Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance

Autores
Fuentes Suguiyama, Vanessa; Paredes Rodriguez, Jae Diana; Nicomedio dos Santos, Tatiane Cristina; Lira, Bruno Silvestre; de Haro, Luis Alejandro; Naldi Silva, João Paulo; Leite Borba, Eduardo; Purgatto, Eduardo; Alves da Silva, Emerson; Bellora, Nicolás; Carrari, Fernando Oscar; da Cruz Centeno, Danilo; Bermudez Salazar, Luisa Fernanda; Rossi, Magdalena; de Setta, Nathalia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.
Fil: Fuentes Suguiyama, Vanessa. Universidad Federal do Abc; Brasil
Fil: Paredes Rodriguez, Jae Diana. Universidad Federal do Abc; Brasil
Fil: Nicomedio dos Santos, Tatiane Cristina. Universidad Federal do Abc; Brasil
Fil: Lira, Bruno Silvestre. Universidade de Sao Paulo; Brasil
Fil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Naldi Silva, João Paulo. Universidad Federal do Abc; Brasil
Fil: Leite Borba, Eduardo. Universidade Federal de Minas Gerais; Brasil
Fil: Purgatto, Eduardo. Universidade de Sao Paulo; Brasil
Fil: Alves da Silva, Emerson. No especifíca;
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrari, Fernando Oscar. Universidad de Buenos Aires; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética; Argentina
Fil: da Cruz Centeno, Danilo. Universidad Federal do Abc; Brasil
Fil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
Fil: de Setta, Nathalia. Universidad Federal do Abc; Brasil
Materia
MONOCOTS
PLANT METABOLISM
SRNAS
TRANSCRIPTOME
TRANSPOSABLE ELEMENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216364
Acceder
id CONICETDig_25f21c8946fe3bb237fba862c9b1ddc7
oai_identifier_str oai:ri.conicet.gov.ar:11336/216364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit toleranceFuentes Suguiyama, VanessaParedes Rodriguez, Jae DianaNicomedio dos Santos, Tatiane CristinaLira, Bruno Silvestrede Haro, Luis AlejandroNaldi Silva, João PauloLeite Borba, EduardoPurgatto, EduardoAlves da Silva, EmersonBellora, NicolásCarrari, Fernando Oscarda Cruz Centeno, DaniloBermudez Salazar, Luisa FernandaRossi, Magdalenade Setta, NathaliaMONOCOTSPLANT METABOLISMSRNASTRANSCRIPTOMETRANSPOSABLE ELEMENTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.Fil: Fuentes Suguiyama, Vanessa. Universidad Federal do Abc; BrasilFil: Paredes Rodriguez, Jae Diana. Universidad Federal do Abc; BrasilFil: Nicomedio dos Santos, Tatiane Cristina. Universidad Federal do Abc; BrasilFil: Lira, Bruno Silvestre. Universidade de Sao Paulo; BrasilFil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Naldi Silva, João Paulo. Universidad Federal do Abc; BrasilFil: Leite Borba, Eduardo. Universidade Federal de Minas Gerais; BrasilFil: Purgatto, Eduardo. Universidade de Sao Paulo; BrasilFil: Alves da Silva, Emerson. No especifíca;Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carrari, Fernando Oscar. Universidad de Buenos Aires; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética; ArgentinaFil: da Cruz Centeno, Danilo. Universidad Federal do Abc; BrasilFil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rossi, Magdalena. Universidade de Sao Paulo; BrasilFil: de Setta, Nathalia. Universidad Federal do Abc; BrasilSpringer2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216364Fuentes Suguiyama, Vanessa; Paredes Rodriguez, Jae Diana; Nicomedio dos Santos, Tatiane Cristina; Lira, Bruno Silvestre; de Haro, Luis Alejandro; et al.; Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance; Springer; Plant Molecular Biology; 109; 6; 5-2022; 761-7800167-4412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11103-022-01273-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:50Zoai:ri.conicet.gov.ar:11336/216364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:51.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
title Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
