Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection

Autores
Palmer, Clovis S.; Duette, Gabriel; Wagner, Marc C. E.; Henstridge, Darren C.; Saleh, Suah; Pereira, Candida; Zhou, Jingling; Simar, David; Lewin, Sharon R.; Ostrowski, Matias; McCune, Joseph M.; Crowe, Suzanne M.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High glucose transporter 1 (Glut1) surface expression is associated with increased glycolytic activity in activated CD4+ T cells. Phosphatidylinositide 3-kinases (PI3K) activation measured by p-Akt and OX40 is elevated in CD4+Glut1+ T cells from HIV+ subjects. TCR engagement of CD4+Glut1+ T cells from HIV+ subjects demonstrates hyperresponsive PI3K-mammalian target of rapamycin signaling. High basal Glut1 and OX40 on CD4+ T cells from combination antiretroviral therapy (cART)-treated HIV+ patients represent a sufficiently metabolically active state permissive for HIV infection in vitro without external stimuli. The majority of CD4+OX40+ T cells express Glut1, thus OX40 rather than Glut1 itself may facilitate HIV infection. Furthermore, infection of CD4+ T cells is limited by p110γ PI3K inhibition. Modulating glucose metabolism may limit cellular activation and prevent residual HIV replication in ‘virologically suppressed’ cART-treated HIV+ persons.
Fil: Palmer, Clovis S.. Burnet Institute; Australia. Monash University; Australia. University of Melbourne; Australia
Fil: Duette, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Wagner, Marc C. E.. No especifíca;
Fil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; Australia
Fil: Saleh, Suah. Burnet Institute; Australia. University of Melbourne; Australia
Fil: Pereira, Candida. Burnet Institute; Australia. University of Melbourne; Australia. Monash University; Australia
Fil: Zhou, Jingling. Burnet Institute; Australia
Fil: Simar, David. University of New South Wales; Australia
Fil: Lewin, Sharon R.. Monash University; Australia. University of Melbourne; Australia
Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: McCune, Joseph M.. University of California; Estados Unidos
Fil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia
Materia
CANCER
CD4 T CELLS
GLUT1
HIV
IMMUNOMETABOLISM
MTOR
PI3K
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66268
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66268
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infectionPalmer, Clovis S.Duette, GabrielWagner, Marc C. E.Henstridge, Darren C.Saleh, SuahPereira, CandidaZhou, JinglingSimar, DavidLewin, Sharon R.Ostrowski, MatiasMcCune, Joseph M.Crowe, Suzanne M.CANCERCD4 T CELLSGLUT1HIVIMMUNOMETABOLISMMTORPI3Khttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3High glucose transporter 1 (Glut1) surface expression is associated with increased glycolytic activity in activated CD4+ T cells. Phosphatidylinositide 3-kinases (PI3K) activation measured by p-Akt and OX40 is elevated in CD4+Glut1+ T cells from HIV+ subjects. TCR engagement of CD4+Glut1+ T cells from HIV+ subjects demonstrates hyperresponsive PI3K-mammalian target of rapamycin signaling. High basal Glut1 and OX40 on CD4+ T cells from combination antiretroviral therapy (cART)-treated HIV+ patients represent a sufficiently metabolically active state permissive for HIV infection in vitro without external stimuli. The majority of CD4+OX40+ T cells express Glut1, thus OX40 rather than Glut1 itself may facilitate HIV infection. Furthermore, infection of CD4+ T cells is limited by p110γ PI3K inhibition. Modulating glucose metabolism may limit cellular activation and prevent residual HIV replication in ‘virologically suppressed’ cART-treated HIV+ persons.Fil: Palmer, Clovis S.. Burnet Institute; Australia. Monash University; Australia. University of Melbourne; AustraliaFil: Duette, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Wagner, Marc C. E.. No especifíca;Fil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; AustraliaFil: Saleh, Suah. Burnet Institute; Australia. University of Melbourne; AustraliaFil: Pereira, Candida. Burnet Institute; Australia. University of Melbourne; Australia. Monash University; AustraliaFil: Zhou, Jingling. Burnet Institute; AustraliaFil: Simar, David. University of New South Wales; AustraliaFil: Lewin, Sharon R.. Monash University; Australia. University of Melbourne; AustraliaFil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: McCune, Joseph M.. University of California; Estados UnidosFil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; AustraliaElsevier Science2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66268Palmer, Clovis S.; Duette, Gabriel; Wagner, Marc C. E.; Henstridge, Darren C.; Saleh, Suah; et al.; Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection; Elsevier Science; FEBS Letters; 591; 20; 10-2017; 3319-33320014-5793CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/1873-3468.12843info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.12843info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:05Zoai:ri.conicet.gov.ar:11336/66268instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:05.682CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
title Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
spellingShingle Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
Palmer, Clovis S.
