A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana

Autores
Boavida, Leonor C.; Shuai, Bin; Yu, Hee Ju; Pagnussat, Gabriela Carolina; Sundaresan, Venkatesan; McCormick, Sheila
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since ∼10% of the genes are expressed in the male gametophyte and ∼9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are male gametophytic-specific mutations, while the others also affect female transmission. Genomic sequences flanking both sides of the Ds element were recovered for 39 lines; for 16 the Ds elements were inserted in or close to coding regions, while 7 were located in intergenic/unannotated regions of the genome. For the remaining 16 lines, chromosomal rearrangements such as translocations or deletions, ranging between 30 and 500 kb, were associated with the transposition event. The mutants were classified into five groups according to the developmental processes affected; these ranged from defects in early stages of gametogenesis to later defects affecting pollen germination, pollen tube growth, polarity or guidance, or pollen tube - embryo sac interactions or fertilization. The isolated mutants carry Ds insertions in genes with diverse biological functions and potentially specify new functions for several unannotated or unknown proteins.
Fil: Boavida, Leonor C.. United States Department of Agriculture; Estados Unidos
Fil: Shuai, Bin. United States Department of Agriculture; Estados Unidos
Fil: Yu, Hee Ju. University of California at Davis; Estados Unidos
Fil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Sundaresan, Venkatesan. University of California at Davis; Estados Unidos
Fil: McCormick, Sheila. United States Department of Agriculture; Estados Unidos
Materia
Gametophyte
Development
Mutants
Arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/134788
Acceder
id CONICETDig_ee4286df5a0272c06145fbf77e565b4a
oai_identifier_str oai:ri.conicet.gov.ar:11336/134788
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thalianaBoavida, Leonor C.Shuai, BinYu, Hee JuPagnussat, Gabriela CarolinaSundaresan, VenkatesanMcCormick, SheilaGametophyteDevelopmentMutantsArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since ∼10% of the genes are expressed in the male gametophyte and ∼9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are male gametophytic-specific mutations, while the others also affect female transmission. Genomic sequences flanking both sides of the Ds element were recovered for 39 lines; for 16 the Ds elements were inserted in or close to coding regions, while 7 were located in intergenic/unannotated regions of the genome. For the remaining 16 lines, chromosomal rearrangements such as translocations or deletions, ranging between 30 and 500 kb, were associated with the transposition event. The mutants were classified into five groups according to the developmental processes affected; these ranged from defects in early stages of gametogenesis to later defects affecting pollen germination, pollen tube growth, polarity or guidance, or pollen tube - embryo sac interactions or fertilization. The isolated mutants carry Ds insertions in genes with diverse biological functions and potentially specify new functions for several unannotated or unknown proteins.Fil: Boavida, Leonor C.. United States Department of Agriculture; Estados UnidosFil: Shuai, Bin. United States Department of Agriculture; Estados UnidosFil: Yu, Hee Ju. University of California at Davis; Estados UnidosFil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Sundaresan, Venkatesan. University of California at Davis; Estados UnidosFil: McCormick, Sheila. United States Department of Agriculture; Estados UnidosGenetics Society of America2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/134788Boavida, Leonor C.; Shuai, Bin; Yu, Hee Ju; Pagnussat, Gabriela Carolina; Sundaresan, Venkatesan; et al.; A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana; Genetics Society of America; Genetics; 181; 4; 1-4-2009; 1369-13850016-6731CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1534/genetics.108.090852info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/genetics/article/181/4/1369/6081291info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:13Zoai:ri.conicet.gov.ar:11336/134788instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:13.621CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
title A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
spellingShingle A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
Boavida, Leonor C.
Gametophyte
Development
Mutants
Arabidopsis
title_short A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
title_full A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
title_fullStr A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
title_full_unstemmed A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
title_sort A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana
dc.creator.none.fl_str_mv Boavida, Leonor C.
Shuai, Bin
Yu, Hee Ju
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
McCormick, Sheila
author Boavida, Leonor C.
author_facet Boavida, Leonor C.
Shuai, Bin
Yu, Hee Ju
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
McCormick, Sheila
author_role author
author2 Shuai, Bin
Yu, Hee Ju
Pagnussat, Gabriela Carolina
Sundaresan, Venkatesan
McCormick, Sheila
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Gametophyte
Development
Mutants
Arabidopsis
topic Gametophyte
Development
Mutants
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since ∼10% of the genes are expressed in the male gametophyte and ∼9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are male gametophytic-specific mutations, while the others also affect female transmission. Genomic sequences flanking both sides of the Ds element were recovered for 39 lines; for 16 the Ds elements were inserted in or close to coding regions, while 7 were located in intergenic/unannotated regions of the genome. For the remaining 16 lines, chromosomal rearrangements such as translocations or deletions, ranging between 30 and 500 kb, were associated with the transposition event. The mutants were classified into five groups according to the developmental processes affected; these ranged from defects in early stages of gametogenesis to later defects affecting pollen germination, pollen tube growth, polarity or guidance, or pollen tube - embryo sac interactions or fertilization. The isolated mutants carry Ds insertions in genes with diverse biological functions and potentially specify new functions for several unannotated or unknown proteins.
Fil: Boavida, Leonor C.. United States Department of Agriculture; Estados Unidos
Fil: Shuai, Bin. United States Department of Agriculture; Estados Unidos
Fil: Yu, Hee Ju. University of California at Davis; Estados Unidos
Fil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Sundaresan, Venkatesan. University of California at Davis; Estados Unidos
Fil: McCormick, Sheila. United States Department of Agriculture; Estados Unidos
description Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since ∼10% of the genes are expressed in the male gametophyte and ∼9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduced transmission through the male gametophyte. About half of these mutations are male gametophytic-specific mutations, while the others also affect female transmission. Genomic sequences flanking both sides of the Ds element were recovered for 39 lines; for 16 the Ds elements were inserted in or close to coding regions, while 7 were located in intergenic/unannotated regions of the genome. For the remaining 16 lines, chromosomal rearrangements such as translocations or deletions, ranging between 30 and 500 kb, were associated with the transposition event. The mutants were classified into five groups according to the developmental processes affected; these ranged from defects in early stages of gametogenesis to later defects affecting pollen germination, pollen tube growth, polarity or guidance, or pollen tube - embryo sac interactions or fertilization. The isolated mutants carry Ds insertions in genes with diverse biological functions and potentially specify new functions for several unannotated or unknown proteins.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/134788
Boavida, Leonor C.; Shuai, Bin; Yu, Hee Ju; Pagnussat, Gabriela Carolina; Sundaresan, Venkatesan; et al.; A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana; Genetics Society of America; Genetics; 181; 4; 1-4-2009; 1369-1385
0016-6731
CONICET Digital
CONICET
url http://hdl.handle.net/11336/134788
identifier_str_mv Boavida, Leonor C.; Shuai, Bin; Yu, Hee Ju; Pagnussat, Gabriela Carolina; Sundaresan, Venkatesan; et al.; A collection of Ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana; Genetics Society of America; Genetics; 181; 4; 1-4-2009; 1369-1385
0016-6731
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1534/genetics.108.090852
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/genetics/article/181/4/1369/6081291
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Genetics Society of America
publisher.none.fl_str_mv Genetics Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269568473497600
score 13.13397

Publicaciones similares