K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production

Autores
Corradi, Jeremias; Thompson, Benjamin; Fletcher, Patrick A.; Bertram, Richard; Sherman, Arthur S.; Satin, Leslie S.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pancreatic beta cells secrete insulin in response to plasma glucose. The ATP-sensitive potassium channel (KATP) links glucose metabolism to islet electrical activity in these cells by responding to increased cytosolic [ATP]/[ADP]. It was recently proposed that pyruvate kinase (PK) in close proximity to beta cell KATP locally produces the ATP that inhibits KATP activity. This proposal was largely based on the observation that applying phosphoenolpyruvate (PEP) and ADP to the cytoplasmic side of excised inside-out patches inhibited KATP. To test the relative contributions of local vs. mitochondrial ATP production, we recorded KATP activity using mouse beta cells and INS-1 832/13 cells. In contrast to prior reports, we could not replicate inhibition of KATP activity by PEP + ADP. However, when the pH of the PEP solutions was not corrected for the addition of PEP, strong channel inhibition was observed as a result of the well-known action of protons to inhibit KATP. In cell-attached recordings, perifusing either a PK activator or an inhibitor had little or no effect on KATP channel closure by glucose, further suggesting that PK is not an important regulator of KATP. In contrast, addition of mitochondrial inhibitors robustly increased KATP activity. Finally, by measuring the [ATP]/[ADP] responses to imposed calcium oscillations in mouse beta cells, we found that oxidative phosphorylation could raise [ATP]/[ADP] even when ADP was at its nadir during the burst silent phase, in agreement with our mathematical model. These results indicate that ATP produced by mitochondrial oxidative phosphorylation is the primary controller of KATP in pancreatic beta cells
Fil: Corradi, Jeremias. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Thompson, Benjamin. University of Michigan; Estados Unidos
Fil: Fletcher, Patrick A.. National Institutes of Health; Estados Unidos
Fil: Bertram, Richard. Florida State University; Estados Unidos
Fil: Sherman, Arthur S.. National Institutes of Health; Estados Unidos
Fil: Satin, Leslie S.. University of Michigan; Estados Unidos
Materia
DIABETES
PATCH-CLAMP
BETA CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/239102

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network_name_str CONICET Digital (CONICET)
spelling K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP productionCorradi, JeremiasThompson, BenjaminFletcher, Patrick A.Bertram, RichardSherman, Arthur S.Satin, Leslie S.DIABETESPATCH-CLAMPBETA CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pancreatic beta cells secrete insulin in response to plasma glucose. The ATP-sensitive potassium channel (KATP) links glucose metabolism to islet electrical activity in these cells by responding to increased cytosolic [ATP]/[ADP]. It was recently proposed that pyruvate kinase (PK) in close proximity to beta cell KATP locally produces the ATP that inhibits KATP activity. This proposal was largely based on the observation that applying phosphoenolpyruvate (PEP) and ADP to the cytoplasmic side of excised inside-out patches inhibited KATP. To test the relative contributions of local vs. mitochondrial ATP production, we recorded KATP activity using mouse beta cells and INS-1 832/13 cells. In contrast to prior reports, we could not replicate inhibition of KATP activity by PEP + ADP. However, when the pH of the PEP solutions was not corrected for the addition of PEP, strong channel inhibition was observed as a result of the well-known action of protons to inhibit KATP. In cell-attached recordings, perifusing either a PK activator or an inhibitor had little or no effect on KATP channel closure by glucose, further suggesting that PK is not an important regulator of KATP. In contrast, addition of mitochondrial inhibitors robustly increased KATP activity. Finally, by measuring the [ATP]/[ADP] responses to imposed calcium oscillations in mouse beta cells, we found that oxidative phosphorylation could raise [ATP]/[ADP] even when ADP was at its nadir during the burst silent phase, in agreement with our mathematical model. These results indicate that ATP produced by mitochondrial oxidative phosphorylation is the primary controller of KATP in pancreatic beta cellsFil: Corradi, Jeremias. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Thompson, Benjamin. University of Michigan; Estados UnidosFil: Fletcher, Patrick A.. National Institutes of Health; Estados UnidosFil: Bertram, Richard. Florida State University; Estados UnidosFil: Sherman, Arthur S.. National Institutes of Health; Estados UnidosFil: Satin, Leslie S.. University of Michigan; Estados UnidosJohn Wiley & Sons Inc.2023-11-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/239102Corradi, Jeremias; Thompson, Benjamin; Fletcher, Patrick A.; Bertram, Richard; Sherman, Arthur S.; et al.; K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production; John Wiley & Sons Inc.; The Journal of Physiology; 601; 24; 17-11-2023; 5655-56671469-77930022-3751CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP284982info:eu-repo/semantics/altIdentifier/doi/10.1113/JP284982info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:10Zoai:ri.conicet.gov.ar:11336/239102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:10.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
title K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
spellingShingle K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
Corradi, Jeremias
DIABETES
PATCH-CLAMP
BETA CELLS
title_short K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
title_full K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
title_fullStr K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
title_full_unstemmed K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
title_sort K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production
dc.creator.none.fl_str_mv Corradi, Jeremias
Thompson, Benjamin
Fletcher, Patrick A.
