Histologic effect of a postnatal slow-release GnRH agonist on feline gonads

Autores
Carranza Martin, Ana Cristina; Faya, Marcela Inés; Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Gobello, María Cristina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In postnatal domestic cats, GnRH agonists suppressed fecal concentrations of sexual steroids and delayed puberty. The aim of this study was to describe the gross and microscopic morphometric effects of a single administration of the GnRH agonist, deslorelin acetate, on the gonads of postnatally treated cats. Twenty-seven postnatal male (n=14) and female (n=13) kittens were randomly assigned to one of the following treatment groups within the first 24hours of birth: deslorelin acetate (1.6mg, subcutaneous; DA, n=16) or control that remained untreated (CO, n=11) and spayed or castrated immediately after the onset of puberty. After surgical removal, the gonads were gross and histologically assessed. Sertoli cells also were examined immunohistochemically. Comparisons between the treatments were carried out by the Student t test. Gross gonadal wet weight and volume as well as gonadosomatic index were significantly lower in the DA than those in the CO males; these same parameters were not different in females. Primordial (461.4+3.0 vs. 1074.3+117.5; P<0.01), primary (59.1+13.5 vs. 165.4+24.6; P<0.01), and secondary (17.5+2.6 vs. 31.17+8.1; P<0.05) follicles per mm2 were decreased in DA than in CO gonads. Epididymal sperm motility and morphology were normal in all but two DA cats that had too few sperm to be evaluated. Germinal epithelial height (μm; 39.68+0.92 vs. 72.7+1.2; P<0.01) and most of their cellular components as well as the Sertoli (cm3; 0.1+0.02 vs. 0.24+0.05; P<0.01) cells were diminished in the DA cats. Gonadotropin-releasing hormone agonist endocrine disruption during the neonatal critical reproductive time window may have a potential as a contraceptive agent in domestic felids.
Fil: Carranza Martin, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faya, Marcela Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Patricia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Feline
Gnrh Agonist
Gonad
Neonatal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53606
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53606
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histologic effect of a postnatal slow-release GnRH agonist on feline gonadsCarranza Martin, Ana CristinaFaya, Marcela InésFernández, Patricia ElenaBarbeito, Claudio GustavoGobello, María CristinaFelineGnrh AgonistGonadNeonatalhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In postnatal domestic cats, GnRH agonists suppressed fecal concentrations of sexual steroids and delayed puberty. The aim of this study was to describe the gross and microscopic morphometric effects of a single administration of the GnRH agonist, deslorelin acetate, on the gonads of postnatally treated cats. Twenty-seven postnatal male (n=14) and female (n=13) kittens were randomly assigned to one of the following treatment groups within the first 24hours of birth: deslorelin acetate (1.6mg, subcutaneous; DA, n=16) or control that remained untreated (CO, n=11) and spayed or castrated immediately after the onset of puberty. After surgical removal, the gonads were gross and histologically assessed. Sertoli cells also were examined immunohistochemically. Comparisons between the treatments were carried out by the Student t test. Gross gonadal wet weight and volume as well as gonadosomatic index were significantly lower in the DA than those in the CO males; these same parameters were not different in females. Primordial (461.4+3.0 vs. 1074.3+117.5; P<0.01), primary (59.1+13.5 vs. 165.4+24.6; P<0.01), and secondary (17.5+2.6 vs. 31.17+8.1; P<0.05) follicles per mm2 were decreased in DA than in CO gonads. Epididymal sperm motility and morphology were normal in all but two DA cats that had too few sperm to be evaluated. Germinal epithelial height (μm; 39.68+0.92 vs. 72.7+1.2; P<0.01) and most of their cellular components as well as the Sertoli (cm3; 0.1+0.02 vs. 0.24+0.05; P<0.01) cells were diminished in the DA cats. Gonadotropin-releasing hormone agonist endocrine disruption during the neonatal critical reproductive time window may have a potential as a contraceptive agent in domestic felids.Fil: Carranza Martin, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Faya, Marcela Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández, Patricia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Inc2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53606Carranza Martin, Ana Cristina; Faya, Marcela Inés; Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Gobello, María Cristina; Histologic effect of a postnatal slow-release GnRH agonist on feline gonads; Elsevier Science Inc; Theriogenology; 83; 8; 5-2015; 1368-13720093-691XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.01.027info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15000473info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:40Zoai:ri.conicet.gov.ar:11336/53606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:41.012CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
title Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
spellingShingle Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
Carranza Martin, Ana Cristina
Feline
Gnrh Agonist
Gonad
Neonatal
title_short Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
