Genome rearrangements derived from autopolyploidization in Paspalum sp.

Autores
Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; Pessino, Silvina Claudia
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rapid sequence rearrangements along with alterations in cytosine-directed methylation patterns follow allopolyploid formation in several plant species. The occurrence of similar changes is yet to be examined in detail during the generation of autopolyploids. The objective of this work was to investigate the presence of nascent variation in genome sequence after autopolyploidization in two Paspalum species. The increments of ploidy level were produced either by hybridization involving unreduced gametes or colchicine treatment. Genetic modifications affected 15–23% and 9.55% of the genomic loci in Paspalum rufum and Paspalum notatum, respectively. In all cases the frequency of band loss from the progenitors was significantly higher than that of novel band gain. In random amplified polymorphic DNA (RAPD) experiments the majority of polymorphisms were detected by particular sets of primers, suggesting that repetitive domains were preferentially targeted. Several P. notatum polymorphic fragments were cloned, sequenced and used as probes in genomic hybridization analyses. Some of them corresponded to interspersed middle-repetitive sequences with unknown function. Methylation-sensitive genomic hybridization showed that sequence modifications occurred invariably in cytosine-methylated regions, which remained methylated after polyploidization.
Fil: Martelotto, Luciano G.. Universidad Nacional de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Stein, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
Autoploidy
Genome Evolution
Genome Variation
Molecular Markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46853
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46853
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome rearrangements derived from autopolyploidization in Paspalum sp.Martelotto, Luciano G.Ortiz, Juan Pablo AmelioStein, JulianaEspinoza, FranciscoQuarin, Camilo LuisPessino, Silvina ClaudiaAutoploidyGenome EvolutionGenome VariationMolecular Markershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rapid sequence rearrangements along with alterations in cytosine-directed methylation patterns follow allopolyploid formation in several plant species. The occurrence of similar changes is yet to be examined in detail during the generation of autopolyploids. The objective of this work was to investigate the presence of nascent variation in genome sequence after autopolyploidization in two Paspalum species. The increments of ploidy level were produced either by hybridization involving unreduced gametes or colchicine treatment. Genetic modifications affected 15–23% and 9.55% of the genomic loci in Paspalum rufum and Paspalum notatum, respectively. In all cases the frequency of band loss from the progenitors was significantly higher than that of novel band gain. In random amplified polymorphic DNA (RAPD) experiments the majority of polymorphisms were detected by particular sets of primers, suggesting that repetitive domains were preferentially targeted. Several P. notatum polymorphic fragments were cloned, sequenced and used as probes in genomic hybridization analyses. Some of them corresponded to interspersed middle-repetitive sequences with unknown function. Methylation-sensitive genomic hybridization showed that sequence modifications occurred invariably in cytosine-methylated regions, which remained methylated after polyploidization.Fil: Martelotto, Luciano G.. Universidad Nacional de Rosario; ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Stein, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaElsevier Ireland2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46853Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; Genome rearrangements derived from autopolyploidization in Paspalum sp.; Elsevier Ireland; Plant Science; 172; 5; 12-2007; 970-9770168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2007.02.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945207000520info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:29Zoai:ri.conicet.gov.ar:11336/46853instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:29.626CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome rearrangements derived from autopolyploidization in Paspalum sp.
title Genome rearrangements derived from autopolyploidization in Paspalum sp.
spellingShingle Genome rearrangements derived from autopolyploidization in Paspalum sp.
Martelotto, Luciano G.
Autoploidy
Genome Evolution
Genome Variation
Molecular Markers
title_short Genome rearrangements derived from autopolyploidization in Paspalum sp.
title_full Genome rearrangements derived from autopolyploidization in Paspalum sp.
title_fullStr Genome rearrangements derived from autopolyploidization in Paspalum sp.
title_full_unstemmed Genome rearrangements derived from autopolyploidization in Paspalum sp.
title_sort Genome rearrangements derived from autopolyploidization in Paspalum sp.
dc.creator.none.fl_str_mv Martelotto, Luciano G.
Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author Martelotto, Luciano G.
author_facet Martelotto, Luciano G.
Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author_role author
author2 Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Autoploidy
Genome Evolution
Genome Variation
Molecular Markers
topic Autoploidy
Genome Evolution
Genome Variation
Molecular Markers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rapid sequence rearrangements along with alterations in cytosine-directed methylation patterns follow allopolyploid formation in several plant species. The occurrence of similar changes is yet to be examined in detail during the generation of autopolyploids. The objective of this work was to investigate the presence of nascent variation in genome sequence after autopolyploidization in two Paspalum species. The increments of ploidy level were produced either by hybridization involving unreduced gametes or colchicine treatment. Genetic modifications affected 15–23% and 9.55% of the genomic loci in Paspalum rufum and Paspalum notatum, respectively. In all cases the frequency of band loss from the progenitors was significantly higher than that of novel band gain. In random amplified polymorphic DNA (RAPD) experiments the majority of polymorphisms were detected by particular sets of primers, suggesting that repetitive domains were preferentially targeted. Several P. notatum polymorphic fragments were cloned, sequenced and used as probes in genomic hybridization analyses. Some of them corresponded to interspersed middle-repetitive sequences with unknown function. Methylation-sensitive genomic hybridization showed that sequence modifications occurred invariably in cytosine-methylated regions, which remained methylated after polyploidization.
Fil: Martelotto, Luciano G.. Universidad Nacional de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Stein, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Rapid sequence rearrangements along with alterations in cytosine-directed methylation patterns follow allopolyploid formation in several plant species. The occurrence of similar changes is yet to be examined in detail during the generation of autopolyploids. The objective of this work was to investigate the presence of nascent variation in genome sequence after autopolyploidization in two Paspalum species. The increments of ploidy level were produced either by hybridization involving unreduced gametes or colchicine treatment. Genetic modifications affected 15–23% and 9.55% of the genomic loci in Paspalum rufum and Paspalum notatum, respectively. In all cases the frequency of band loss from the progenitors was significantly higher than that of novel band gain. In random amplified polymorphic DNA (RAPD) experiments the majority of polymorphisms were detected by particular sets of primers, suggesting that repetitive domains were preferentially targeted. Several P. notatum polymorphic fragments were cloned, sequenced and used as probes in genomic hybridization analyses. Some of them corresponded to interspersed middle-repetitive sequences with unknown function. Methylation-sensitive genomic hybridization showed that sequence modifications occurred invariably in cytosine-methylated regions, which remained methylated after polyploidization.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46853
Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; Genome rearrangements derived from autopolyploidization in Paspalum sp.; Elsevier Ireland; Plant Science; 172; 5; 12-2007; 970-977
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46853
identifier_str_mv Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; Genome rearrangements derived from autopolyploidization in Paspalum sp.; Elsevier Ireland; Plant Science; 172; 5; 12-2007; 970-977
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2007.02.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945207000520
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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