An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line

Autores
Marino, Gabriela Inés; Assef, Yanina Andrea; Kotsias, Basilio Aristides
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, an outwardly rectifying chloride channel was characterized in the trophoblastic cell line BeWo, a human hormone-synthesizing cell which displays many biochemical and morphological properties similar to those reported for the human cytotrophoblast. Ion channel activity was recorded in the cell attached and inside-out configurations with standard patch-clamp technology. In most of the BeWo cells studied, the channel under symmetrical N-methyl-d-glucamine (NMDG-Cl) concentration (Na+ free solution) in both sides of the membrane exhibited spontaneous activity, an outwardly rectifying current/voltage relationship and single-channel conductances of 15 pS and 48 pS for inwards and outwards currents, respectively. The channel has a low permeability for gluconate with a relative permeability Pgluconate/PCl of 0.23, and a higher permeability to I−. The open probability (Po) of the channel exhibited dependence with the applied membrane potential with greater activity at positive pulses. The channel activity was inhibited by the sulphonylurea hypoglycemic agent glibenclamide (50 μM) or by diphenylamine-2-carboxylate (DPC, 500 μM) added to the cytoplasmic side of the patch whereas conductances remained unchanged. The blockade with glibenclamide and DPC was independent of the applied membrane potential. All these results are characteristic of the outwardly rectifying Cl channel (ORCC) found in other types of cells. Neither Po, conductances nor reversal potential (Er) values were affected by the absence of intracellular Ca2+, suggesting that the channel is not sensitive to Ca2+.
Fil: Marino, Gabriela Inés. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
Fil: Assef, Yanina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
Fil: Kotsias, Basilio Aristides. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
Materia
Orcc
Bewo Cells
Trophoblastic Cells
Ion Channels
Patch Clamp
Glibenclamide
Dpc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15048
Acceder
id CONICETDig_2546da4e542cade1c56305be78117ef6
oai_identifier_str oai:ri.conicet.gov.ar:11336/15048
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An outwardly rectifying chloride channel in BeWo choriocarcinoma cell lineMarino, Gabriela InésAssef, Yanina AndreaKotsias, Basilio AristidesOrccBewo CellsTrophoblastic CellsIon ChannelsPatch ClampGlibenclamideDpchttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1In this study, an outwardly rectifying chloride channel was characterized in the trophoblastic cell line BeWo, a human hormone-synthesizing cell which displays many biochemical and morphological properties similar to those reported for the human cytotrophoblast. Ion channel activity was recorded in the cell attached and inside-out configurations with standard patch-clamp technology. In most of the BeWo cells studied, the channel under symmetrical N-methyl-d-glucamine (NMDG-Cl) concentration (Na+ free solution) in both sides of the membrane exhibited spontaneous activity, an outwardly rectifying current/voltage relationship and single-channel conductances of 15 pS and 48 pS for inwards and outwards currents, respectively. The channel has a low permeability for gluconate with a relative permeability Pgluconate/PCl of 0.23, and a higher permeability to I−. The open probability (Po) of the channel exhibited dependence with the applied membrane potential with greater activity at positive pulses. The channel activity was inhibited by the sulphonylurea hypoglycemic agent glibenclamide (50 μM) or by diphenylamine-2-carboxylate (DPC, 500 μM) added to the cytoplasmic side of the patch whereas conductances remained unchanged. The blockade with glibenclamide and DPC was independent of the applied membrane potential. All these results are characteristic of the outwardly rectifying Cl channel (ORCC) found in other types of cells. Neither Po, conductances nor reversal potential (Er) values were affected by the absence of intracellular Ca2+, suggesting that the channel is not sensitive to Ca2+.Fil: Marino, Gabriela Inés. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; ArgentinaFil: Assef, Yanina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; ArgentinaFil: Kotsias, Basilio Aristides. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; ArgentinaElsevier2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15048Marino, Gabriela Inés; Assef, Yanina Andrea; Kotsias, Basilio Aristides; An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line; Elsevier; Placenta; 31; 12; 12-2010; 1093-11000143-4004enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0143400410003632info:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2010.09.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:52Zoai:ri.conicet.gov.ar:11336/15048instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:52.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
title An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
spellingShingle An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
