Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance
- Autores
- Suguiyama, Vanessa Fuentes; Rodriguez, Jae Diana Paredes; Santos, Tatiane Cristina Nicomedio dos; Lira, Bruno Silvestre; de Haro, Luis Alejandro; Silva, João Paulo Naldi; Borba, Eduardo Leite; Purgatto, Eduardo; Silva, Emerson Alves da; Bellora Pereyra, Nicolás; Carrari, Fernando; Centeno, Danilo da Cruz; Bermudez Salazar, Luisa; Rossi, Magdalena; de Setta, Nathalia
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.
Instituto de Biotecnología
Fil: Suguiyama, Vanessa Fuentes. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil
Fil: Paredes Rodriguez, Jae Diana. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil
Fil: dos Santos, Tatiane Cristina Nicomedio. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil
Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil
Fil: de Haro, Luis Alejandro. Weizmann Institute of Science. Departament of Plant and Environmental Sciences; Israel
Fil: Silva, João Paulo Naldi. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil
Fil: Borba, Eduardo Leite. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Botânica; Brasil
Fil: Purgatto, Eduardo. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Alimentos e Nutrição Experimental; Brasil
Fil: da Silva, Emerson Alves. Secretaria do Meio Ambiente do Estado de São Paulo. Instituto de Botânica; Brasil
Fil: Bellora, Nicolas. Instituto de Tecnologías Nucleares para la Salud (INTECNUS); Argentina
Fil: Bellora, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología Y Biología Molecular; Argentina
Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina
Fil: da Cruz Centeno, Danilo. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil
Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bermudez Salazar, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bermudez Salazar, Luisa. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina
Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil
Fil: de Setta, Nathalia. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil - Fuente
- Plant Molecular Biology 109 : 761-780. (2022)
- Materia
-
Monocotyledons
Transcriptome
Plant Metabolism
Transposons
Phenotypic Plasticity
Tolerance
Monocotiledóneas
Transcriptomas
Metabolismo de las Plantas
Transposones
Plasticidad Fenotípica
Setaria italica
Tolerancia
Water Deficit
Déficit de Agua - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/12491
Ver los metadatos del registro completo
| id |
INTADig_b44aa537686bfbd2ee7331f094fcb02e |
|---|---|
| oai_identifier_str |
oai:localhost:20.500.12123/12491 |
| network_acronym_str |
INTADig |
| repository_id_str |
l |
| network_name_str |
INTA Digital (INTA) |
| spelling |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit toleranceSuguiyama, Vanessa FuentesRodriguez, Jae Diana ParedesSantos, Tatiane Cristina Nicomedio dosLira, Bruno Silvestrede Haro, Luis AlejandroSilva, João Paulo NaldiBorba, Eduardo LeitePurgatto, EduardoSilva, Emerson Alves daBellora Pereyra, NicolásCarrari, FernandoCenteno, Danilo da CruzBermudez Salazar, LuisaRossi, Magdalenade Setta, NathaliaMonocotyledonsTranscriptomePlant MetabolismTransposonsPhenotypic PlasticityToleranceMonocotiledóneasTranscriptomasMetabolismo de las PlantasTransposonesPlasticidad FenotípicaSetaria italicaToleranciaWater DeficitDéficit de AguaDrought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions.Instituto de BiotecnologíaFil: Suguiyama, Vanessa Fuentes. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilFil: Paredes Rodriguez, Jae Diana. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilFil: dos Santos, Tatiane Cristina Nicomedio. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilFil: Lira, Bruno Silvestre. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; BrasilFil: de Haro, Luis Alejandro. Weizmann Institute of Science. Departament of Plant and Environmental Sciences; IsraelFil: Silva, João Paulo Naldi. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilFil: Borba, Eduardo Leite. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Botânica; BrasilFil: Purgatto, Eduardo. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Alimentos e Nutrição Experimental; BrasilFil: da Silva, Emerson Alves. Secretaria do Meio Ambiente do Estado de São Paulo. Instituto de Botânica; BrasilFil: Bellora, Nicolas. Instituto de Tecnologías Nucleares para la Salud (INTECNUS); ArgentinaFil: Bellora, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología Y Biología Molecular; ArgentinaFil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; ArgentinaFil: da Cruz Centeno, Danilo. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilFil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bermudez Salazar, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bermudez Salazar, Luisa. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; ArgentinaFil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; BrasilFil: de Setta, Nathalia. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; BrasilSpringer2022-08-04T11:05:11Z2022-08-04T11:05:11Z2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12491https://link.springer.com/article/10.1007/s11103-022-01273-w1573-50280167-4412https://doi.org/10.1007/s11103-022-01273-wPlant Molecular Biology 109 : 761-780. (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.info:eu-repo/semantics/restrictedAccess2025-09-04T09:49:28Zoai:localhost:20.500.12123/12491instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:28.5INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| spellingShingle |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance Suguiyama, Vanessa Fuentes Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua |
