, BsuRI, TaqI, and TruI were identical for all P. leydeni samples, supporting the morphological classification previously performed. Four selected amplicons were sequenced and reported at GenBank, given a consensus sequence ITS-2+ of 441 bp. This first report of molecular characterization for P. leydeni, improves the current knowledge of the genus and establishes precedents for further specimen classifications.
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Materia
Paramphistomum leydeni
PCR-RFLP
Sequencing
ITS-2+
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95057
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)Sanabria, Rodrigo Eduardo FabrizioMoré, Gastón AndrésRomero, Jorge RobertoParamphistomum leydeniPCR-RFLPSequencingITS-2+https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Paramphistomosis has shown an increased spread over the last years in Argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. Even when morphological features were reported, molecular characterization appears as a complementary and reliable tool. Samples of Paramphistomum leydeni collected since 2002 from different origin, definitive hosts, natural or experimental infections and preservation method were subjected to PCR-RFLP for ITS2+ fragment, and compared to Fasciola hepatica and Notocotilidae cercarie. Frozen and ethanol fixed samples amplified fragments of 500 bp for P. leydeni and Notocotilidae samples, while F. hepatica eggs produced a 540 bp amplicon. Restriction fragments obtained from endonucleases HinfI, HhaI, BsuRI, TaqI, and TruI were identical for all P. leydeni samples, supporting the morphological classification previously performed. Four selected amplicons were sequenced and reported at GenBank, given a consensus sequence ITS-2+ of 441 bp. This first report of molecular characterization for P. leydeni, improves the current knowledge of the genus and establishes precedents for further specimen classifications.Fil: Sanabria, Rodrigo Eduardo Fabrizio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaElsevier Science2011-04-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95057Sanabria, Rodrigo Eduardo Fabrizio; Moré, Gastón Andrés; Romero, Jorge Roberto; Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae); Elsevier Science; Veterinary Parasitology; 177; 1-2; 19-4-2011; 182-1850304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401710006606info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2010.11.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:54Zoai:ri.conicet.gov.ar:11336/95057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:54.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
title Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
spellingShingle Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
Sanabria, Rodrigo Eduardo Fabrizio
Paramphistomum leydeni
PCR-RFLP
Sequencing
ITS-2+
title_short Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
title_full Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
title_fullStr Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
title_full_unstemmed Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
title_sort Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
dc.creator.none.fl_str_mv Sanabria, Rodrigo Eduardo Fabrizio
Moré, Gastón Andrés
Romero, Jorge Roberto
author Sanabria, Rodrigo Eduardo Fabrizio
author_facet Sanabria, Rodrigo Eduardo Fabrizio
Moré, Gastón Andrés
Romero, Jorge Roberto
author_role author
author2 Moré, Gastón Andrés
Romero, Jorge Roberto
author2_role author
author
dc.subject.none.fl_str_mv Paramphistomum leydeni
PCR-RFLP
Sequencing
ITS-2+
topic Paramphistomum leydeni
PCR-RFLP
Sequencing
ITS-2+
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Paramphistomosis has shown an increased spread over the last years in Argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. Even when morphological features were reported, molecular characterization appears as a complementary and reliable tool. Samples of Paramphistomum leydeni collected since 2002 from different origin, definitive hosts, natural or experimental infections and preservation method were subjected to PCR-RFLP for ITS2+ fragment, and compared to Fasciola hepatica and Notocotilidae cercarie. Frozen and ethanol fixed samples amplified fragments of 500 bp for P. leydeni and Notocotilidae samples, while F. hepatica eggs produced a 540 bp amplicon. Restriction fragments obtained from endonucleases HinfI, HhaI, BsuRI, TaqI, and TruI were identical for all P. leydeni samples, supporting the morphological classification previously performed. Four selected amplicons were sequenced and reported at GenBank, given a consensus sequence ITS-2+ of 441 bp. This first report of molecular characterization for P. leydeni, improves the current knowledge of the genus and establishes precedents for further specimen classifications.
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Moré, Gastón Andrés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clínica. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
description Paramphistomosis has shown an increased spread over the last years in Argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. Even when morphological features were reported, molecular characterization appears as a complementary and reliable tool. Samples of Paramphistomum leydeni collected since 2002 from different origin, definitive hosts, natural or experimental infections and preservation method were subjected to PCR-RFLP for ITS2+ fragment, and compared to Fasciola hepatica and Notocotilidae cercarie. Frozen and ethanol fixed samples amplified fragments of 500 bp for P. leydeni and Notocotilidae samples, while F. hepatica eggs produced a 540 bp amplicon. Restriction fragments obtained from endonucleases HinfI, HhaI, BsuRI, TaqI, and TruI were identical for all P. leydeni samples, supporting the morphological classification previously performed. Four selected amplicons were sequenced and reported at GenBank, given a consensus sequence ITS-2+ of 441 bp. This first report of molecular characterization for P. leydeni, improves the current knowledge of the genus and establishes precedents for further specimen classifications.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95057
Sanabria, Rodrigo Eduardo Fabrizio; Moré, Gastón Andrés; Romero, Jorge Roberto; Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae); Elsevier Science; Veterinary Parasitology; 177; 1-2; 19-4-2011; 182-185
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95057
identifier_str_mv Sanabria, Rodrigo Eduardo Fabrizio; Moré, Gastón Andrés; Romero, Jorge Roberto; Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae); Elsevier Science; Veterinary Parasitology; 177; 1-2; 19-4-2011; 182-185
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304401710006606
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2010.11.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083557568544768
score 13.22299

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