Arabidopsis thaliana embryo sac mitochondrial membrane potential stain

Autores
Martin, María Victoria; Fiol, Diego Fernando; Zabaleta, Eduardo Julian; Pagnussat, Gabriela Carolina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibrium of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol, a distinctive feature of the early stages of programmed cell death. JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with intense red fluorescence. On the other hand, in mitochondria with low mitochondrial potential, JC-1 remains in the monomeric form, which exhibits only green fluorescence (Martin et al., 2013; Hauser et al., 2006). This protocol could be used in isolated mitochondria, and in a variety of cell types and different tissues of plants and other organism.
Fil: Martin, María Victoria. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fiol, Diego Fernando. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabaleta, Eduardo Julian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pagnussat, Gabriela Carolina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Mitochondrial membrane potential
Female gametophyte
Arabidopsis thaliana
Plant embryo sac
JC-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13204
Acceder
id CONICETDig_2487be21bfe451c29cf50af26c84dbb9
oai_identifier_str oai:ri.conicet.gov.ar:11336/13204
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arabidopsis thaliana embryo sac mitochondrial membrane potential stainMartin, María VictoriaFiol, Diego FernandoZabaleta, Eduardo JulianPagnussat, Gabriela CarolinaMitochondrial membrane potentialFemale gametophyteArabidopsis thalianaPlant embryo sacJC-1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibrium of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol, a distinctive feature of the early stages of programmed cell death. JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with intense red fluorescence. On the other hand, in mitochondria with low mitochondrial potential, JC-1 remains in the monomeric form, which exhibits only green fluorescence (Martin et al., 2013; Hauser et al., 2006). This protocol could be used in isolated mitochondria, and in a variety of cell types and different tissues of plants and other organism.Fil: Martin, María Victoria. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fiol, Diego Fernando. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zabaleta, Eduardo Julian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pagnussat, Gabriela Carolina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBio-protocol LLC2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13204Martin, María Victoria; Fiol, Diego Fernando; Zabaleta, Eduardo Julian; Pagnussat, Gabriela Carolina; Arabidopsis thaliana embryo sac mitochondrial membrane potential stain; Bio-protocol LLC; Bio-protocol; 4; 10; 5-2014; 1-52331-8325enginfo:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1128info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1128info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:31:21Zoai:ri.conicet.gov.ar:11336/13204instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:31:21.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
title Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
spellingShingle Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
Martin, María Victoria
Mitochondrial membrane potential
Female gametophyte
Arabidopsis thaliana
Plant embryo sac
JC-1
title_short Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
title_full Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
title_fullStr Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
title_full_unstemmed Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
title_sort Arabidopsis thaliana embryo sac mitochondrial membrane potential stain
dc.creator.none.fl_str_mv Martin, María Victoria
Fiol, Diego Fernando
Zabaleta, Eduardo Julian
Pagnussat, Gabriela Carolina
author Martin, María Victoria
author_facet Martin, María Victoria
Fiol, Diego Fernando
Zabaleta, Eduardo Julian
Pagnussat, Gabriela Carolina
author_role author
author2 Fiol, Diego Fernando
Zabaleta, Eduardo Julian
Pagnussat, Gabriela Carolina
author2_role author
author
author
dc.subject.none.fl_str_mv Mitochondrial membrane potential
Female gametophyte
Arabidopsis thaliana
Plant embryo sac
JC-1
topic Mitochondrial membrane potential
Female gametophyte
Arabidopsis thaliana
Plant embryo sac
JC-1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibrium of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol, a distinctive feature of the early stages of programmed cell death. JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with intense red fluorescence. On the other hand, in mitochondria with low mitochondrial potential, JC-1 remains in the monomeric form, which exhibits only green fluorescence (Martin et al., 2013; Hauser et al., 2006). This protocol could be used in isolated mitochondria, and in a variety of cell types and different tissues of plants and other organism.
Fil: Martin, María Victoria. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fiol, Diego Fernando. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zabaleta, Eduardo Julian. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pagnussat, Gabriela Carolina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibrium of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol, a distinctive feature of the early stages of programmed cell death. JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with intense red fluorescence. On the other hand, in mitochondria with low mitochondrial potential, JC-1 remains in the monomeric form, which exhibits only green fluorescence (Martin et al., 2013; Hauser et al., 2006). This protocol could be used in isolated mitochondria, and in a variety of cell types and different tissues of plants and other organism.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13204
Martin, María Victoria; Fiol, Diego Fernando; Zabaleta, Eduardo Julian; Pagnussat, Gabriela Carolina; Arabidopsis thaliana embryo sac mitochondrial membrane potential stain; Bio-protocol LLC; Bio-protocol; 4; 10; 5-2014; 1-5
2331-8325
url http://hdl.handle.net/11336/13204
identifier_str_mv Martin, María Victoria; Fiol, Diego Fernando; Zabaleta, Eduardo Julian; Pagnussat, Gabriela Carolina; Arabidopsis thaliana embryo sac mitochondrial membrane potential stain; Bio-protocol LLC; Bio-protocol; 4; 10; 5-2014; 1-5
2331-8325
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1128
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1128
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-protocol LLC
publisher.none.fl_str_mv Bio-protocol LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083449390104576
score 13.22299

Publicaciones similares