spellingShingle Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
Fuentes Suguiyama, Vanessa
MONOCOTS
PLANT METABOLISM
SRNAS
TRANSCRIPTOME
TRANSPOSABLE ELEMENTS
title_short Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
title_full Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
title_fullStr Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
title_full_unstemmed Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
title_sort Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
dc.creator.none.fl_str_mv Fuentes Suguiyama, Vanessa
Paredes Rodriguez, Jae Diana
Nicomedio dos Santos, Tatiane Cristina
Lira, Bruno Silvestre
de Haro, Luis Alejandro
Naldi Silva, João Paulo
Leite Borba, Eduardo
Purgatto, Eduardo
Alves da Silva, Emerson
Bellora, Nicolás
Carrari, Fernando Oscar
da Cruz Centeno, Danilo
Bermudez Salazar, Luisa Fernanda
Rossi, Magdalena
de Setta, Nathalia
author Fuentes Suguiyama, Vanessa
author_facet Fuentes Suguiyama, Vanessa
Paredes Rodriguez, Jae Diana
Nicomedio dos Santos, Tatiane Cristina
Lira, Bruno Silvestre
de Haro, Luis Alejandro
Naldi Silva, João Paulo
Leite Borba, Eduardo
Purgatto, Eduardo
Alves da Silva, Emerson
Bellora, Nicolás
Carrari, Fernando Oscar
da Cruz Centeno, Danilo
Bermudez Salazar, Luisa Fernanda
Rossi, Magdalena
de Setta, Nathalia
author_role author
author2 Paredes Rodriguez, Jae Diana
Nicomedio dos Santos, Tatiane Cristina
Lira, Bruno Silvestre
de Haro, Luis Alejandro
Naldi Silva, João Paulo
Leite Borba, Eduardo
Purgatto, Eduardo
Alves da Silva, Emerson
Bellora, Nicolás
Carrari, Fernando Oscar
da Cruz Centeno, Danilo
Bermudez Salazar, Luisa Fernanda
Rossi, Magdalena
de Setta, Nathalia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MONOCOTS
PLANT METABOLISM
SRNAS
TRANSCRIPTOME
TRANSPOSABLE ELEMENTS
topic MONOCOTS
PLANT METABOLISM
SRNAS
TRANSCRIPTOME
TRANSPOSABLE ELEMENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.
Fil: Fuentes Suguiyama, Vanessa. Universidad Federal do Abc; Brasil
Fil: Paredes Rodriguez, Jae Diana. Universidad Federal do Abc; Brasil
Fil: Nicomedio dos Santos, Tatiane Cristina. Universidad Federal do Abc; Brasil
Fil: Lira, Bruno Silvestre. Universidade de Sao Paulo; Brasil
Fil: de Haro, Luis Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Naldi Silva, João Paulo. Universidad Federal do Abc; Brasil
Fil: Leite Borba, Eduardo. Universidade Federal de Minas Gerais; Brasil
Fil: Purgatto, Eduardo. Universidade de Sao Paulo; Brasil
Fil: Alves da Silva, Emerson. No especifíca;
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrari, Fernando Oscar. Universidad de Buenos Aires; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Genética; Argentina
Fil: da Cruz Centeno, Danilo. Universidad Federal do Abc; Brasil
Fil: Bermudez Salazar, Luisa Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
Fil: de Setta, Nathalia. Universidad Federal do Abc; Brasil
description Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.
publishDate 2022
dc.date.none.fl_str_mv 2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216364
Fuentes Suguiyama, Vanessa; Paredes Rodriguez, Jae Diana; Nicomedio dos Santos, Tatiane Cristina; Lira, Bruno Silvestre; de Haro, Luis Alejandro; et al.; Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance; Springer; Plant Molecular Biology; 109; 6; 5-2022; 761-780
0167-4412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216364
identifier_str_mv Fuentes Suguiyama, Vanessa; Paredes Rodriguez, Jae Diana; Nicomedio dos Santos, Tatiane Cristina; Lira, Bruno Silvestre; de Haro, Luis Alejandro; et al.; Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance; Springer; Plant Molecular Biology; 109; 6; 5-2022; 761-780
0167-4412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11103-022-01273-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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