Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk

Autores
Michlig, Nicolás; Repetti, María Rosa; Chiericatti, Carolina; García, Silvia R.; Gaggiotti, Mónica; Basilico, Juan Carlos; Beldomenico, Horacio Ramon
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
Fil: Chiericatti, Carolina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: García, Silvia R.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
Fil: Gaggiotti, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Basilico, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
Materia
Lc-Ms/Ms
Quechers
Mycotoxins
Milk
Pesticides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45964
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milkMichlig, NicolásRepetti, María RosaChiericatti, CarolinaGarcía, Silvia R.Gaggiotti, MónicaBasilico, Juan CarlosBeldomenico, Horacio RamonLc-Ms/MsQuechersMycotoxinsMilkPesticideshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Chiericatti, Carolina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: García, Silvia R.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaFil: Gaggiotti, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Basilico, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; ArgentinaFil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45964Michlig, Nicolás; Repetti, María Rosa; Chiericatti, Carolina; García, Silvia R.; Gaggiotti, Mónica; et al.; Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk; Springer; Chromatographia; 79; 17-18; 10-2015; 1091-11000009-5893CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10337-015-2972-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10337-015-2972-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:44Zoai:ri.conicet.gov.ar:11336/45964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:44.637CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
title Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
spellingShingle Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
Michlig, Nicolás
Lc-Ms/Ms
Quechers
Mycotoxins
Milk
Pesticides
title_short Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
title_full Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
title_fullStr Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
title_full_unstemmed Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
title_sort Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk
dc.creator.none.fl_str_mv Michlig, Nicolás
Repetti, María Rosa
Chiericatti, Carolina
García, Silvia R.
Gaggiotti, Mónica
Basilico, Juan Carlos
Beldomenico, Horacio Ramon
author Michlig, Nicolás
author_facet Michlig, Nicolás
Repetti, María Rosa
Chiericatti, Carolina
García, Silvia R.
Gaggiotti, Mónica
Basilico, Juan Carlos
Beldomenico, Horacio Ramon
author_role author
author2 Repetti, María Rosa
Chiericatti, Carolina
García, Silvia R.
Gaggiotti, Mónica
Basilico, Juan Carlos
Beldomenico, Horacio Ramon
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Lc-Ms/Ms
Quechers
Mycotoxins
Milk
Pesticides
topic Lc-Ms/Ms
Quechers
Mycotoxins
Milk
Pesticides
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.
Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
Fil: Chiericatti, Carolina. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: García, Silvia R.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
Fil: Gaggiotti, Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Basilico, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química; Argentina
Fil: Beldomenico, Horacio Ramon. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina
description A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45964
Michlig, Nicolás; Repetti, María Rosa; Chiericatti, Carolina; García, Silvia R.; Gaggiotti, Mónica; et al.; Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk; Springer; Chromatographia; 79; 17-18; 10-2015; 1091-1100
0009-5893
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45964
identifier_str_mv Michlig, Nicolás; Repetti, María Rosa; Chiericatti, Carolina; García, Silvia R.; Gaggiotti, Mónica; et al.; Multiclass compatible sample preparation for UHPLC-MS/MS determination of aflatoxin M1 in raw milk; Springer; Chromatographia; 79; 17-18; 10-2015; 1091-1100
0009-5893
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10337-015-2972-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10337-015-2972-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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