Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana

Autores
Panoli, Aneesh; Martin, María Victoria; Alandete Saez, Mónica; Simon, Marissa; Neff, Cristina; Swarup, Ranjan; Bellido, Andres; Yuan, Li; Pagnussat, Gabriela Carolina; Sundaresan, Venkatesan
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The female gametophyte of flowering plants, called the embryo sac, develops from a haploid cell named the functional megaspore, which is specified after meiosis by the diploid sporophyte. In Arabidopsis, the functional megaspore undergoes three syncitial mitotic divisions followed by cellularization to form seven cells of four cell types including two female gametes. The plant hormone auxin is important for sporophytic developmental processes, and auxin levels are known to be regulated by biosynthesis and transport. Here, we investigated the role of auxin biosynthetic genes and auxin influx carriers in embryo sac development. We find that genes from the YUCCA/TAA pathway (YUC1, YUC2, YUC8, TAA1, TAR2) are expressed asymmetrically in the developing ovule and embryo sac from the twonuclear syncitial stage until cellularization. Mutants for YUC1 and YUC2 exhibited defects in cell specification, whereas mutations in YUC8, as well as mutations in TAA1 and TAR2, caused defects in nuclear proliferation, vacuole formation and anisotropic growth of the embryo sac. Additionally, expression of the auxin influx carriers AUX1 and LAX1 were observed at the micropylar pole of the embryo sac and in the adjacent cells of the ovule, and the aux1 lax1 lax2 triple mutant shows multiple gametophyte defects. These results indicate that both localized auxin biosynthesis and auxin import, are required for mitotic divisions, cell expansion and patterning during embryo sac development.
Fil: Panoli, Aneesh. University Of California At Davis; Estados Unidos
Fil: Martin, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Alandete Saez, Mónica. University Of California At Davis; Estados Unidos
Fil: Simon, Marissa. University Of California At Davis; Estados Unidos
Fil: Neff, Cristina. University Of California At Davis; Estados Unidos
Fil: Swarup, Ranjan . The University Of Nottingham; Reino Unido
Fil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Yuan, Li. University Of California At Davis; Estados Unidos
Fil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sundaresan, Venkatesan. University Of California At Davis; Estados Unidos
Materia
EMBRYO SAC DEVELOPMENT
AUXINS
FEMALE GAMETOPHYTE
MITOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10708
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10708
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis ThalianaPanoli, AneeshMartin, María VictoriaAlandete Saez, MónicaSimon, MarissaNeff, CristinaSwarup, Ranjan Bellido, AndresYuan, LiPagnussat, Gabriela CarolinaSundaresan, VenkatesanEMBRYO SAC DEVELOPMENTAUXINSFEMALE GAMETOPHYTEMITOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The female gametophyte of flowering plants, called the embryo sac, develops from a haploid cell named the functional megaspore, which is specified after meiosis by the diploid sporophyte. In Arabidopsis, the functional megaspore undergoes three syncitial mitotic divisions followed by cellularization to form seven cells of four cell types including two female gametes. The plant hormone auxin is important for sporophytic developmental processes, and auxin levels are known to be regulated by biosynthesis and transport. Here, we investigated the role of auxin biosynthetic genes and auxin influx carriers in embryo sac development. We find that genes from the YUCCA/TAA pathway (YUC1, YUC2, YUC8, TAA1, TAR2) are expressed asymmetrically in the developing ovule and embryo sac from the twonuclear syncitial stage until cellularization. Mutants for YUC1 and YUC2 exhibited defects in cell specification, whereas mutations in YUC8, as well as mutations in TAA1 and TAR2, caused defects in nuclear proliferation, vacuole formation and anisotropic growth of the embryo sac. Additionally, expression of the auxin influx carriers AUX1 and LAX1 were observed at the micropylar pole of the embryo sac and in the adjacent cells of the ovule, and the aux1 lax1 lax2 triple mutant shows multiple gametophyte defects. These results indicate that both localized auxin biosynthesis and auxin import, are required for mitotic divisions, cell expansion and patterning during embryo sac development.Fil: Panoli, Aneesh. University Of California At Davis; Estados UnidosFil: Martin, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Alandete Saez, Mónica. University Of California At Davis; Estados UnidosFil: Simon, Marissa. University Of California At Davis; Estados UnidosFil: Neff, Cristina. University Of California At Davis; Estados UnidosFil: Swarup, Ranjan . The University Of Nottingham; Reino UnidoFil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Yuan, Li. University Of California At Davis; Estados UnidosFil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Sundaresan, Venkatesan. University Of California At Davis; Estados UnidosPublic Library Of Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10708Panoli, Aneesh; Martin, María Victoria; Alandete Saez, Mónica; Simon, Marissa; Neff, Cristina; et al.; Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana; Public Library Of Science; Plos One; 10; 5; 5-2015; 1-23; e01261641932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0126164info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126164info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25970627/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:55Zoai:ri.conicet.gov.ar:11336/10708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:55.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
title Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