title_full Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
title_fullStr Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
title_full_unstemmed Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
title_sort Histologic effect of a postnatal slow-release GnRH agonist on feline gonads
dc.creator.none.fl_str_mv Carranza Martin, Ana Cristina
Faya, Marcela Inés
Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Gobello, María Cristina
author Carranza Martin, Ana Cristina
author_facet Carranza Martin, Ana Cristina
Faya, Marcela Inés
Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Gobello, María Cristina
author_role author
author2 Faya, Marcela Inés
Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Gobello, María Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Feline
Gnrh Agonist
Gonad
Neonatal
topic Feline
Gnrh Agonist
Gonad
Neonatal
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In postnatal domestic cats, GnRH agonists suppressed fecal concentrations of sexual steroids and delayed puberty. The aim of this study was to describe the gross and microscopic morphometric effects of a single administration of the GnRH agonist, deslorelin acetate, on the gonads of postnatally treated cats. Twenty-seven postnatal male (n=14) and female (n=13) kittens were randomly assigned to one of the following treatment groups within the first 24hours of birth: deslorelin acetate (1.6mg, subcutaneous; DA, n=16) or control that remained untreated (CO, n=11) and spayed or castrated immediately after the onset of puberty. After surgical removal, the gonads were gross and histologically assessed. Sertoli cells also were examined immunohistochemically. Comparisons between the treatments were carried out by the Student t test. Gross gonadal wet weight and volume as well as gonadosomatic index were significantly lower in the DA than those in the CO males; these same parameters were not different in females. Primordial (461.4+3.0 vs. 1074.3+117.5; P<0.01), primary (59.1+13.5 vs. 165.4+24.6; P<0.01), and secondary (17.5+2.6 vs. 31.17+8.1; P<0.05) follicles per mm2 were decreased in DA than in CO gonads. Epididymal sperm motility and morphology were normal in all but two DA cats that had too few sperm to be evaluated. Germinal epithelial height (μm; 39.68+0.92 vs. 72.7+1.2; P<0.01) and most of their cellular components as well as the Sertoli (cm3; 0.1+0.02 vs. 0.24+0.05; P<0.01) cells were diminished in the DA cats. Gonadotropin-releasing hormone agonist endocrine disruption during the neonatal critical reproductive time window may have a potential as a contraceptive agent in domestic felids.
Fil: Carranza Martin, Ana Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faya, Marcela Inés. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Patricia Elena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gobello, María Cristina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In postnatal domestic cats, GnRH agonists suppressed fecal concentrations of sexual steroids and delayed puberty. The aim of this study was to describe the gross and microscopic morphometric effects of a single administration of the GnRH agonist, deslorelin acetate, on the gonads of postnatally treated cats. Twenty-seven postnatal male (n=14) and female (n=13) kittens were randomly assigned to one of the following treatment groups within the first 24hours of birth: deslorelin acetate (1.6mg, subcutaneous; DA, n=16) or control that remained untreated (CO, n=11) and spayed or castrated immediately after the onset of puberty. After surgical removal, the gonads were gross and histologically assessed. Sertoli cells also were examined immunohistochemically. Comparisons between the treatments were carried out by the Student t test. Gross gonadal wet weight and volume as well as gonadosomatic index were significantly lower in the DA than those in the CO males; these same parameters were not different in females. Primordial (461.4+3.0 vs. 1074.3+117.5; P<0.01), primary (59.1+13.5 vs. 165.4+24.6; P<0.01), and secondary (17.5+2.6 vs. 31.17+8.1; P<0.05) follicles per mm2 were decreased in DA than in CO gonads. Epididymal sperm motility and morphology were normal in all but two DA cats that had too few sperm to be evaluated. Germinal epithelial height (μm; 39.68+0.92 vs. 72.7+1.2; P<0.01) and most of their cellular components as well as the Sertoli (cm3; 0.1+0.02 vs. 0.24+0.05; P<0.01) cells were diminished in the DA cats. Gonadotropin-releasing hormone agonist endocrine disruption during the neonatal critical reproductive time window may have a potential as a contraceptive agent in domestic felids.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53606
Carranza Martin, Ana Cristina; Faya, Marcela Inés; Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Gobello, María Cristina; Histologic effect of a postnatal slow-release GnRH agonist on feline gonads; Elsevier Science Inc; Theriogenology; 83; 8; 5-2015; 1368-1372
0093-691X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53606
identifier_str_mv Carranza Martin, Ana Cristina; Faya, Marcela Inés; Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Gobello, María Cristina; Histologic effect of a postnatal slow-release GnRH agonist on feline gonads; Elsevier Science Inc; Theriogenology; 83; 8; 5-2015; 1368-1372
0093-691X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2015.01.027
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X15000473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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