| title_short |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_full |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_fullStr |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_full_unstemmed |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| title_sort |
Regulatory mechanisms behind the phenotypic plasticity associated with Setaria italica water deficit tolerance |
| dc.creator.none.fl_str_mv |
Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia |
| author |
Suguiyama, Vanessa Fuentes |
| author_facet |
Suguiyama, Vanessa Fuentes Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia |
| author_role |
author |
| author2 |
Rodriguez, Jae Diana Paredes Santos, Tatiane Cristina Nicomedio dos Lira, Bruno Silvestre de Haro, Luis Alejandro Silva, João Paulo Naldi Borba, Eduardo Leite Purgatto, Eduardo Silva, Emerson Alves da Bellora Pereyra, Nicolás Carrari, Fernando Centeno, Danilo da Cruz Bermudez Salazar, Luisa Rossi, Magdalena de Setta, Nathalia |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua |
| topic |
Monocotyledons Transcriptome Plant Metabolism Transposons Phenotypic Plasticity Tolerance Monocotiledóneas Transcriptomas Metabolismo de las Plantas Transposones Plasticidad Fenotípica Setaria italica Tolerancia Water Deficit Déficit de Agua |
| dc.description.none.fl_txt_mv |
Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions. Instituto de Biotecnología Fil: Suguiyama, Vanessa Fuentes. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Paredes Rodriguez, Jae Diana. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: dos Santos, Tatiane Cristina Nicomedio. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil Fil: de Haro, Luis Alejandro. Weizmann Institute of Science. Departament of Plant and Environmental Sciences; Israel Fil: Silva, João Paulo Naldi. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Borba, Eduardo Leite. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Botânica; Brasil Fil: Purgatto, Eduardo. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Alimentos e Nutrição Experimental; Brasil Fil: da Silva, Emerson Alves. Secretaria do Meio Ambiente do Estado de São Paulo. Instituto de Botânica; Brasil Fil: Bellora, Nicolas. Instituto de Tecnologías Nucleares para la Salud (INTECNUS); Argentina Fil: Bellora, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología Y Biología Molecular; Argentina Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: da Cruz Centeno, Danilo. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil Fil: Bermudez Salazar, Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bermudez Salazar, Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bermudez Salazar, Luisa. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Genética; Argentina Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociencias. Departamento de Botânica; Brasil Fil: de Setta, Nathalia. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil |
| description |
Drought is one of the main environmental stresses that negatively impacts vegetative and reproductive yield. Water deficit responses are determined by the duration and intensity of the stress, which, together with plant genotype, will define the chances of plant survival. The metabolic adjustments in response to water deficit are complex and involve gene expression modulation regulated by DNA-binding proteins and epigenetic modifications. This last mechanism may also regulate the activity of transposable elements, which in turn impact the expression of nearby loci. Setaria italica plants submitted to five water deficit regimes were analyzed through a phenotypical approach, including growth, physiological, RNA-seq and sRNA-seq analyses. The results showed a progressive reduction in yield as a function of water deficit intensity associated with signaling pathway modulation and metabolic adjustments. We identified a group of loci that were consistently associated with drought responses, some of which were related to water deficit perception, signaling and regulation. Finally, an analysis of the transcriptome and sRNAome allowed us to identify genes putatively regulated by TE- and sRNA-related mechanisms and an intriguing positive correlation between transcript levels and sRNA accumulation in gene body regions. These findings shed light on the processes that allow S. italica to overcome drought and survive under water restrictive conditions. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-08-04T11:05:11Z 2022-08-04T11:05:11Z 2022-05 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/12491 https://link.springer.com/article/10.1007/s11103-022-01273-w 1573-5028 0167-4412 https://doi.org/10.1007/s11103-022-01273-w |
| url |
http://hdl.handle.net/20.500.12123/12491 https://link.springer.com/article/10.1007/s11103-022-01273-w https://doi.org/10.1007/s11103-022-01273-w |
| identifier_str_mv |
1573-5028 0167-4412 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial. |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
| eu_rights_str_mv |
restrictedAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Springer |
| publisher.none.fl_str_mv |
Springer |
| dc.source.none.fl_str_mv |
Plant Molecular Biology 109 : 761-780. (2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
| reponame_str |
INTA Digital (INTA) |
| collection |
INTA Digital (INTA) |
| instname_str |
Instituto Nacional de Tecnología Agropecuaria |
| repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
| repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
| _version_ |
1842341398651600896 |
| score |
12.623145 |