spellingShingle Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
Panoli, Aneesh
EMBRYO SAC DEVELOPMENT
AUXINS
FEMALE GAMETOPHYTE
MITOSIS
title_short Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
title_full Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
title_fullStr Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
title_full_unstemmed Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
title_sort Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana
dc.creator.none.fl_str_mv Panoli, Aneesh
Martin, María Victoria
Alandete Saez, Mónica
Simon, Marissa
Neff, Cristina
Swarup, Ranjan
Bellido, Andres
Yuan, Li
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
author Panoli, Aneesh
author_facet Panoli, Aneesh
Martin, María Victoria
Alandete Saez, Mónica
Simon, Marissa
Neff, Cristina
Swarup, Ranjan
Bellido, Andres
Yuan, Li
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
author_role author
author2 Martin, María Victoria
Alandete Saez, Mónica
Simon, Marissa
Neff, Cristina
Swarup, Ranjan
Bellido, Andres
Yuan, Li
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EMBRYO SAC DEVELOPMENT
AUXINS
FEMALE GAMETOPHYTE
MITOSIS
topic EMBRYO SAC DEVELOPMENT
AUXINS
FEMALE GAMETOPHYTE
MITOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The female gametophyte of flowering plants, called the embryo sac, develops from a haploid cell named the functional megaspore, which is specified after meiosis by the diploid sporophyte. In Arabidopsis, the functional megaspore undergoes three syncitial mitotic divisions followed by cellularization to form seven cells of four cell types including two female gametes. The plant hormone auxin is important for sporophytic developmental processes, and auxin levels are known to be regulated by biosynthesis and transport. Here, we investigated the role of auxin biosynthetic genes and auxin influx carriers in embryo sac development. We find that genes from the YUCCA/TAA pathway (YUC1, YUC2, YUC8, TAA1, TAR2) are expressed asymmetrically in the developing ovule and embryo sac from the twonuclear syncitial stage until cellularization. Mutants for YUC1 and YUC2 exhibited defects in cell specification, whereas mutations in YUC8, as well as mutations in TAA1 and TAR2, caused defects in nuclear proliferation, vacuole formation and anisotropic growth of the embryo sac. Additionally, expression of the auxin influx carriers AUX1 and LAX1 were observed at the micropylar pole of the embryo sac and in the adjacent cells of the ovule, and the aux1 lax1 lax2 triple mutant shows multiple gametophyte defects. These results indicate that both localized auxin biosynthesis and auxin import, are required for mitotic divisions, cell expansion and patterning during embryo sac development.
Fil: Panoli, Aneesh. University Of California At Davis; Estados Unidos
Fil: Martin, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Alandete Saez, Mónica. University Of California At Davis; Estados Unidos
Fil: Simon, Marissa. University Of California At Davis; Estados Unidos
Fil: Neff, Cristina. University Of California At Davis; Estados Unidos
Fil: Swarup, Ranjan . The University Of Nottingham; Reino Unido
Fil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Yuan, Li. University Of California At Davis; Estados Unidos
Fil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Sundaresan, Venkatesan. University Of California At Davis; Estados Unidos
description The female gametophyte of flowering plants, called the embryo sac, develops from a haploid cell named the functional megaspore, which is specified after meiosis by the diploid sporophyte. In Arabidopsis, the functional megaspore undergoes three syncitial mitotic divisions followed by cellularization to form seven cells of four cell types including two female gametes. The plant hormone auxin is important for sporophytic developmental processes, and auxin levels are known to be regulated by biosynthesis and transport. Here, we investigated the role of auxin biosynthetic genes and auxin influx carriers in embryo sac development. We find that genes from the YUCCA/TAA pathway (YUC1, YUC2, YUC8, TAA1, TAR2) are expressed asymmetrically in the developing ovule and embryo sac from the twonuclear syncitial stage until cellularization. Mutants for YUC1 and YUC2 exhibited defects in cell specification, whereas mutations in YUC8, as well as mutations in TAA1 and TAR2, caused defects in nuclear proliferation, vacuole formation and anisotropic growth of the embryo sac. Additionally, expression of the auxin influx carriers AUX1 and LAX1 were observed at the micropylar pole of the embryo sac and in the adjacent cells of the ovule, and the aux1 lax1 lax2 triple mutant shows multiple gametophyte defects. These results indicate that both localized auxin biosynthesis and auxin import, are required for mitotic divisions, cell expansion and patterning during embryo sac development.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10708
Panoli, Aneesh; Martin, María Victoria; Alandete Saez, Mónica; Simon, Marissa; Neff, Cristina; et al.; Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana; Public Library Of Science; Plos One; 10; 5; 5-2015; 1-23; e0126164
1932-6203
url http://hdl.handle.net/11336/10708
identifier_str_mv Panoli, Aneesh; Martin, María Victoria; Alandete Saez, Mónica; Simon, Marissa; Neff, Cristina; et al.; Auxin import and local Auxin Biosynthesis are required for Mitotic Divisions, Cell Expansion and Cell Specification during female Gametophyte development in Arabidopsis Thaliana; Public Library Of Science; Plos One; 10; 5; 5-2015; 1-23; e0126164
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0126164
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126164
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25970627/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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