Marino, Gabriela Inés
Orcc
Bewo Cells
Trophoblastic Cells
Ion Channels
Patch Clamp
Glibenclamide
Dpc
title_short An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
title_full An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
title_fullStr An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
title_full_unstemmed An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
title_sort An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line
dc.creator.none.fl_str_mv Marino, Gabriela Inés
Assef, Yanina Andrea
Kotsias, Basilio Aristides
author Marino, Gabriela Inés
author_facet Marino, Gabriela Inés
Assef, Yanina Andrea
Kotsias, Basilio Aristides
author_role author
author2 Assef, Yanina Andrea
Kotsias, Basilio Aristides
author2_role author
author
dc.subject.none.fl_str_mv Orcc
Bewo Cells
Trophoblastic Cells
Ion Channels
Patch Clamp
Glibenclamide
Dpc
topic Orcc
Bewo Cells
Trophoblastic Cells
Ion Channels
Patch Clamp
Glibenclamide
Dpc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, an outwardly rectifying chloride channel was characterized in the trophoblastic cell line BeWo, a human hormone-synthesizing cell which displays many biochemical and morphological properties similar to those reported for the human cytotrophoblast. Ion channel activity was recorded in the cell attached and inside-out configurations with standard patch-clamp technology. In most of the BeWo cells studied, the channel under symmetrical N-methyl-d-glucamine (NMDG-Cl) concentration (Na+ free solution) in both sides of the membrane exhibited spontaneous activity, an outwardly rectifying current/voltage relationship and single-channel conductances of 15 pS and 48 pS for inwards and outwards currents, respectively. The channel has a low permeability for gluconate with a relative permeability Pgluconate/PCl of 0.23, and a higher permeability to I−. The open probability (Po) of the channel exhibited dependence with the applied membrane potential with greater activity at positive pulses. The channel activity was inhibited by the sulphonylurea hypoglycemic agent glibenclamide (50 μM) or by diphenylamine-2-carboxylate (DPC, 500 μM) added to the cytoplasmic side of the patch whereas conductances remained unchanged. The blockade with glibenclamide and DPC was independent of the applied membrane potential. All these results are characteristic of the outwardly rectifying Cl channel (ORCC) found in other types of cells. Neither Po, conductances nor reversal potential (Er) values were affected by the absence of intracellular Ca2+, suggesting that the channel is not sensitive to Ca2+.
Fil: Marino, Gabriela Inés. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
Fil: Assef, Yanina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
Fil: Kotsias, Basilio Aristides. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina
description In this study, an outwardly rectifying chloride channel was characterized in the trophoblastic cell line BeWo, a human hormone-synthesizing cell which displays many biochemical and morphological properties similar to those reported for the human cytotrophoblast. Ion channel activity was recorded in the cell attached and inside-out configurations with standard patch-clamp technology. In most of the BeWo cells studied, the channel under symmetrical N-methyl-d-glucamine (NMDG-Cl) concentration (Na+ free solution) in both sides of the membrane exhibited spontaneous activity, an outwardly rectifying current/voltage relationship and single-channel conductances of 15 pS and 48 pS for inwards and outwards currents, respectively. The channel has a low permeability for gluconate with a relative permeability Pgluconate/PCl of 0.23, and a higher permeability to I−. The open probability (Po) of the channel exhibited dependence with the applied membrane potential with greater activity at positive pulses. The channel activity was inhibited by the sulphonylurea hypoglycemic agent glibenclamide (50 μM) or by diphenylamine-2-carboxylate (DPC, 500 μM) added to the cytoplasmic side of the patch whereas conductances remained unchanged. The blockade with glibenclamide and DPC was independent of the applied membrane potential. All these results are characteristic of the outwardly rectifying Cl channel (ORCC) found in other types of cells. Neither Po, conductances nor reversal potential (Er) values were affected by the absence of intracellular Ca2+, suggesting that the channel is not sensitive to Ca2+.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15048
Marino, Gabriela Inés; Assef, Yanina Andrea; Kotsias, Basilio Aristides; An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line; Elsevier; Placenta; 31; 12; 12-2010; 1093-1100
0143-4004
url http://hdl.handle.net/11336/15048
identifier_str_mv Marino, Gabriela Inés; Assef, Yanina Andrea; Kotsias, Basilio Aristides; An outwardly rectifying chloride channel in BeWo choriocarcinoma cell line; Elsevier; Placenta; 31; 12; 12-2010; 1093-1100
0143-4004
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0143400410003632
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2010.09.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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