A simply method for the addition of rotenone in Arabidopsis thaliana leaves

Autores
Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
Fil: Maliandi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Rotenone
Arabidopsis Thaliana
Mitochondria
Mitochondrial Respiration
Complex I
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7826
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7826
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A simply method for the addition of rotenone in Arabidopsis thaliana leavesMaliandi, María VictoriaRius, Sebastian PabloBusi, María VictoriaGomez Casati, Diego FabianRotenoneArabidopsis ThalianaMitochondriaMitochondrial RespirationComplex Ihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.Fil: Maliandi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); ArgentinaFil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaTaylor & Francis2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7826Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian; A simply method for the addition of rotenone in Arabidopsis thaliana leaves; Taylor & Francis; Plant Signaling and Behavior; 10; 10; 7-2015; 1-31559-2316enginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883935/info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:41:17Zoai:ri.conicet.gov.ar:11336/7826instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:41:17.841CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A simply method for the addition of rotenone in Arabidopsis thaliana leaves
title A simply method for the addition of rotenone in Arabidopsis thaliana leaves
spellingShingle A simply method for the addition of rotenone in Arabidopsis thaliana leaves
Maliandi, María Victoria
Rotenone
Arabidopsis Thaliana
Mitochondria
Mitochondrial Respiration
Complex I
title_short A simply method for the addition of rotenone in Arabidopsis thaliana leaves
title_full A simply method for the addition of rotenone in Arabidopsis thaliana leaves
title_fullStr A simply method for the addition of rotenone in Arabidopsis thaliana leaves
title_full_unstemmed A simply method for the addition of rotenone in Arabidopsis thaliana leaves
title_sort A simply method for the addition of rotenone in Arabidopsis thaliana leaves
dc.creator.none.fl_str_mv Maliandi, María Victoria
Rius, Sebastian Pablo
Busi, María Victoria
Gomez Casati, Diego Fabian
author Maliandi, María Victoria
author_facet Maliandi, María Victoria
Rius, Sebastian Pablo
Busi, María Victoria
Gomez Casati, Diego Fabian
author_role author
author2 Rius, Sebastian Pablo
Busi, María Victoria
Gomez Casati, Diego Fabian
author2_role author
author
author
dc.subject.none.fl_str_mv Rotenone
Arabidopsis Thaliana
Mitochondria
Mitochondrial Respiration
Complex I
topic Rotenone
Arabidopsis Thaliana
Mitochondria
Mitochondrial Respiration
Complex I
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
Fil: Maliandi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús. Instituto de Investigaciones Biotecnológicas (sede Chascomús); Argentina
Fil: Rius, Sebastian Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7826
Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian; A simply method for the addition of rotenone in Arabidopsis thaliana leaves; Taylor & Francis; Plant Signaling and Behavior; 10; 10; 7-2015; 1-3
1559-2316
url http://hdl.handle.net/11336/7826
identifier_str_mv Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian; A simply method for the addition of rotenone in Arabidopsis thaliana leaves; Taylor & Francis; Plant Signaling and Behavior; 10; 10; 7-2015; 1-3
1559-2316
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883935/
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597565927325696
score 12.976206

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