Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion

Autores
Vaschetto, Luis Maria Benjamin
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited. Nonetheless, TEs have been initially proposed as positive control elements acting in synergy with the host. Nowadays, it is well known that TE movement into host genome comprises an important evolutionary mechanism capable of increasing the adaptive fitness. As insights into TE functioning are increasing day to day, the manipulation of transposition has raised an interesting possibility of setting the host functions, although the lack of appropriate genome engineering tools has unpaved it. Fortunately, the emergence of genome editing technologies based on programmable nucleases, and especially the arrival of a multipurpose RNA-guided Cas9 endonuclease system, has made it possible to reconsider this challenge. For such purpose, a particular type of transposons referred to as miniature inverted-repeat transposable elements (MITEs) has shown a series of interesting characteristics for designing functional drivers. Here, recent insights into MITE elements and versatile RNA-guided CRISPR/Cas9 genome engineering system are given to understand how to deploy the potential of TEs for control of the host transcriptional activity.
Fil: Vaschetto, Luis Maria Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; Argentina
Materia
CRISPR/CAS9
GENOME EDITING
MINIATURE INVERTED TRANSPOSABLE ELEMENTS
TE-BASED DRIVES
TRANSCRIPTIONAL CONTROL
TRANSPOSABLE ELEMENT AMPLIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51397
Acceder
id CONICETDig_5ffa49306c73220a725b12fdd0f572b8
oai_identifier_str oai:ri.conicet.gov.ar:11336/51397
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertionVaschetto, Luis Maria BenjaminCRISPR/CAS9GENOME EDITINGMINIATURE INVERTED TRANSPOSABLE ELEMENTSTE-BASED DRIVESTRANSCRIPTIONAL CONTROLTRANSPOSABLE ELEMENT AMPLIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited. Nonetheless, TEs have been initially proposed as positive control elements acting in synergy with the host. Nowadays, it is well known that TE movement into host genome comprises an important evolutionary mechanism capable of increasing the adaptive fitness. As insights into TE functioning are increasing day to day, the manipulation of transposition has raised an interesting possibility of setting the host functions, although the lack of appropriate genome engineering tools has unpaved it. Fortunately, the emergence of genome editing technologies based on programmable nucleases, and especially the arrival of a multipurpose RNA-guided Cas9 endonuclease system, has made it possible to reconsider this challenge. For such purpose, a particular type of transposons referred to as miniature inverted-repeat transposable elements (MITEs) has shown a series of interesting characteristics for designing functional drivers. Here, recent insights into MITE elements and versatile RNA-guided CRISPR/Cas9 genome engineering system are given to understand how to deploy the potential of TEs for control of the host transcriptional activity.Fil: Vaschetto, Luis Maria Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; ArgentinaSpringer2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51397Vaschetto, Luis Maria Benjamin; Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion; Springer; Current Genetics; 64; 2; 4-2018; 405-4120172-8083CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00294-017-0765-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00294-017-0765-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:13Zoai:ri.conicet.gov.ar:11336/51397instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:14.278CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
title Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
spellingShingle Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
Vaschetto, Luis Maria Benjamin
CRISPR/CAS9
GENOME EDITING
MINIATURE INVERTED TRANSPOSABLE ELEMENTS
TE-BASED DRIVES
TRANSCRIPTIONAL CONTROL
TRANSPOSABLE ELEMENT AMPLIFICATION
title_short Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
title_full Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
title_fullStr Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
title_full_unstemmed Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
title_sort Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion
dc.creator.none.fl_str_mv Vaschetto, Luis Maria Benjamin
author Vaschetto, Luis Maria Benjamin
author_facet Vaschetto, Luis Maria Benjamin
author_role author
dc.subject.none.fl_str_mv CRISPR/CAS9
GENOME EDITING
MINIATURE INVERTED TRANSPOSABLE ELEMENTS
TE-BASED DRIVES
TRANSCRIPTIONAL CONTROL
TRANSPOSABLE ELEMENT AMPLIFICATION
topic CRISPR/CAS9
GENOME EDITING
MINIATURE INVERTED TRANSPOSABLE ELEMENTS
TE-BASED DRIVES
TRANSCRIPTIONAL CONTROL
TRANSPOSABLE ELEMENT AMPLIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited. Nonetheless, TEs have been initially proposed as positive control elements acting in synergy with the host. Nowadays, it is well known that TE movement into host genome comprises an important evolutionary mechanism capable of increasing the adaptive fitness. As insights into TE functioning are increasing day to day, the manipulation of transposition has raised an interesting possibility of setting the host functions, although the lack of appropriate genome engineering tools has unpaved it. Fortunately, the emergence of genome editing technologies based on programmable nucleases, and especially the arrival of a multipurpose RNA-guided Cas9 endonuclease system, has made it possible to reconsider this challenge. For such purpose, a particular type of transposons referred to as miniature inverted-repeat transposable elements (MITEs) has shown a series of interesting characteristics for designing functional drivers. Here, recent insights into MITE elements and versatile RNA-guided CRISPR/Cas9 genome engineering system are given to understand how to deploy the potential of TEs for control of the host transcriptional activity.
Fil: Vaschetto, Luis Maria Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Diversidad Animal I; Argentina
description In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited. Nonetheless, TEs have been initially proposed as positive control elements acting in synergy with the host. Nowadays, it is well known that TE movement into host genome comprises an important evolutionary mechanism capable of increasing the adaptive fitness. As insights into TE functioning are increasing day to day, the manipulation of transposition has raised an interesting possibility of setting the host functions, although the lack of appropriate genome engineering tools has unpaved it. Fortunately, the emergence of genome editing technologies based on programmable nucleases, and especially the arrival of a multipurpose RNA-guided Cas9 endonuclease system, has made it possible to reconsider this challenge. For such purpose, a particular type of transposons referred to as miniature inverted-repeat transposable elements (MITEs) has shown a series of interesting characteristics for designing functional drivers. Here, recent insights into MITE elements and versatile RNA-guided CRISPR/Cas9 genome engineering system are given to understand how to deploy the potential of TEs for control of the host transcriptional activity.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51397
Vaschetto, Luis Maria Benjamin; Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion; Springer; Current Genetics; 64; 2; 4-2018; 405-412
0172-8083
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51397
identifier_str_mv Vaschetto, Luis Maria Benjamin; Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion; Springer; Current Genetics; 64; 2; 4-2018; 405-412
0172-8083
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00294-017-0765-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00294-017-0765-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268716060901376
score 13.13397

Publicaciones similares