Bertram, Richard
Sherman, Arthur S.
Satin, Leslie S.
author Corradi, Jeremias
author_facet Corradi, Jeremias
Thompson, Benjamin
Fletcher, Patrick A.
Bertram, Richard
Sherman, Arthur S.
Satin, Leslie S.
author_role author
author2 Thompson, Benjamin
Fletcher, Patrick A.
Bertram, Richard
Sherman, Arthur S.
Satin, Leslie S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DIABETES
PATCH-CLAMP
BETA CELLS
topic DIABETES
PATCH-CLAMP
BETA CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pancreatic beta cells secrete insulin in response to plasma glucose. The ATP-sensitive potassium channel (KATP) links glucose metabolism to islet electrical activity in these cells by responding to increased cytosolic [ATP]/[ADP]. It was recently proposed that pyruvate kinase (PK) in close proximity to beta cell KATP locally produces the ATP that inhibits KATP activity. This proposal was largely based on the observation that applying phosphoenolpyruvate (PEP) and ADP to the cytoplasmic side of excised inside-out patches inhibited KATP. To test the relative contributions of local vs. mitochondrial ATP production, we recorded KATP activity using mouse beta cells and INS-1 832/13 cells. In contrast to prior reports, we could not replicate inhibition of KATP activity by PEP + ADP. However, when the pH of the PEP solutions was not corrected for the addition of PEP, strong channel inhibition was observed as a result of the well-known action of protons to inhibit KATP. In cell-attached recordings, perifusing either a PK activator or an inhibitor had little or no effect on KATP channel closure by glucose, further suggesting that PK is not an important regulator of KATP. In contrast, addition of mitochondrial inhibitors robustly increased KATP activity. Finally, by measuring the [ATP]/[ADP] responses to imposed calcium oscillations in mouse beta cells, we found that oxidative phosphorylation could raise [ATP]/[ADP] even when ADP was at its nadir during the burst silent phase, in agreement with our mathematical model. These results indicate that ATP produced by mitochondrial oxidative phosphorylation is the primary controller of KATP in pancreatic beta cells
Fil: Corradi, Jeremias. University of Michigan; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Thompson, Benjamin. University of Michigan; Estados Unidos
Fil: Fletcher, Patrick A.. National Institutes of Health; Estados Unidos
Fil: Bertram, Richard. Florida State University; Estados Unidos
Fil: Sherman, Arthur S.. National Institutes of Health; Estados Unidos
Fil: Satin, Leslie S.. University of Michigan; Estados Unidos
description Pancreatic beta cells secrete insulin in response to plasma glucose. The ATP-sensitive potassium channel (KATP) links glucose metabolism to islet electrical activity in these cells by responding to increased cytosolic [ATP]/[ADP]. It was recently proposed that pyruvate kinase (PK) in close proximity to beta cell KATP locally produces the ATP that inhibits KATP activity. This proposal was largely based on the observation that applying phosphoenolpyruvate (PEP) and ADP to the cytoplasmic side of excised inside-out patches inhibited KATP. To test the relative contributions of local vs. mitochondrial ATP production, we recorded KATP activity using mouse beta cells and INS-1 832/13 cells. In contrast to prior reports, we could not replicate inhibition of KATP activity by PEP + ADP. However, when the pH of the PEP solutions was not corrected for the addition of PEP, strong channel inhibition was observed as a result of the well-known action of protons to inhibit KATP. In cell-attached recordings, perifusing either a PK activator or an inhibitor had little or no effect on KATP channel closure by glucose, further suggesting that PK is not an important regulator of KATP. In contrast, addition of mitochondrial inhibitors robustly increased KATP activity. Finally, by measuring the [ATP]/[ADP] responses to imposed calcium oscillations in mouse beta cells, we found that oxidative phosphorylation could raise [ATP]/[ADP] even when ADP was at its nadir during the burst silent phase, in agreement with our mathematical model. These results indicate that ATP produced by mitochondrial oxidative phosphorylation is the primary controller of KATP in pancreatic beta cells
publishDate 2023
dc.date.none.fl_str_mv 2023-11-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/239102
Corradi, Jeremias; Thompson, Benjamin; Fletcher, Patrick A.; Bertram, Richard; Sherman, Arthur S.; et al.; K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production; John Wiley & Sons Inc.; The Journal of Physiology; 601; 24; 17-11-2023; 5655-5667
1469-7793
0022-3751
CONICET Digital
CONICET
url http://hdl.handle.net/11336/239102
identifier_str_mv Corradi, Jeremias; Thompson, Benjamin; Fletcher, Patrick A.; Bertram, Richard; Sherman, Arthur S.; et al.; K ATP channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production; John Wiley & Sons Inc.; The Journal of Physiology; 601; 24; 17-11-2023; 5655-5667
1469-7793
0022-3751
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP284982
info:eu-repo/semantics/altIdentifier/doi/10.1113/JP284982
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc.
publisher.none.fl_str_mv John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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