Determination of imidacloprid in beehive samples by UHPLC-MS/MS

Autores
Michlig, Melina Paola; Merke, Julieta; Pacini, Adriana Cecilia; Orellano, Ramiro Emanuel; Beldomenico, Horacio Ramon; Repetti, María Rosa
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg −1 . Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives’ exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO 4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO 4 , PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg −1 ) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey stored approximately 60% of the loaded imidacloprid through syrup feeding.
Fil: Michlig, Melina Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Merke, Julieta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Pacini, Adriana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Orellano, Ramiro Emanuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bees
Beeswax
Honey
Imidacloprid
Quechers
Uhplc-Ms/Ms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82571
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of imidacloprid in beehive samples by UHPLC-MS/MSMichlig, Melina PaolaMerke, JulietaPacini, Adriana CeciliaOrellano, Ramiro EmanuelBeldomenico, Horacio RamonRepetti, María RosaBeesBeeswaxHoneyImidaclopridQuechersUhplc-Ms/Mshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg −1 . Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives’ exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO 4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO 4 , PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg −1 ) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey stored approximately 60% of the loaded imidacloprid through syrup feeding.Fil: Michlig, Melina Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Merke, Julieta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Pacini, Adriana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Orellano, Ramiro Emanuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82571Michlig, Melina Paola; Merke, Julieta; Pacini, Adriana Cecilia; Orellano, Ramiro Emanuel; Beldomenico, Horacio Ramon; et al.; Determination of imidacloprid in beehive samples by UHPLC-MS/MS; Elsevier Science; Microchemical Journal; 143; 12-2018; 72-810026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.07.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303278info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:39Zoai:ri.conicet.gov.ar:11336/82571instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:40.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of imidacloprid in beehive samples by UHPLC-MS/MS
title Determination of imidacloprid in beehive samples by UHPLC-MS/MS
spellingShingle Determination of imidacloprid in beehive samples by UHPLC-MS/MS
Michlig, Melina Paola
Bees
Beeswax
Honey
Imidacloprid
Quechers
Uhplc-Ms/Ms
title_short Determination of imidacloprid in beehive samples by UHPLC-MS/MS
title_full Determination of imidacloprid in beehive samples by UHPLC-MS/MS
title_fullStr Determination of imidacloprid in beehive samples by UHPLC-MS/MS
title_full_unstemmed Determination of imidacloprid in beehive samples by UHPLC-MS/MS
title_sort Determination of imidacloprid in beehive samples by UHPLC-MS/MS
dc.creator.none.fl_str_mv Michlig, Melina Paola
Merke, Julieta
Pacini, Adriana Cecilia
Orellano, Ramiro Emanuel
Beldomenico, Horacio Ramon
Repetti, María Rosa
author Michlig, Melina Paola
author_facet Michlig, Melina Paola
Merke, Julieta
Pacini, Adriana Cecilia
Orellano, Ramiro Emanuel
Beldomenico, Horacio Ramon
Repetti, María Rosa
author_role author
author2 Merke, Julieta
Pacini, Adriana Cecilia
Orellano, Ramiro Emanuel
Beldomenico, Horacio Ramon
Repetti, María Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bees
Beeswax
Honey
Imidacloprid
Quechers
Uhplc-Ms/Ms
topic Bees
Beeswax
Honey
Imidacloprid
Quechers
Uhplc-Ms/Ms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg −1 . Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives’ exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO 4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO 4 , PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg −1 ) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey stored approximately 60% of the loaded imidacloprid through syrup feeding.
Fil: Michlig, Melina Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Merke, Julieta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Pacini, Adriana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Orellano, Ramiro Emanuel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Imidacloprid is a systemic insecticide belonging to the neonicotinoid family. It was the first neonicotinoid introduced in the mid-1990s, and since then, its use has grown rapidly to control pests in a variety of agricultural crops. Several studies have shown that neonicotinoids translocate to the nectar and pollen of treated plants, which represents a potential risk to pollinators. Therefore, an open-field feeding study was carried out. For this purpose, 30 beehives of Apis mellifera L. were installed in the same apiary. All colonies were in similar health and population conditions when assays were started. For seven weeks, colonies were fed with sucrose syrup with different concentrations of imidacloprid: 15, 30, 120 and 240 μg kg −1 . Thus, the assays were divided into four treatments and a witness (Control) with no added imidacloprid. To check the hives’ exposure to imidacloprid and evaluate its distribution, sampling of adult worker bees and larvae was performed before, during and after the whole feeding period (7 weeks). Furthermore, in the 15th week, honey and beeswax (honeycomb) samples were collected from the brood chamber and honey super of all hives. Analytical methodologies for sample preparation based on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) procedure were optimized and validated. After soaking the bees and honey samples and the extraction using acetonitrile with MgSO 4 and NaCl salts, a dispersive solid-phase extraction (d-SPE) step with MgSO 4 , PSA and C18 was applied. Melted beeswax was subjected to an acetonitrile extraction, followed by freeze-out and d-SPE with PSA and C18. Extracts were evaluated in a UHPLC-MS/MS system. LOQ (μg kg −1 ) values were 0.25, 0.50 and 1 for honey, bees and beeswax, respectively. Satisfactory recovery performance was achieved with relative standard deviation ≤20%. Residue concentrations of imidacloprid in samples showed correlation with the doses supplied, indicating exposure of the beehives to the insecticide. Honey stored approximately 60% of the loaded imidacloprid through syrup feeding.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82571
Michlig, Melina Paola; Merke, Julieta; Pacini, Adriana Cecilia; Orellano, Ramiro Emanuel; Beldomenico, Horacio Ramon; et al.; Determination of imidacloprid in beehive samples by UHPLC-MS/MS; Elsevier Science; Microchemical Journal; 143; 12-2018; 72-81
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82571
identifier_str_mv Michlig, Melina Paola; Merke, Julieta; Pacini, Adriana Cecilia; Orellano, Ramiro Emanuel; Beldomenico, Horacio Ramon; et al.; Determination of imidacloprid in beehive samples by UHPLC-MS/MS; Elsevier Science; Microchemical Journal; 143; 12-2018; 72-81
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.07.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303278
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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