CANCER
CD4 T CELLS
GLUT1
HIV
IMMUNOMETABOLISM
MTOR
PI3K
title_short Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
title_full Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
title_fullStr Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
title_full_unstemmed Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
title_sort Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection
dc.creator.none.fl_str_mv Palmer, Clovis S.
Duette, Gabriel
Wagner, Marc C. E.
Henstridge, Darren C.
Saleh, Suah
Pereira, Candida
Zhou, Jingling
Simar, David
Lewin, Sharon R.
Ostrowski, Matias
McCune, Joseph M.
Crowe, Suzanne M.
author Palmer, Clovis S.
author_facet Palmer, Clovis S.
Duette, Gabriel
Wagner, Marc C. E.
Henstridge, Darren C.
Saleh, Suah
Pereira, Candida
Zhou, Jingling
Simar, David
Lewin, Sharon R.
Ostrowski, Matias
McCune, Joseph M.
Crowe, Suzanne M.
author_role author
author2 Duette, Gabriel
Wagner, Marc C. E.
Henstridge, Darren C.
Saleh, Suah
Pereira, Candida
Zhou, Jingling
Simar, David
Lewin, Sharon R.
Ostrowski, Matias
McCune, Joseph M.
Crowe, Suzanne M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANCER
CD4 T CELLS
GLUT1
HIV
IMMUNOMETABOLISM
MTOR
PI3K
topic CANCER
CD4 T CELLS
GLUT1
HIV
IMMUNOMETABOLISM
MTOR
PI3K
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High glucose transporter 1 (Glut1) surface expression is associated with increased glycolytic activity in activated CD4+ T cells. Phosphatidylinositide 3-kinases (PI3K) activation measured by p-Akt and OX40 is elevated in CD4+Glut1+ T cells from HIV+ subjects. TCR engagement of CD4+Glut1+ T cells from HIV+ subjects demonstrates hyperresponsive PI3K-mammalian target of rapamycin signaling. High basal Glut1 and OX40 on CD4+ T cells from combination antiretroviral therapy (cART)-treated HIV+ patients represent a sufficiently metabolically active state permissive for HIV infection in vitro without external stimuli. The majority of CD4+OX40+ T cells express Glut1, thus OX40 rather than Glut1 itself may facilitate HIV infection. Furthermore, infection of CD4+ T cells is limited by p110γ PI3K inhibition. Modulating glucose metabolism may limit cellular activation and prevent residual HIV replication in ‘virologically suppressed’ cART-treated HIV+ persons.
Fil: Palmer, Clovis S.. Burnet Institute; Australia. Monash University; Australia. University of Melbourne; Australia
Fil: Duette, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Wagner, Marc C. E.. No especifíca;
Fil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; Australia
Fil: Saleh, Suah. Burnet Institute; Australia. University of Melbourne; Australia
Fil: Pereira, Candida. Burnet Institute; Australia. University of Melbourne; Australia. Monash University; Australia
Fil: Zhou, Jingling. Burnet Institute; Australia
Fil: Simar, David. University of New South Wales; Australia
Fil: Lewin, Sharon R.. Monash University; Australia. University of Melbourne; Australia
Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: McCune, Joseph M.. University of California; Estados Unidos
Fil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia
description High glucose transporter 1 (Glut1) surface expression is associated with increased glycolytic activity in activated CD4+ T cells. Phosphatidylinositide 3-kinases (PI3K) activation measured by p-Akt and OX40 is elevated in CD4+Glut1+ T cells from HIV+ subjects. TCR engagement of CD4+Glut1+ T cells from HIV+ subjects demonstrates hyperresponsive PI3K-mammalian target of rapamycin signaling. High basal Glut1 and OX40 on CD4+ T cells from combination antiretroviral therapy (cART)-treated HIV+ patients represent a sufficiently metabolically active state permissive for HIV infection in vitro without external stimuli. The majority of CD4+OX40+ T cells express Glut1, thus OX40 rather than Glut1 itself may facilitate HIV infection. Furthermore, infection of CD4+ T cells is limited by p110γ PI3K inhibition. Modulating glucose metabolism may limit cellular activation and prevent residual HIV replication in ‘virologically suppressed’ cART-treated HIV+ persons.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66268
Palmer, Clovis S.; Duette, Gabriel; Wagner, Marc C. E.; Henstridge, Darren C.; Saleh, Suah; et al.; Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection; Elsevier Science; FEBS Letters; 591; 20; 10-2017; 3319-3332
0014-5793
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66268
identifier_str_mv Palmer, Clovis S.; Duette, Gabriel; Wagner, Marc C. E.; Henstridge, Darren C.; Saleh, Suah; et al.; Metabolically active CD4+ T cells expressing Glut1 and OX40 preferentially harbor HIV during in vitro infection; Elsevier Science; FEBS Letters; 591; 20; 10-2017; 3319-3332
0014-5793
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/1873-3468.12843
info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.12843
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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